Characterization of Hungarian isolates of zucchini yellow mosaic virus (ZYMV, potyvirus) transmitted by seeds of <i>Cucurbita pepo</i> var <i>Styriaca</i>

Tóbiás, István; Palkovics, László

doi:10.1002/ps.645

Cited by 27 publications

(16 citation statements)

References 20 publications

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“…High similarity was also observed between the Saudi ZYMV isolates and isolates from distant countries (Germany, Hungary, Austria, and Slovenia). The occurrence of ZYMV isolates that have nucleotide sequences similar to the Saudi isolates in these countries may suggest the common origin of distantly distributed isolates, which can be attributed to the widespread seed transmission of this viral isolate as has been reported in previous studies (Tóbiás and Palkovics, 2003;Safaeizadeh, 2008).…”

Section: Isolation Detection and Host Reaction Of The Causal Virussupporting

confidence: 58%

Characterization of different isolates of Zucchini yellow mosaic virus from cucurbits in Saudi Arabia

Al-Saleh¹,

Amer²,

Al-Shahwan³

et al. 2014

Afr. J. Microbiol. Res.

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Section: Isolation Detection and Host Reaction Of The Causal Virussupporting

confidence: 58%

Characterization of different isolates of Zucchini yellow mosaic virus from cucurbits in Saudi Arabia

Al-Saleh¹,

Amer²,

Al-Shahwan³

et al. 2014

Afr. J. Microbiol. Res.

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“…Furthermore, ZYMV isolates from Serbia were characterized by two specifically conserved amino acid residues in the N-terminal part of the CP, as was previously reported for other isolates from Central Europe (Glasa et al 2007;Tóbiás and Palkovics 2003). Sequence diversity among ZYMV isolates has been reported in Australia (Coutts et al 2011b), Vietnam (Ha et al 2008) and Iran (Bananej et al 2008) with the greatest level of genetic diversity, including the deepest phylogenetic split, which has been seen in Asia (particularly China) (Simmons et al 2008).…”

Section: Discussionmentioning

confidence: 71%

“…Sequence diversity among ZYMV isolates has been reported in Australia (Coutts et al 2011b), Vietnam (Ha et al 2008) and Iran (Bananej et al 2008) with the greatest level of genetic diversity, including the deepest phylogenetic split, which has been seen in Asia (particularly China) (Simmons et al 2008). The lack of diversity has been reported among Central European isolates (Glasa et al 2007;Tóbiás and Palkovics 2003) and Martinique (Desbiez et al 1996) as well. The analysis performed in this study showed low diversity among Serbian ZYMV isolates originating from different localities and/or hosts and highlighted the close relatedness of Serbian and other Central European isolates of ZYMV.…”

Section: Discussionmentioning

confidence: 91%

“…This situation might be explained by the fact that so far, pumpkin and squash have been cultivated from seed supply produced in Serbia. Considering long-distance spread of ZYMV that may be the result of vertical transmission through infected seeds (Desbiez et al 2002;Simmons et al 2011;Tóbiás and Palkovics 2003), particular attention must be paid to the phytosanitary requirements for cucurbit seed imported to or produced in Serbia.…”

Section: Discussionmentioning

confidence: 99%

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Non-persistently aphid-borne viruses infecting pumpkin and squash in Serbia and partial characterization of Zucchini yellow mosaic virus isolates

et al. 2012

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Cucurbit species grown in the Vojvodina Province, Serbia, were surveyed for the incidence of Zucchini yellow mosaic virus (ZYMV), Watermelon mosaic virus (WMV), Cucumber mosaic virus (CMV), Squash mosaic virus (SqMV), Papaya ringspot virus (PRSV) and Tobacco ringspot virus (TRSV) from 2007 to 2009. Samples from more than 700 pumpkin, squash and bottle gourd plants with virus-like symptoms were analyzed by double-antibody sandwich (DAS)-ELISA. ZYMV, WMV and CMV were detected in 79.2, 32.2, and 12.8% of tested samples, respectively. WMV was prevalent in 2007 and ZYMV in 2008-09. Mixed infections were the most frequent type in 2007-08 in contrast to 2009 when single infection of ZYMV prevailed.ZYMV was the most widespread being found in 33 out of 39 inspected fields. Virus species identification was confirmed in selected samples by conventional reverse transcription-polymerase chain reaction (RT-PCR) and sequencing of their coat protein genes. By comparing the obtained virus isolate sequences with those available in GenBank, the identification of serologically detected viruses was confirmed. Phylogenetic analysis based on complete coat protein (CP) sequences highlighted that Serbian ZYMV isolates were closely related to other Central European ZYMV isolates. Finally, additional testing of ELISA-negative samples by RT-PCR using primers specific to six other mosaic viruses revealed the presence of Tomato spotted wilt virus (TSWV) in winter (Cucurbita maxima) and summer (C. pepo 'Beogradska') squash. This is the first report of TSWV natural occurrence on cucurbits in Serbia and on winter squash worldwide.

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“…U toku poslednje četiri decenije najveće štete usjevu tikava na Floridi nanose CMV, WMV, PRSV i ZYMV (Kucharek and Purcifull, 1997). U Mađarskoj su do 1995. godine, kad je prvi put otkriven ZYMV, najvažniji virusi tikava bili CMV i WMV (Tóbiás et al, 1996). Međutim, za kratko vrijeme, ZYMV se raširio u svim djelovima zemlje izazivajući zastrašujuće epidemije u usjevu različitih vrsta tikava, pa i uljane tikve (Tóbiás and Tulipán, 2002).…”

Section: Virusi Tikava I Njihov Značajunclassified

Diversity, biological and molecular characterization of cucurbit viruses and disease epidemiology in Serbia

Vučurović¹

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In the period from 2007 to 2011 constant high levels of viral infections were detected in 61 cucurbit growing localities in Serbia. The frequency of viral infection fluctuated over the years, ranging from 30-50% in 2007, to the extremely high 80% in the epidemic year of 2008, while it was constant and around 40% in the next two years. In the last year of investigation, the frequency reached a very high level of 60%. Serological testing of 1100 collected samples proved the presence of three viruses: Zucchini yellow mosaic virus (ZYMV), Watermelon mosaic virus (WMV) and Cucumber mosaic virus (CMV). Additional testing of symptomatic samples revealed the presence of Cucurbit aphid-borne yellows virus (CABYV), which represents the first detection of CABYV in Serbia, and Tomato spotted wilt virus (TSWV), which is the first report of TSWV on cucurbits in Serbia and the first report of Cucurbita maxima as natural host plant worldwide. ZYMV was the most prevalent virus during this five year survey. Mixed infections were the most frequent infection type in the first two years, while single infection prevailed in the last three years of investigation. During this investigation, transmission of ZYMV by seeds of C. pepo var. styriaca, C. maxima and C. moschata was proved. Both annual and perennial weeds, which are present during the establishment of cucurbit crops, and in the beginning stages of vegetation, were infected with ZYMV, WMV, CMV and TSWV. The most prevalent was TSWV which was detected in eight different weed species, the second most prevalent virus in weeds was CMV, detected in seven different weed species, while the prevalence of ZYMV and WMV was similar and they were detected in five and four weed species, respectively. A high diversity of aphids which are visiting cucurbit crops was detected through the determination of 57 aphid taxons. The most common aphid species were Aphis fabae, Anoecia corni, Aphis gossypii, Myzus persicae and Acyrthosiphon pisum. The greatest diversity and the largest number of aphids were detected in the first few weeks after plant sprouting. The detection and identification of the species level for all five cucurbit viruses in Serbia were confirmed by RT-PCR method and suitable specific primers. Bioassay on 45 test plants was used to determine phenotypic variability and host range for ZYMV, WMV and CMV. During the course of this investigation protocols for molecular detection of all five cucurbit viruses in Serbia were established or improved. Molecular characterizations of ZYMV, WMV, CMV, TSWV and CABYV were conducted by sequence and phylogenetic analyses. Sequence analysis of the CP gene revealed that Serbian ZYMV isolates belong to the Central European group of isolates. Sequence analysis based on NIb and CP genes showed that Serbian WMV isolates are grouped in two groups, and one of them represents a group of "emerging" strains containing isolates which are known for causing severe damaging symptoms on cucurbits, and this represents the first report of these isolates in Serbia. Bas...

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Characterization of Hungarian isolates of zucchini yellow mosaic virus (ZYMV, potyvirus) transmitted by seeds of Cucurbita pepo var Styriaca

Cited by 27 publications

References 20 publications

Characterization of different isolates of Zucchini yellow mosaic virus from cucurbits in Saudi Arabia

Characterization of different isolates of Zucchini yellow mosaic virus from cucurbits in Saudi Arabia

Non-persistently aphid-borne viruses infecting pumpkin and squash in Serbia and partial characterization of Zucchini yellow mosaic virus isolates

Diversity, biological and molecular characterization of cucurbit viruses and disease epidemiology in Serbia

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