Characterization of HPLC packings

Neue, Uwe D.; Tran, Kim Van; Iraneta, Pamela C.; Alden, Bonnie A.

doi:10.1002/jssc.200390025

Cited by 134 publications

(72 citation statements)

References 28 publications

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“…Studying the fullerenes' retention behavior elucidated the molecular shape recognition process on the stationary phase. Neue et al introduced naphthalene and acenaphthalene for measuring the hydrophobicity of the packing materials, basic compounds (propranolol and amitriptyline) for the determination of surface silanols, and butylparaben and dipropylphthalate as the markers of the polar selectivity of the stationary phase (38). Rohrschneider proposed a mixture of naphthalene, acetoanilide, phenol, and benzonitrile to characterize the retention behavior of C 18 columns in a reversed-phase system (39).…”

Section: Chromatographic Methodsmentioning

confidence: 99%

Peer Reviewed: Characterizing HPLC Stationary Phases

Krupczyńska¹,

Buszewski²

2004

Anal. Chem.

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great variety of stationary phases is available to analyze an even larger array of compounds. Stationary phases in which organic moieties (alkyl chains, aryl, amine, cholesterolic, phospholipids, phenol groups, etc.) are grafted onto a silica surface are the most popular. Sometimes the stationary phases themselves must be characterized, such as for quality control purposes. Often, interpreting chromatographic data is difficult because even columns that are "identical"-produced by the same manufacturer-exhibit different chromatographic properties. Determining the structure and properties of new stationary phases is also extremely important so that the retention mechanism, which plays a significant role in selectivity, can be described accurately. Chromatographic separation occurs because of the differences in the affinities of solute molecules for the stationary phase-retention is a process of solute transfer from a mobile phase onto/into a stationary phase (1).Column quality depends on a number of factors, the most important of which are homogeneity of both particle packing in the column bed and arrangement of the chemically bonded phase on the adsorbent surface. These factors determine the reproducibility of chromatographic data, and the large number of these factors is why there are a lot of methods for characterizing columns. Obviously, no ideal single technique can characterize all the © 2 0 0 4 A M E R

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Section: Chromatographic Methodsmentioning

confidence: 99%

Peer Reviewed: Characterizing HPLC Stationary Phases

Krupczyńska¹,

Buszewski²

2004

Anal. Chem.

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“…This degradation is accentuated at higher temperatures, low pH and highly aqueous mobile phases [27]. A test mixture as introduced by Neue [28] was used to evaluate the performance of the columns at room temperature.…”

Section: Column Test Studymentioning

confidence: 99%

Temperature and pH‐stability of commercial stationary phases

Teutenberg¹,

Hollebekkers²,

Wiese

et al. 2009

J of Separation Science

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Original PaperTemperature and pH-stability of commercial stationary phasesIn this paper, the temperature and pH stability of silica-based RP stationary phases were investigated. Furthermore, nonsiliceous phases like a polymeric column based on polystyrene divinylbenzene and a polybutadiene coated zirconium dioxide column were also included. The columns were heated up to 1508C at dynamic conditions, which means that the eluent consisting of water and methanol (90:10, v/v) was continuously purged through the packed bed. After every 5 h, the columns were cooled down to room temperature and the efficiency was measured by injecting a test sample based on the Neue test. It could be shown that some stationary phases exhibited a very good temperature stability at the test conditions specified above.

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“…Characterization of column quality is not a simple task, as a number of factors should be taken into account, affecting the intermolecular interactions between the analytes, the stationary phase and the mobile phases controlling the retention [2,3]. The type of the support material, the chemistry, arrangement and density of the stationary phase bonded on the adsorbent surface, the homogeneity of the chromatographic bed, particle size and porosity and other factors control the selectivity, efficiency and resolution of separation and reproducibility of chromatographic data.…”

Section: Introductionmentioning

confidence: 99%

“…In addition to simple measurements of the retention factors of test compounds in selected mobile phases, tests performed over a more or less broad range of mobile phase composition provide more complete information on the properties of stationary phases. Non-polar alkylbenzenes, various polar, weakly acidic or basic compounds, naphthalene sulphonic acids, oligomers and macromolecular test compounds are available for mapping various aspects of the retention properties of the chromatographic system [2][3][4].…”

Section: Introductionmentioning

confidence: 99%

Characterization of HPLC columns for two‐dimensional LC × LC separations of phenolic acids and flavonoids

Jandera

Vyňuchalová

Hájek

et al. 2008

Journal of Chemometrics

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The aim of the work is to contribute to rational selection of columns and mobile phases for 'orthogonal' twodimensional LC T LC operation of specific sample types, providing maximum increase in the peak capacity with respect to single-dimension HPLC. As a criterion of suitability, the dissimilarity of the separation selectivity of the phase separation systems was selected. For this purpose, correlations between the retention and molecular structure descriptors were used to characterize and compare the columns with respect to various types of selective interactions using the interaction indices as the general polarity scale characteristics and the linear free energy relationships (LFER) model employing selective molecular structural descriptors. Chemometric methods-cluster analysis using similarity dendrograms and multiple regression analysis-were used to classify several types of bonded C18, amide and polyethyleneglycol columns on the basis of differences in separation selectivities for selected phenolic and flavone standards. Systematic development of a comprehensive LC T LC method for two-dimensional separation of phenolic acids and flavone natural antioxidants was based on the selection of the combination of first-and second-dimension columns with low correlation of retention of representative standards providing a highly orthogonal two-dimensional separation system. The mobile phases providing best separation selectivity for phenolic compounds on each column tested were optimized using window-diagram optimization strategy. The final optimized conditions were employed for comprehensive LC T LC separations of natural antioxidants using a polyethylene glycol microcolumn in the first dimension and a short monolithic C18 column in the second dimension.

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Characterization of HPLC packings

Cited by 134 publications

References 28 publications

Peer Reviewed: Characterizing HPLC Stationary Phases

Peer Reviewed: Characterizing HPLC Stationary Phases

Temperature and pH‐stability of commercial stationary phases

Characterization of HPLC columns for two‐dimensional LC × LC separations of phenolic acids and flavonoids

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