Characterization of Grape Procyanidins Using High-Performance Liquid Chromatography/Mass Spectrometry and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

Yang, Ying; Chien, Mingjien

doi:10.1021/jf000316q

Cited by 104 publications

(91 citation statements)

References 39 publications

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“…Individual oligomers of vegetable tannins are well resolved in MALDI-TOF MS spectra with their molecular weights determinated in the analysis of condensed tannins (Ohnishi-Kameyama et al, 1997;Yang and Chien, 2000;Ishida et al, 2005). When MALDI-TOF MS is used to characterize tannins, the (Krueger et al, 2003).…”

Section: Maldi-tof Ms Analysismentioning

confidence: 99%

Tannins from Canarium album with potent antioxidant activity

Zhang¹,

Lin²

2008

J. Zhejiang Univ. Sci. B

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Abstract:The contents of total phenolics and extractable condensed tannins in the leaves, twigs and stem bark of Canarium album were determined. The structural heterogeneity of condensed tannins from stem bark was characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and nuclear magnetic resonance (NMR) analyses. The results show the predominance of signals representative of procyanidins and prodelphinidins. In addition, epicatechin and epigallocatechin polymers with galloylated procyanidin or prodelphinidin were also observed. The tannins were screened for their potential antioxidant activities using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing/antioxidant power (FRAP) model systems. Tannins extracted from leaves, twigs and stem bark all showed a very good DPPH radical scavenging activity and ferric reducing power.

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Section: Maldi-tof Ms Analysismentioning

confidence: 99%

Tannins from Canarium album with potent antioxidant activity

Zhang¹,

Lin²

2008

J. Zhejiang Univ. Sci. B

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“…The MALDI-TOF technique has advantages over other mass spectrometric systems, such as FAB, in sensitivity and mass range, and produces only a single ionization event (Krueger et al, 2000). Furthermore, it is highly tolerant toward contaminants, and is thus particularly suitable for the direct analysis of complex mixtures (Yang & Chien, 2000). Sugui et al (1999) studied by MALDI-TOF the anthocyanic profile of methanol extracts from five different A system with a nitrogen laser that operated at 337 nm, and an accelerating voltage 28 kV, was used, and a-cyano-4-hydroxycinnamic acid (CCA) was employed as the matrix.…”

Section: Application Of Maldi In the Study Of Polyphenolsmentioning

confidence: 99%

Mass spectrometry in grape and wine chemistry. Part I: Polyphenols

Flamini

2003

Mass Spectrometry Reviews

198

111

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Mass spectrometry, had and still has, a very important role for research and quality control in the viticulture and enology field, and its analytical power is relevant for structural studies on aroma and polyphenolic compounds. Polyphenols are responsible for the taste and color of wine, and confer astringency and structure to the beverage. The knowledge of the anthocyanic structure is very important to predict the aging attitude of wine, and to attempt to resolve problems about color stability. Moreover, polyphenols are the main compounds related to the benefits of wine consumption in the diet, because of their properties in the treatment of circulatory disorders such as capillary fragility, peripheral chronic venous insufficiency, and microangiopathy of the retina. Liquid Chromatography-Mass Spectrometry (LC-MS) techniques are nowadays the best analytical approach to study polyphenols in grape extracts and wine, and are the most effective tool in the study of the structure of anthocyanins. The MS/MS approach is a very powerful tool that permits anthocyanin aglycone and sugar moiety characterization. LC-MS allows the characterization of complex structures of grape polyphenols, such as procyanidins, proanthocyanidins, prodelphinidins, and tannins, and provides experimental evidence for structures that were previously only hypothesized. The matrix-assisted-laser-desorption-ionization-time-of-flight (MALDI-TOF) technique is suitable to determine the presence of molecules of higher molecular weight with high accuracy, and it has been applied with success to study procyanidin oligomers up to heptamers in the reflectron mode, and up to nonamers in the linear mode. The levels of resveratrol in wine, an important polyphenol well-known for its beneficial effects, have been determined by SPME and LC-MS, and the former approach led to the best results in terms of sensitivity.

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“…The HPLC -API-MS method has been applied qualitatively to a variety of other foods and beverages including peanut, tea, almonds, wine, grape juice, apple, cinnamon , blueberry and cranberry . Matrix assisted laser desorption ionizationtime of flight-mass spectrometry (MALDI -TOF-MS) has been used in the identification of catechin oligomers up to pentadecamer in apple (Guyot et al 1997) and up to nonamer in grape seeds (Yang and Chien 2000) however, these methods were not quantitative.…”

Section: Hplc and Hplc -Ms-proanthocyanidinsmentioning

confidence: 99%

Identification and Quantitation of Flavanols and Proanthocyanidins in Foods: How Good are the Datas?

Kelm¹,

Hammerstone²,

Schmitz

2005

Clinical and Developmental Immunology

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Evidence suggesting that dietary polyphenols, flavanols, and proanthocyanidins in particular offer significant cardiovascular health benefits is rapidly increasing. Accordingly, reliable and accurate methods are needed to provide qualitative and quantitative food composition data necessary for high quality epidemiological and clinical research. Measurements for flavonoids and proanthocyanidins have employed a range of analytical techniques, with various colorimetric assays still being popular for estimating total polyphenolic content in foods and other biological samples despite advances made with more sophisticated analyses. More crudely, estimations of polyphenol content as well as antioxidant activity are also reported with values relating to radical scavenging activity. High-performance liquid chromatography (HPLC) is the method of choice for quantitative analysis of individual polyphenols such as flavanols and proanthocyanidins. Qualitative information regarding proanthocyanidin structure has been determined by chemical methods such as thiolysis and by HPLC -mass spectrometry (MS) techniques at present. The lack of appropriate standards is the single most important factor that limits the aforementioned analyses. However, with ever expanding research in the arena of flavanols, proanthocyanidins, and health and the importance of their future inclusion in food composition databases, the need for standards becomes more critical. At present, sufficiently well-characterized standard material is available for selective flavanols and proanthocyanidins, and construction of at least a limited food composition database is feasible.

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Characterization of Grape Procyanidins Using High-Performance Liquid Chromatography/Mass Spectrometry and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

Cited by 104 publications

References 39 publications

Tannins from Canarium album with potent antioxidant activity

Tannins from Canarium album with potent antioxidant activity

Mass spectrometry in grape and wine chemistry. Part I: Polyphenols

Identification and Quantitation of Flavanols and Proanthocyanidins in Foods: How Good are the Datas?

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