Characterization of gold nanoparticles with different hydrophilic coatings via capillary electrophoresis and Taylor dispersion analysis. Part I: Determination of the zeta potential employing a modified analytic approximation

Pyell, Ute; Jalil, Alaa H.; Pfeiffer, Christian; Pelaz, Beatriz; Parak, Wolfgang J.

doi:10.1016/j.jcis.2015.03.006

Cited by 57 publications

(69 citation statements)

References 85 publications

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“…In this approach the velocity of NPs in an applied electric filed [52] is a parameter related to the mean NP charge [53,54], if other hydrodynamic features are not modified. As in capillary electrophoresis no matrix as in gel electrophoresis is used increase in hydrodynamic diameters has less effect than in case of gel electrophoresis, in which conjugates of biological molecules and NPs may be sorted predominantly by size [55][56][57][58].…”

Section: Description Of Experimental Techniquesmentioning

confidence: 99%

Dissociation coefficients of protein adsorption to nanoparticles as quantitative metrics for description of the protein corona: A comparison of experimental techniques and methodological relevance

Hühn

Fedeli

Zhang

et al. 2016

The International Journal of Biochemistry & Cell Biology

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Section: Description Of Experimental Techniquesmentioning

confidence: 99%

Dissociation coefficients of protein adsorption to nanoparticles as quantitative metrics for description of the protein corona: A comparison of experimental techniques and methodological relevance

Hühn

Fedeli

Zhang

et al. 2016

The International Journal of Biochemistry & Cell Biology

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“…Capillary electrophoresis has been used to measure zeta potential of charged nanoparticles including quantum dots [13], gold nanoparticles [14], latex particles [15], and single walled carbon nanotubes complexed with DNA [16]. While these two instrumental techniques are similar, detection with commercially available capillary electrophoresis instruments is typically limited to fluorescence or UV absorbance.…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis analysis of affinity to assess carboxylation of multi-walled carbon nanotubes

Davis

Patberg

Sargent

et al. 2018

Analytica Chimica Acta

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Surface oxidation improves the dispersion of carbon nanotubes in aqueous solutions and plays a key role in the development of biosensors, electrochemical detectors and polymer composites. Accurate characterization of the carbon nanotube surface is important because the development of these nano-based applications depends on the degree of functionalization, in particular the amount of carboxylation. Affinity capillary electrophoresis is used to characterize the oxidation of multi-walled carbon nanotubes. A polytryptophan peptide that contains a single arginine residue (WRWWWW) serves as a receptor in affinity capillary electrophoresis to assess the degree of carboxylation. The formation of peptide-nanotube receptor-ligand complex was detected with a UV absorbance detector. Apparent dissociation constants (K) are obtained by observing the migration shift of the WRWWWW peptide through background electrolyte at increasing concentrations of multi-walled carbon nanotubes. A 20% relative standard deviation in method reproducibility and repeatability is determined with triplicate analysis within a single sample preparation and across multiple sample preparations for a commercially available carbon nanotube. Affinity capillary electrophoresis is applied to assess differences in degree of carboxylation across two manufacturers and to analyze acid treated carbon nanotubes. The results of these studies are compared to X-ray photoelectron spectroscopy and zeta potential. Affinity capillary electrophoresis comparisons of carbon nanotube samples prepared by varying acid treatment time from 30 min to 3 h yielded significant differences in degree of carboxylation. X-ray photoelectron spectroscopy analysis was inconclusive due to potential acid contamination, while zeta potential showed no change based on surface charge. This work is significant to research involving carbon nanotube-based applications because it provides a new metric to rapidly characterize carbon nanotubes obtained from different vendors, or synthesized in laboratories using different procedures.

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“…The magnitude of the absolute value of the ζ‐potential indicates the degree of electrostatic repulsion of the individual particles to each other. Therefore, it is an important parameter to characterize and evaluate the colloidal stability of the synthesized nanoparticles . High ζ‐potential for small particles indicates an excellent colloidal stability due to the high electrostatic repulsion between the nanoparticles precluding their flocculation or aggregation.…”

Section: Characterization Of Functionalized Gnpsmentioning

confidence: 99%

Functionalized gold nanoparticles for sample preparation: A review

Liu

Lämmerhofer

2019

Electrophoresis

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Sample preparation is a crucial step for the reliable and accurate analysis of both small molecule and biopolymers which often involves processes such as isolation, pre‐concentration, removal of interferences (purification), and pre‐processing (e.g., enzymatic digestion) of targets from a complex matrix. Gold nanoparticle (GNP)‐assisted sample preparation and pre‐concentration has been extensively applied in many analytical procedures in recent years due to the favorable and unique properties of GNPs such as size‐controlled synthesis, large surface‐to‐volume ratio, surface inertness, straightforward surface modification, easy separation requiring minimal manipulation of samples. This review article primarily focuses on applications of GNPs in sample preparation, in particular for bioaffinity capture and biocatalysis. In addition, their most common synthesis, surface modification and characterization methods are briefly summarized. Proper surface modification for GNPs designed in accordance to their target application directly influence their functionalities, e.g., extraction efficiencies, and catalytic efficiencies. Characterization of GNPs after synthesis and modification is worthwhile for monitoring and controlling the fabrication process to ensure proper quality and functionality. Parameters such as morphology, colloidal stability, and physical/chemical properties can be assessed by methods such as surface plasmon resonance, dynamic light scattering, ζ‐potential determinations, transmission electron microscopy, Taylor dispersion analysis, and resonant mass measurement, among others. The accurate determination of the surface coverage appears to be also mandatory for the quality control of functionality of the nanoparticles. Some promising applications of (functionalized) GNPs for bioanalysis and sample preparation are described herein.

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Characterization of gold nanoparticles with different hydrophilic coatings via capillary electrophoresis and Taylor dispersion analysis. Part I: Determination of the zeta potential employing a modified analytic approximation

Cited by 57 publications

References 85 publications

Dissociation coefficients of protein adsorption to nanoparticles as quantitative metrics for description of the protein corona: A comparison of experimental techniques and methodological relevance

Dissociation coefficients of protein adsorption to nanoparticles as quantitative metrics for description of the protein corona: A comparison of experimental techniques and methodological relevance

Capillary electrophoresis analysis of affinity to assess carboxylation of multi-walled carbon nanotubes

Functionalized gold nanoparticles for sample preparation: A review

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