Characterization of Glomerella Strains Recovered from Anthracnose Lesions on Common Bean Plants in Brazil

Barcelos, Q.L.; Pinto, Joyce Mendes Andrade; Vaillancourt, Lisa J.; Souza, Elaine Aparecida de

doi:10.1371/journal.pone.0090910

Cited by 22 publications

(37 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…phaseoli, the sexual form of C. lindemuthianum, where the authors reported their ability to achieve sexual recombination as well the ability to form CAT. Moreover, they report the possibility of CAT formation between C. lindemuthianum and G. cingulata, increasing the pathogen variability (Barcelos, Pinto, Vaillancourt, & Souza, 2014). The genetic diversity of C. lindemuthianum was also reported by molecular markers using IRAP (Interretrotransposon Amplified Polymorphism) and Remap (retrotransposon Microsatellite), indicating high variability between groups of the pathogen and effectiveness in perform genetic diversity analysis (Santos et al, 2012;Gonzaga, Costa, Santos, Araujo, & Queiroz, 2014).…”

Section: Resultsmentioning

confidence: 93%

Classification of Colletotrichum lindemuthianum races in differential cultivars of common bean

et al. 2016

View full text Add to dashboard Cite

ABSTRACT. Anthracnose caused by the fungus Colletotrichum lindemuthianum is one of the main diseases affecting the common bean (Phaseolus vulgaris L.), and the pathogen is characterized by wide variability, with more than 50 physiological races identified in Brazil. Greater occurrences of races 65, 73, and 81 have been observed in Brazil along with the occurrence of pathogenic variability among isolates of a single race, destabilizing the resistance of commercial cultivars. Therefore, the aim of this study was to identify physiological races of C. lindemuthianum isolates collected in the states of São Paulo and Santa Catarina, Brazil and to test for variability among the isolates of race 65. The classification of 51 isolates resulted in the identification of 10 different physiological races: 4, 38, 55, 65, 73, 81, 83, 85, 321, and 351. Races 65 and 81 predominated, with frequencies of 37.25 and 35.29%, respectively. Regarding the isolates of race 65, wide physiological variability was evident, suggesting that a new differential set should be applied to detect the levels of variation among isolates of a single race of the pathogen.Keywords: physiological variability, levels of variation, anthracnose. Classificação de raças de Colletotrichum lindemuthianum em cultivares diferenciadoras de feijoeiro comum RESUMO.A antracnose causada pelo fungo Colletotrichum lindemuthianum é uma das principais doenças do feijoeiro comum (Phaseolus vulgaris L.), o patógeno caracteriza-se por apresentar uma grande variabilidade, com mais de 50 raças fisiológicas identificadas no Brasil. Tem-se evidenciado a maior ocorrência das raças 65, 73 e 81 no país e também a ocorrência de variabilidade patogênica entre isolados de uma mesma raça, que desestabilizam a resistência das cultivares comerciais. Portanto, o objetivo com este trabalho foi a identificação de raças fisiológicas de isolados de C. lindemuthianum coletados no Estado de São Paulo e Santa Catarina, Brasil e, detectar a ocorrência de variabilidade patogênica entre isolados pertencentes à raça 65. A classificação de 51 isolados resultou na identificação de 10 diferentes raças fisiológica: 4, 38, 55, 65, 73, 81, 83, 85, 321 e 351, com destaque para a raças 65 e 81, que apresentaram uma frequência de 37,25 e 35,29%, respectivamente. Com relação à avaliação entre os isolados da raça 65, ficou evidente a alta variabilidade dos mesmos, sugerindo a utilização de um novo conjunto diferenciador para detectar os níveis de variação entre isolados de uma mesma raça do patógeno.Palavras-chaves: variabilidade fisiológica, níveis de variação, antracnose.

show abstract

Section: Resultsmentioning

confidence: 93%

Classification of Colletotrichum lindemuthianum races in differential cultivars of common bean

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Ten strains showed the highest percentage of CATs, ranging from 7% (UFLA 43) to 16% (UFLA 39). The presence of CATs in species of Colletotrichum genus has been reported in common bean (Barcelos et al, ; Mota et al, ; Pinto et al, ) and apple (Araújo & Stadnik, ; Gonçalves & Stadnik, ).…”

Section: Discussionmentioning

confidence: 83%

“…(UFLA 39). The presence of CATs in species of Colletotrichum genus has been reported in common bean (Barcelos et al, 2014;Mota et al, 2016;Pinto et al, 2012) and apple (Araújo & Stadnik, 2013;Gonçalves & Stadnik, 2012).…”

Section: Understanding This Relationship Might Have Important Implicamentioning

confidence: 88%

See 1 more Smart Citation

Investigating variability and behaviour of Colletotrichum gloeosporioides strains from lesions of coffee blister spot

Pereira¹,

Souza²,

Pereira³

et al. 2019

Journal of Phytopathology

View full text Add to dashboard Cite

Coffee blister spot has been associated with species from the Colletotrichum genus, but there is no information on the variability of isolates present on leaf lesions. This study evaluated a population of Colletotrichum gloeosporioides strains from blister spot lesions in Coffea arabica. Colletotrichum spp. isolates were collected from blister spot lesions on leaves of coffee trees from Catuaí and Topázio cultivars (Coffea arabica). Monosporic cultures were obtained from colonies with sporulation. A pathogenicity test was carried out by inoculation of pathogens on the leaves of young coffee plants. C. gloeosporioides strains were characterized by morphologial, cytological and physiological analyses. The molecular analysis was carried out using Inter‐Retrotransposon Amplified Polymorphism (IRAP) markers. C. gloeosporioides strains showed no pathogenicity on coffee plants and presented a wide variability in all traits evaluated. The presence of sexual strains, formation of CATs (conidial anastomosis tubes) among conidial strains and high mycelial compatibility among strains observed suggest the occurrence of sexual and asexual recombination. The role of these C. gloeosporioides strains on the lesions of coffee plant leaves is unclear.

show abstract

“…Due to the necessity of fast obtaining sensitive and reliable results, molecular methods have arisen as promising alternatives for detecting C. lindemuthianum in bean seed lots, especially for routine analyses. The conventional PCR technique has already been used to diagnose anthracnose in bean plant tissues (Coêlho et al, 2016;Chen et al, 2007;Chen et al, 2013;Barcelos et al, 2014;García-Serrano et al, 2008;Wang et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Molecular detection of Colletotrichum lindemuthianum in bean seed samples

2018

View full text Add to dashboard Cite

Colletotrichum lindemuthianum is the causal agent of anthracnose in common bean, and infected seeds are the most typical propagation form of the disease. Thus, using common bean seeds free of C. lindemuthianum is crucial to managing this pest, as well as employing fast and accurate detection techniques to ensure high seed quality. In this study, both conventional and quantitative PCR techniques (cPCR and qPCR) were used for the detection and quantification of C. lindemuthianum in samples of common bean seeds. For that, seeds were inoculated by exposing them to fungal colonies for different periods of time, 0 h, 36 h, 72 h, 108 h and 144 h, each period corresponding to an inoculum potential. Then, they were mixed with healthy seeds, so incidences of 0.25%, 0.50%, 1%, 10%, and 100% of seeds with different inoculum potentials were obtained, in samples of 400 seeds. Both cPRC and qPCR techniques were effective in detecting the fungus. With the cPCR method, the highest sensitivity was recorded in those samples with 10% inoculated seeds with inoculum potential P36. On the other hand, with the qPCR technique, the highest sensitivity in detecting the fungus was observed in samples with 0.25% inoculated seeds with inoculum potential P36.Index terms: Phaseolus vulgaris, anthracnose, PCR.Detecção molecular de Colletotrichum lindemuthianum em amostras de sementes de feijão RESUMO -Colletotrichum lindemuthianum é o agente causal da antracnose do feijoeiro, tendo a semente como uma forma de disseminação. O uso de sementes de feijão livres do patógeno é uma das estratégias de manejo, e o uso de técnicas de detecção precisas e rápidas torna-se indispensável para a análise sanitária prévia de sementes. Neste estudo, as técnicas de PCR convencional (cPCR) e PCR em tempo real (qPCR) foram utilizadas para a detecção e quantificação de C. lindemuthianum em amostras de sementes de feijão. Para isso, sementes foram inoculadas por meio de sua exposição às colônias do fungo por tempos variados, 0, 36, 72, 108, e 144 horas, cada período correspondendo a um potencial de inóculo, P0, P36, P72, P108 e P144. As sementes inoculadas foram misturadas com sementes sadias, obtendo-se incidências de 0,25% 0,50%, 1%, 10% e 100% de sementes para cada potencial de inóculo em amostras de 400 sementes. Tanto a cPRC como a qPCR foram eficazes na detecção do fungo. A partir das amostras de sementes com 10% de incidência e com P36, foi possível detectar o fungo por cPCR. Pela técnica de qPCR o patógeno foi detectado em amostras a partir da incidência de 0,25% no menor potencial de inóculo P36.Termos para indexação: Phaseolus vulgaris, antracnose, PCR.1

show abstract

Characterization of Glomerella Strains Recovered from Anthracnose Lesions on Common Bean Plants in Brazil

Cited by 22 publications

References 65 publications

<b>Classification of <i>Colletotrichum lindemuthianum</i> races in differential cultivars of common bean

<b>Classification of <i>Colletotrichum lindemuthianum</i> races in differential cultivars of common bean

Investigating variability and behaviour of Colletotrichum gloeosporioides strains from lesions of coffee blister spot

Molecular detection of Colletotrichum lindemuthianum in bean seed samples

Contact Info

Product

Resources

About