Characterization of genipin-crosslinked gelatin/hyaluronic acid-based hydrogel membranes and loaded with hinokitiol: In vitro evaluation of antibacterial activity and biocompatibility

Effects of Hinokitiol and Dicalcium Phosphate on the Osteoconduction and Antibacterial Activity of Gelatin-Hyaluronic Acid Crosslinked Hydrogel Membrane In Vitro

“…Therefore, with the addition of modified DCPA, the ion–dipole physical bond between Ca 2+ and gelatin increases [ 24 ] ( Figure 1 b). However, hinokitiol with a hydroxyl and an isopropyl group leads to a large dipole moment [ 25 , 26 ]. Once hinokitiol is distributed in the hydrogel membrane, its hydrophobicity tends to cap the surface of hyaluronic acid instead of gelatin, and it interacts with Ca 2+ at the same time, thereby reducing the network structure of the ion–dipole physical bond between Ca 2+ and gelatin ( Figure 1 c).…”

“…Research has shown that the RGD sequence can promote cell adhesion in tissue engineering applications [ 25 , 26 ]. Resorbable bioceramics are largely incorporated into biodegradable polymer blends used in bone tissue engineering applications [ 29 ].…”

Hyaluronic Acid Hydrogels Crosslinked in Physiological Conditions: Synthesis and Biomedical Applications

“…The hydrogels were biocompatible and presented antibacterial activity against Escherichia coli . A similar approach was employed to form gelatin/HA crosslinked hydrogels involving genipin as the crosslinker [ 122 ]. Researchers also used HA to form polyelectrolyte complexes (PECs) based on chitosan/alginate/hyaluronic acid or collagen/chitosan/hyaluronic acid under mild conditions.…”

Hyaluronan as a Prominent Biomolecule with Numerous Applications in Medicine

“…Aminoethyl methacrylate HA Excellent swelling, mechanical property, low cytotoxicity, rapid hemostasis capacity and facilitated wound healing in mice [101] Alginate-HA fibers Good mechanical performance, high liquid absorption, and swelling percentage, high biocompatibility toward nHDF cell line, maintaining a moist wound surface [102] HA-poloxamer Promoted skin-wound healing and increased protein accumulation in the wound area in rats, higher air permeability than Band-aid [103] HA and lysozyme Hydrogel with suitable viscoelasticity and excellent adhesion on the skin tissue, low cytotoxicity [104] γ-Irradiated LMM HA High viability of L929 skin fibroblasts, faster wound healing after two days of healing in rats [105] HA with conjugated azobenzene and β-cyclo-dextrin groups Hydrogel with fast healing of skin wound in rats [106] • NO-releasing HA derivatives Effective against pathogens Staphylococcus aureus and Pseudomonas aeruginosa in skin wounds, enhanced wound closure and decreased quantity of the P. aeruginosa genetic material in the wound tissue in mice wounds [107] HA and cellulose High swelling capacity in various media [108] HA and hydroxyethyl cellulose Appropriate gelation time, good swelling ability, suitable water evaporation rate, well hemocompatibility, biological compatibility, super absorbent capacity [109] HA and chitosan Treatment of skin ulcers, decreased hydration properties of the dressing and modulation of drug release [82] There are numerous papers reporting preparations of wound dressings composed of three composite wound dressings involving HA biopolymer, e.g., HA, polycaprolactone and encapsulating growth factor [110], HA-povidone-iodine [111], HA, curcumin, pullulan [112], cornstarch/HA/propolis film [113], silk fibroin, HA, sodium alginate composite scaffold [114], HA gel, coenzyme Q10 or vitamin E, benzocaine [115], oxidized HA, vancomycin, adipic acid dihydrazide [116], HA, gelatin, genipin and hinokitiol [117], graphene oxide/copper nanoderivatives-modified chitosan/HA [118], calcium alginate hydrogel loaded with protamine nanoparticles and HA oligosaccharides [119], nanofibrous/gel structure of chitosan-gelatin/chitosan-HA [120], poly dimethylaminoethyl acrylate-HA with Didymocarpus pedicellatus extract hydrogel [121], HA, gelatin and chitosan scaffold [122], HA, silk fibroin, chitosan…”