Characterization of G10P[11] Rotaviruses Causing Acute Gastroenteritis in Neonates and Infants in Vellore, India

Iturriza‐Gómara, Miren; Kang, Gagandeep; Mammen, Ajit; Jana, Atanu Kumar; Abraham, Mutluri; Desselberger, Ulrich; Brown, David; Gray, Jim

doi:10.1128/jcm.42.6.2541-2547.2004

Cited by 150 publications

(129 citation statements)

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“…The G10P [11] genotype strain was an important genotype of group A BRV because of its zoonotic transmission from humans to cattle and also from cattle to humans, as reported by [6]. In this study, we describe the detection of bovine P [11], G10 genotype strains in Tamil Nadu which may be recognized as an important contributor to the diversity of rotaviruses found in human infections.…”

Section: Discussionmentioning

confidence: 89%

“…It is also important from the point of view of ecology and public health, because interspecies transmission from cattle to humans and from humans to cattle have been reported. Particularly rotavirus P [11], G10 strains, which are commonly found in cattle had frequently been associated with asymptomatic neonatal infections in Tamil Nadu, India [6].…”

Section: Introductionmentioning

confidence: 99%

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Genotyping of Rotavirus in Neonatal Calves with Acute Gastroenteritis in Iraq

Abdulazeez¹,

Abed²

2017

AiM

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Globally, Rotavirus is the common major etiologic agents of diarrhea in infant, young children and neonatal calves. It is very important to early diagnose the disease for effective treatment. The objective of this study was to determine the prevalence, molecular characteristics, and the effect of rotavirus strains for severe gastroenteritis in neonatal calves in five Iraqi governorates (Al-Qadissiya, Babel, Kerbala, Missan, Wassit). A total of 125-stool specimens were examined, it have been collected from calves form the period between November 2015 to March 2016. The ages were ranging from 6 to 60 weeks. The specimens were examined using Chromatographic Immunoassay, enzyme-linked immunosorbent assay (ELISA) and Polymerase-chain reaction (PCR). Our results gave us 67 (53.6%) positive by chromatographic immunoassay, 45 (36%) positive by ELISA and 32 (25.6%) positive by PCR. Genotyping were analyzed by multiplex PCR. Genotype combination G1P . Such information will not only aid in seeking advocacy for introducing rotavirus vaccine in national immunization program in Iraq, but will also help in the evaluation of the efficacy of these vaccines in relation to the rotavirus genotyping circulation.

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Section: Discussionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Genotyping of Rotavirus in Neonatal Calves with Acute Gastroenteritis in Iraq

Abdulazeez¹,

Abed²

2017

AiM

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“…Although rotaviruses are not entirely species specific (19,32), the vast majority of natural isolates obtained from any species, including humans, have been associated specifically with that species. Since the nonhuman primates most commonly used as experimental models are macaques, the goal was to obtain a challenge rotavirus strain that is endemic in macaques and fully adapted to infect this specific nonhuman primate.…”

Section: Discussionmentioning

confidence: 99%

Development of a Rotavirus-Shedding Model in Rhesus Macaques, Using a Homologous Wild-Type Rotavirus of a New P Genotype

McNeal

Sestak

Choi

et al. 2005

J Virol

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Although there are several reports on rotavirus inoculation of nonhuman primates, no reliable model exists. Therefore, this study was designed to develop a rhesus macaque model for rotavirus studies. The goals were to obtain a wild-type macaque rotavirus and evaluate it as a challenge virus for model studies. Once rotavirus was shown to be endemic within the macaque colony at the Tulane National Primate Research Center, stool specimens were collected from juvenile animals (2.6 to 5.9 months of age) without evidence of previous rotavirus infection and examined for rotavirus antigen. Six of 10 animals shed rotavirus during the 10-week collection period, and the electropherotypes of all isolates were identical to each other but distinct from those of prototype simian rotaviruses. These viruses were characterized as serotype G3 and subgroup 1, properties typical of many animal rotaviruses, including simian strains. Nucleotide sequence analysis of the VP4 gene was performed with a culture-grown isolate from the stool of one animal, designated the TUCH strain. Based on both genotypic and phylogenetic comparisons between TUCH VP4 and cognate proteins of representatives of the reported 22 P genotypes, the TUCH virus belongs to a new genotype, P[23]. A pool of wild-type TUCH was prepared and intragastrically administered to eight cesarean section-derived, specific-pathogen-free macaques 14 to 42 days of age. All animals were kept in a biocontainment level 2 facility. Although no diarrhea was observed and the animals remained clinically normal, all animals shed large quantities of rotavirus antigen in their feces after inoculation, which resolved by the end of the 14-day observation period. Therefore, TUCH infection of macaques provides a useful nonhuman primate model for studies on rotavirus protection.

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“…However, G6 strains have been detected in human cases in Italy [Gerna et al, 1992] and Australia [Cooney et al, 2001] and G8 strains have been detected in Indonesia [Matsuno et al, 1985], Finland [Gerna et al, 1990], Italy [Gerna et al, 1990], England [Steele et al, 1999], and Australia [Palombo et al, 2000]. Serotype G10 strains have been detected in human infections in Brazil [Santos et al, 1998], Paraguay [Coluchi et al, 2002], and India [Iturriza-Gómara et al, 2004]. In Africa, the prevalence of G8 strains may be as high as G3 and G4 strains in the rest of the world [Santos and Hoshino, 2005].…”

Section: Introductionmentioning

confidence: 99%

The detection and molecular characterization of human G12 genotypes in South Africa

Page

Beer

Seheri

et al. 2008

Journal of Medical Virology

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The last decade has seen an increase in the detection of rotavirus strains other than G1–G4 emerging or even predominating in some settings. The performance of the current rotavirus vaccines against unusual or rare circulating rotavirus serotypes cannot be predicted and continuous monitoring of wild type rotaviruses will remain a priority. Routine molecular rotavirus surveillance conducted in the Gauteng Province, South Africa during 2004, resulted in the detection of strains that could not typed using standard G specific genotyping primers. Sequencing of the first round amplicons revealed 19 serotype G12P[6] strains and one G12P[8] strain. Phylogenetic analyses of the G12 strains indicated that these strains are probably a recent introduction into South Africa and emerged from a strain related to the Indian isolate ISO‐5. The association of the South African G12s with the P[6] genotype may suggest a mechanism for unusual strains to become more ecologically suited to local population transmission dynamics. This is the first report of serotype G12 strains on the African continent and continued surveillance will be required to track the emergence of G12 strains in Africa. J. Med. Virol. 81:106–113, 2009. © 2008 Wiley‐Liss, Inc.

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Characterization of G10P[11] Rotaviruses Causing Acute Gastroenteritis in Neonates and Infants in Vellore, India

Cited by 150 publications

References 46 publications

Genotyping of Rotavirus in Neonatal Calves with Acute Gastroenteritis in Iraq

Genotyping of Rotavirus in Neonatal Calves with Acute Gastroenteritis in Iraq

Development of a Rotavirus-Shedding Model in Rhesus Macaques, Using a Homologous Wild-Type Rotavirus of a New P Genotype

The detection and molecular characterization of human G12 genotypes in South Africa

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