Characterization of Fusobacterium necrophorum subsp. necrophorum outer membrane proteins

Menon, Sailesh; Pillai, Deepti; Narayanan, Sanjeev

doi:10.1016/j.anaerobe.2018.01.015

Cited by 6 publications

(3 citation statements)

References 13 publications

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“…Porins possess a high proportion of β-sheet structure, which traverses the membrane in a tightly packed β-barrel organization. This is in agreement with the findings of [15] who successfully cloned and expressed the 42.4 kDa OMP (fom A gene) from F. necrophorum ssp. necrophorum in E. coli and showed that it increases the binding of E. coli to bovine adrenal gland capillary endothelial (EJG) cells, compared to the un-induced or control vector expressed in E. coli and concluded that the 42.4 kDa OMP serves as an adhesin for endothelial cells for bovine strains of F. necrophorum and may play an important role in host colonization and pathogenesis.…”

Section: Discussionsupporting

confidence: 92%

“…A 42.4 kDa outer membrane protein (FomA) of F. n ssp. necrophorum has been characterized and acts as an adhesin for strong binding to host cells, which is a key step in the disease pathogenesis [15]. However, at present there is no comprehensive information regarding the outer membrane proteins (OMPs) of F. necrophorum implicated in severe ovine footrot.…”

Section: Introductionmentioning

confidence: 99%

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Identification of Immunodominant Outer Membrane Proteins of Fusobacterium necrophorum from Severe Ovine Footrot By MALDI-TOF Mass Spectrometry

et al. 2021

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The aim of this study was to identify the immunodominant outer membrane proteins (OMPs) of Fusobacterium necrophorum from sheep affected with severe foot-rot. The OMP profile of ovine strains of F. necrophorum has not been well studied. We analyzed the OMP profile of the most frequent lktA variant JKS-F3 of F. necrophorum associated with severe ovine foot-rot with lesion score 4 in order to identify its major immunodominant OMPs. Electrophoretic separations of extracted OMPs showed a number of spots in two-dimensional electrophoretic gels. Two immunoreactive proteins of size around 43 kDa were identified through western blotting using hyperimmune sera raised in rabbits. These two immunogenic OMPs were analyzed by Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF/MS) which revealed that these two OMPs of lktA variant JKS-F3 of F. necrophorum showed 46 and 42 percent protein sequence coverage and scores of 125 and 114, respectively, with the reported 43 kDa outer membrane protein of F. necrophorum strain H05, a putative porin having properties similar to pore-forming proteins of anaerobic Gram-negative bacteria. These identified immunogenic OMPs will contribute to our understanding of the pathogenic role played by this organism in ovine foot-rot and could be exploited to devise an effective control strategy through development of an OMP-based recombinant vaccine to mitigate foot-rot in sheep and goats.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Identification of Immunodominant Outer Membrane Proteins of Fusobacterium necrophorum from Severe Ovine Footrot By MALDI-TOF Mass Spectrometry

et al. 2021

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“…Based on the N-terminal sequences, FomA protein in F. necrophorum has 96% homology with FomA of F. nucleatum [32]. This protein is immunogenic and plays a role in the attachment of bacteria to the host cells [33].…”

Section: Outer Membrane Proteins (Omp)mentioning

confidence: 99%

"Review on Fusobacterium necrophorum Putative Candidates for Vaccine Development Strategies: Outer Membrane Proteins and Outer Membrane Vesicles"

Narayanan¹

2022

BJSTR

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Fusobacterium necrophorum is a Gram-negative, strictly anaerobic bacterium associated with necrotic infections in animals and humans. The bacterium is an opportunistic and primary pathogen that causes liver abscess, footrot, and laryngeal infections in cattle. Liver abscess in cattle is reported at 20.7% annually, leading to liver condemnation and economic burden to the feedlot industry. Antibiotics are the mainstay for treatment; however, the reports of antibiotic resistance and demand for antibiotic-free, natural and organic beef have demanded alternative therapies and preventatives. Hence, developing an effective vaccine is essential to control infections and economic loss to the cattle industry. Currently, there is no licensed vaccine to prevent liver abscesses in cattle. A number of virulence factors for F. necrophorum have been explored in the past for vaccine development. Each one has some advantages and disadvantages concerning immunogenicity and protective effect. The review summarizes vaccine candidates explored in the past, mainly focusing on F. necrophorum. The review also connects some concepts related to virulence factors found in F. necrophorum and how it could be a promising vaccine candidate based on the studies done in other Gram-negatives. Mini ReviewFusobacterium necrophorum, a Gram-negative anaerobic bacillus, is an opportunistic pathogen isolated from oral cavities, gastrointestinal and genitourinary tracts of humans and animals [1,2]. The bacterium is associated with Lemierre's syndrome affecting young and healthy individuals and necrotic infections in hepatic, abdominal, and respiratory organs in animals [3,4]. The bacteria are primary causative agents of liver abscess, foot rot, and calf diphtheria, sometimes in mixed infections with other bacteria such as Trueperella pyogenes and Porphyromonas species [5,6]. F. necrophorum has been classified into four biotypes: A, B, AB, and C [7]. Biotypes A and B are of veterinary importance and associated with cattle liver abscesses. Biotype A, subspecies necrophorum, and Biotype B, subspecies funduliforme, vary in cell morphology, colony characteristics, virulence capacity, virulence factors, 16s rRNA sequences, and DNA gyrase B subunit [7,8]. The subspecies necrophorum is more virulent and frequently isolated than the subspecies funduliforme, and is the primary causative agent in liver abscesses [9]. Annually, the rate of incidence of liver abscess in feedlot cattle is 10-20% [10].Generally, the incidence is higher in cattle fed with high grainbased diets where the progression occurs from chronic acidosis and rumenitis to liver abscess [11]. The National Beef Quality

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Gram‐Negative Anaerobes

Prescott¹

2022

Pathogenesis of Bacterial Infections in Animals

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Characterization of Fusobacterium necrophorum subsp. necrophorum outer membrane proteins

Cited by 6 publications

References 13 publications

Identification of Immunodominant Outer Membrane Proteins of Fusobacterium necrophorum from Severe Ovine Footrot By MALDI-TOF Mass Spectrometry

Identification of Immunodominant Outer Membrane Proteins of Fusobacterium necrophorum from Severe Ovine Footrot By MALDI-TOF Mass Spectrometry

"Review on Fusobacterium necrophorum Putative Candidates for Vaccine Development Strategies: Outer Membrane Proteins and Outer Membrane Vesicles"

Gram‐Negative Anaerobes

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