Characterization of fruit trees pollen

Pospiech, Matěj; Javůrková, Zdeňka; Tremlová, Bohuslava; Běhalová, Hana

doi:10.5219/1096

Cited by 4 publications

(5 citation statements)

References 28 publications

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“…Our results confirmed that these descriptors can be used for classification and they show slight differences between individual microscopic methods. Feret diameter is also confirmed in literature as a relevant descriptor for pollen classification by DF [ 51 ] and by BF [ 5 ]. P/E ratio is commonly used for pollen description, where P stands for polar diameter and E stands for equatorial diameter.…”

Section: Resultsmentioning

confidence: 53%

“…Shape characteristics are used to describe the geometric parameters of an object and are determined by a simplified mathematical model. For pollen grain discrimination, they represent an important parameter, both, for human evaluation [ 33 ] as well as for automatic systems [ 5 , 19 ].…”

Section: Resultsmentioning

confidence: 99%

“…In comparison with other authors, for Ph, the Brassica pollen length was 24.48 μm, Prunus pollen length was 36.94 μm, Asteraceae represents a wide group the pollens sized within the range of 33.16–195.08 [ 41 ]. For BF, the size of Brassica was measured at 21–30 μm, for Salix 16–27 μm, for Asteraceae (single species Helianthus ) 27–31 μm [ 19 ], for the Prunus 55.15 μm [ 5 ]. On the other hand, size and morphological similarities were likely related to the confusion in taxa classification for DF [ 24 ].…”

Section: Resultsmentioning

confidence: 99%

“…A number of different microscopic techniques are used for pollen identification. Morphological characteristics [ 3 ], especially the shape characteristics (length, width, circularity, and shape factor or length/width ratio) [ 4 , 5 ], are used most commonly. In light microscopy (LM) the morphological (pollen shape) and surface structures (exina) are used for the identification as well [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

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Identification of pollen taxa by different microscopy techniques

et al. 2021

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Melissopalynology is an important analytical method to identify botanical origin of honey. Pollen grain recognition is commonly performed by visual inspection by a trained person. An alternative method for visual inspection is automated pollen analysis based on the image analysis technique. Image analysis transfers visual information to mathematical descriptions. In this work, the suitability of three microscopic techniques for automatic analysis of pollen grains was studied. 2D and 3D morphological characteristics, textural and colour features, and extended depth of focus characteristics were used for the pollen discrimination. In this study, 7 botanical taxa and a total of 2482 pollen grains were evaluated. The highest correct classification rate of 93.05% was achieved using the phase contrast microscopy, followed by the dark field microscopy reaching 91.02%, and finally by the light field microscopy reaching 88.88%. The most significant discriminant characteristics were morphological (2D and 3D) and colour characteristics. Our results confirm the potential of using automatic pollen analysis to discriminate pollen taxa in honey. This work provides the basis for further research where the taxa dataset will be increased, and new descriptors will be studied.

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Section: Resultsmentioning

confidence: 53%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification of pollen taxa by different microscopy techniques

et al. 2021

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“…Previously, the pollen of some varieties of M. domestica Borkhausen was identified by some authors in America (Fogle, 1977a, b), New Zealand (Currie et al, 1997), Iran (Joneghani, 2008), Slovakia (Motyleva et al, 2017), Russia (Motyleva et al, 2018), Czech Republic (Pospiech et al, 2019).…”

Section: Quantitativementioning

confidence: 99%

Study of Phenotypes Variability of Pollen Grains Malus domestica Borkh. by Scanning Electron Microscopy

Sedláčková¹

2021

Agrobiodivers Improv Nutr health life Qual

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The studying of Malus domestica Borkh. pollen allows us to determine the details of morphological characteristics and describe the most important parameters and pollen sculpture that can be used to identify representatives of species. We analysed pollen morphology of seven Malus domestica genotypes from old and local varieties from the territory of Slovakia via a scanning electron microscope. We used principal components analysis to explore variability in pollen grain size (polar and equatorial diameter), shape, aperture type, and exine ornamentation. The complexity of these morphological characteristics and ultrastructure allows determining the differences or similarities between the same and various species and genotypes, which may be a useful tool for systematics with significant diagnostic value. Findings confirmed small differences among the genotypes in measured traits with polar and equatorial diameters in the range from 31.85 to 42.85 μm and from 21.23 to 23.93 μm, respectively. Shape index (P/E ratio) depending on elongation or roundness of pollen grains varied from 1.44 to 1.87. Hierarchical cluster analysis and principal component analysis of morphological data helped to compare evaluated morphometric parameters and identified five closely related groups. It was noted that diversity of surface sculpturing of pollen grains in combination with shape and size enables to use of a complex of thin morphologic signs for M. domestica pollen identifications. Pollen data combined with other morphological evidence (e.g., floral characters) have more recently become an important indicator of which genotypes may be the best representatives of species.

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Pollen diversity in honeys of the Czech Republic in the 2019 season

Pospiech

Ljasovská

Titěra

et al. 2020

Potr. S. J. F. Sci.

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Honeybees are important pollinators. As a side product of pollination, honeybees produce honey, as a natural sweetener. The source of honey depends on the hive location. In specific conditions honeybees produce monofloral honey, but more common are polyfloral kinds of honey. In this study honey from the Czech Republic in the 2019 season was evaluated by melissopalynology analysis. The common botanical taxa in the Czech Republic were determined and season impact to pollen taxa was compared for dominant pollen taxa. The taxonomic distribution of pollen in Czech honey was stable during the year. The average number of species was 11.52 taxa per sample. The dominant pollen source in Czech honey was the Brassicaceae family. The high pollen content in honey was confirmed also in the Rosacea family (fruit tree), Ubelliferacae family and Myosotis genus. During the year the pollen taxa were equally distributed in honey. Seasonal effects were confirmed only in Salix genus, Ubelliferacae family and Phacelia genus. Seasonal effects correspond with the blooming season and honeybee handling in the hive was also confirmed. High variability during the season and hive location was confirmed for other taxa.

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Characterization of fruit trees pollen

Cited by 4 publications

References 28 publications

Identification of pollen taxa by different microscopy techniques

Identification of pollen taxa by different microscopy techniques

Study of Phenotypes Variability of Pollen Grains Malus domestica Borkh. by Scanning Electron Microscopy

Pollen diversity in honeys of the Czech Republic in the 2019 season

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