Characterization of foot-and-mouth disease virus from outbreaks in Ecuador during 2009–2010 and cross-protection studies with the vaccine strain in use in the region

Maradei, Eduardo; Beascoechea, Claudia Perez; Malirat, Viviana; Salgado, Gustavo; Seki, Cristina; Pedemonte, Andrea; Bonastre, Paula; D’Aloia, Ricardo; Torre, J.L. La; Mattion, Nora; Toledo, Jorge Rodríguez; Bergmann, I

doi:10.1016/j.vaccine.2011.08.120

Cited by 27 publications

(22 citation statements)

References 36 publications

(60 reference statements)

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“…The mutated binding sites identified by the mAbs for ECU/4/10 are located on the antigenic sites 1a, 1b, 2, 4 and one on non-neutralizing site. This result of high antigenic variation is consistent with previously reported data for the ECU/4/10 isolates [28]. Viruses circulating in Ecuador during the years 2009–2010 were examined using monoclonal antibody profiling.…”

Section: Discussionsupporting

confidence: 90%

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Characterization of monoclonal antibodies against foot-and-mouth disease virus serotype O and application in identification of antigenic variation in relation to vaccine strain selection

Yang

Goolia

et al. 2014

Virol J

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BackgroundFoot-and-mouth disease (FMD) has severe implications for animal farming which leads to considerable financial losses because of its rapid spread, high morbidity and loss of productivity. For these reasons, the use of vaccine is often favoured to prevent and control FMD. Selection of the proper vaccine is extremely difficult because of the antigenic variation within FMDV serotypes. The aim of the current study was to produce a panel of mAbs and use it for the characterization of new isolates of FMDV serotype O.ResultsA panel of FMDV/O specific mAb was produced. The generated mAbs were then characterized using the peptide array and mAb resistant mutant selection. Seven out of the nine mAbs reacted with five known antigenic sites, thus the other two mAbs against non-neutralizing sites were identified. The mAbs were then evaluated by antigenic ELISA for the detection of forty-six FMDV serotype O isolates representing seven of ten known topotypes. Isolates ECU/4/10 and HKN/2/11 demonstrated the highest antigenic variation compared to the others. Furthermore, the panel of mAbs was used in vaccine matching by antigenic profiling ELISA with O1/Manisa as the reference strain. However, there was no correlation between vaccine matching by antigenic ELISA and the gold standard method, virus neutralisation test (VNT), for the forty-six FMDV/O isolates. Nine isolates had particularly poor correlation with the reference vaccine strain as revealed by the low r1 values in VNT. The amino acid sequences of the outer capsid proteins for these nine isolates were analyzed and compared with the vaccine strain O1/Manisa. The isolate ECU/4/10 displayed three unique amino acid substitutions around the antigenic sites 1, 3 and 4.ConclusionsThe panel of mAbs is useful to monitor the emergence of antigenically different strains and determination of relevant antigenic site differences. However, for vaccine matching VNT remains the preferred method but a combination of VNT, antigenic profiling with a panel of mAbs and genetic sequencing would probably be more ideal for full characterization of any new outbreak isolates as well as for selection of vaccine strains from FMDV antigen banks.

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Section: Discussionsupporting

confidence: 90%

“…The results showed that the viruses lost reactivity with the four mAbs, three of them with neutralizing properties. Moreover, results obtained with in vivo challenge indicated a lack of protection by the vaccine virus (O1/Campos) [28]. …”

Section: Discussionmentioning

confidence: 99%

Characterization of monoclonal antibodies against foot-and-mouth disease virus serotype O and application in identification of antigenic variation in relation to vaccine strain selection

Yang

Goolia

et al. 2014

Virol J

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“…In these studies the challenge was with a homologous virus. Protection against heterologous challenge has been shown for serotypes A in cattle [8] but similar studies with serotype O did not yield satisfactory results [9,10].…”

Section: Introductionmentioning

confidence: 98%

Efficacy of a high potency O1 Manisa monovalent vaccine against heterologous challenge with a FMDV O Mya98 lineage virus in pigs 4 and 7 days post vaccination

Vosloo

Hong²,

Fosgate

et al. 2015

Vaccine

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Early protection with a high potency (>6PD50) foot-and-mouth disease (FMD) O1 Manisa (Middle-East South Asia lineage) vaccine against challenge with O/VIT/2010 (O Mya98 lineage) was tested in pigs. Only two pigs that were vaccinated seven days prior to challenge had any demonstrable antibodies as a result of vaccination at the time of challenge. However, 80% and 60% of pigs that were vaccinated seven and four days prior to coronary band challenge were protected. Vaccination significantly reduced the amount of virus excreted in nasal swabs, saliva and faeces compared to unvaccinated and infected controls. Virus and viral RNA could be detected in some pigs until termination of the experiment 14 days after challenge. Antibodies to the non-structural proteins (NSP) were detected in only one pig that was challenged four days post vaccination (dpv) and transiently in two pigs that were challenged sevendpv at only one time point. For each vaccine and control group, a group of unvaccinated pigs were kept in the same room but with no direct contact with the infected pigs to determine whether vaccination prevented transmission. Despite the presence of live virus and viral RNA in these indirect contact pigs, the groups in contact with the vaccinated and infected pigs did not develop clinical signs nor did they sero-convert. Contact pigs in the same room as unvaccinated challenged controls did show signs of disease and virus infection that resulted in sero-conversion to the NSP. A breach of the wall that separated the two groups at nine days post challenge might have contributed to this finding. This study showed that high potency vaccine can provide protection to pigs soon after vaccination and that aerosol transmission within rooms is a rare event.

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“…However, a single test is specific for only one serotype. The strip test for serotype O cannot detect the isolate, ECU4/10, which was isolated in Ecuador in 2009 and 2010 (Maradei et al, 2011). Amada et al (2014) developed a multiplex strip test for the serological survey of hantavirus infections in humans, but there was no test available for multiplex FMDV detection using a single LFI strip test, until now.…”

Section: Introductionmentioning

confidence: 99%

Development of a multiplex lateral flow strip test for foot-and-mouth disease virus detection using monoclonal antibodies

Yang

Caterer²,

et al. 2015

Journal of Virological Methods

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Foot-and-mouth disease (FMD) is one of the world's most highly contagious animal diseases with tremendous economic consequences. A rapid and specific test for FMD diagnosis at the site of a suspected outbreak is crucial for the implementation of control measures. This project developed a multiplex lateral flow immunochromatographic strip test (multiplex-LFI) for the rapid detection and serotyping of FMD viruses. The monoclonal antibodies (mAbs) against serotypes O, A, and Asia 1 were used as capture mAbs. The mAbs were conjugated with fluorescein, rhodamine or biotin for serotype O, A and Asia 1, respectively. The detection mAbs which consisted of a serotype-independent mAb in combination with one serotype A-specific mAb and one Asia 1-specific mAb, were each colloidal gold-conjugated. The strips used in this study contained one control line and three test lines, which corresponded to one of the three serotypes, O, A or Asia 1. The newly developed multiplex-LFI strip test specifically identified serotype O (n=46), A (n=45) and Asia 1 (n=17) in all tested field isolates. The sensitivity of this strip test was comparable to the double antibody sandwich ELISA for serotypes O and A, but lower than the ELISA for serotype Asia 1. The multiplex-LFI strip test identified all tissue suspensions from animals that were experimentally inoculated with serotypes O, A or Asia 1. FMD viruses were detected in 38% and 50% of the swab samples from the lesion areas of experimentally inoculated sheep for serotypes O and A, respectively. The capability of the multiplex-LFI strip tests to produce rapid results with high specificity for FMD viruses of multiple serotypes makes this test a valuable tool to detect FMD viruses at outbreak sites.

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Characterization of foot-and-mouth disease virus from outbreaks in Ecuador during 2009–2010 and cross-protection studies with the vaccine strain in use in the region

Cited by 27 publications

References 36 publications

Characterization of monoclonal antibodies against foot-and-mouth disease virus serotype O and application in identification of antigenic variation in relation to vaccine strain selection

Characterization of monoclonal antibodies against foot-and-mouth disease virus serotype O and application in identification of antigenic variation in relation to vaccine strain selection

Efficacy of a high potency O1 Manisa monovalent vaccine against heterologous challenge with a FMDV O Mya98 lineage virus in pigs 4 and 7 days post vaccination

Development of a multiplex lateral flow strip test for foot-and-mouth disease virus detection using monoclonal antibodies

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