Characterization of feline serum–cobalt binding

Abstract

The correlation of cobalt binding measured by a colorimetric test with albumin concentration in the clinical samples and with serum dilution is consistent with feline albumin-cobalt complex formation. Hypoalbuminemia is the likely cause of reduced serum-cobalt binding in inflammation and the correlations observed between cobalt binding and other variables.

Specificity of, and influence of hemolysis, lipemia, and icterus on serum lipase activity as measured by the v‐LIP‐P slide

“…However, it should be noted that Intralipid is not a physiologic serum triglyceride. While Intralipid has been used as a standard spiking substance for assessing the interference due to lipemia with serum biochemical assays for many years, there is a potential that lipemia associated with spontaneous hypertriglyceridemia has less of an influence on serum lipase activity than does the addition of Intralipid to the sample …”

“…While Intralipid has been used as a standard spiking substance for assessing the interference due to lipemia with serum biochemical assays for many years, there is a potential that lipemia associated with spontaneous hypertriglyceridemia has less of an influence on serum lipase activity than does the addition of Intralipid to the sample. [10][11][12][13] Thus, in order to evaluate whether spontaneous hypertriglyceridemia had a comparable effect on the measurement of serum lipase activity using the v-LIP-P slide, the third part of the study measured lipase activity in 8 dogs with naturally occurring hypertriglyceridemia. While 5 of 8 hypertriglyceridemic serum samples showed serum lipase activities above the upper limit of the RI, none of them reached the upper detection limit of the assay as had been the case for samples spiked with Intralipid.…”