Specificity of, and influence of hemolysis, lipemia, and icterus on serum lipase activity as measured by the v‐<scp>LIP</scp>‐P slide

Steiner, Jöerg M.; Gomez, Robynne R; Suchodolski, Jan S.; Lidbury, Jonathan A.

doi:10.1111/vcp.12517

Cited by 7 publications

(16 citation statements)

References 11 publications

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“…Pancreatic lipase immunoreactivity concentrations, as measured using Spec fPL and Spec cPL, are currently considered the most sensitive and specific laboratory tests for feline and canine pancreatitis, depending on the diagnostic criteria of pancreatitis and cutoffs used 12,15,17,34‐37 . Various studies describing the analytical validation of the Spec cPL assay have shown this assay to be precise, linear, stable, reproducible, and unaffected by hemolysis, lipemia, or bilirubinemia 9,38 . In this study, we further showed that extrapancreatic lipases (ie, heparin‐induced lipases, hepatic and lipoprotein lipases) do not interfere with Spec PL measurements.…”

Section: Discussionsupporting

confidence: 58%

The 1,2‐o‐dilauryl‐rac‐glycero‐3‐glutaric acid‐(6’‐methylresorufin) ester (DGGR) lipase assay in cats and dogs is not specific for pancreatic lipase

Lim

Xenoulis

Stavroulaki

et al. 2020

Veterinary Clinical Pathol

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BackgroundThe measurement of pancreatic lipase is important for the diagnosis of feline and canine pancreatitis. Recent studies have claimed that lipase assays using the 1,2‐o‐dilauryl‐rac‐glycero‐3‐glutaric acid‐(6’‐methylresorufin) ester (DGGR) as a substrate are more specific for measuring pancreatic lipase than traditional lipase assays. However, the analytical specificity of this assay for pancreatic lipase has not been demonstrated.ObjectivesWe aimed to determine whether hepatic and/or lipoprotein lipases can interfere with the DGGR‐based assay results in cats and dogs. We, therefore, compared plasma lipase activities measured using DGGR‐based and pancreatic lipase immunoreactivity (PLI) assays before and after administering heparin, known to cause the release of hepatic and lipoprotein lipases, in cats and dogs.MethodsHeparin was administered in six cats and six dogs. Blood was collected at baseline and 10, 20, 30, 60, and 120 minutes after heparin administration. Lipase activity was measured using a DGGR‐based assay, and PLI concentrations were measured using the Spec fPL and cPL assays for cats and dogs, respectively.ResultsPlasma lipase activities, as measured using the DGGR‐based assay, increased significantly 10 minutes after heparin administration in both cats (P = .003) and dogs (P = .006) and returned to baseline by 120 minutes. In contrast, PLI concentrations showed no significant changes after heparin administration.ConclusionsDGGR is not only hydrolyzed by pancreatic lipase but also by hepatic lipase, lipoprotein lipase, or both, in cats and dogs. Since these extrapancreatic lipases are also naturally present in cats and dogs, they could contribute to the lack of analytical specificity for the DGGR‐based assays.

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Section: Discussionsupporting

confidence: 58%

The 1,2‐o‐dilauryl‐rac‐glycero‐3‐glutaric acid‐(6’‐methylresorufin) ester (DGGR) lipase assay in cats and dogs is not specific for pancreatic lipase

Lim

Xenoulis

Stavroulaki

et al. 2020

Veterinary Clinical Pathol

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“…However, overlap existed and 42% of dogs had lipase concentrations above the lower 20% of the reference interval, indicating that many dogs had substantial detection of lipase by the V‐LIP‐P (Fujifilm Corporation) assay. Thus, triolein does not appear to be specific for the measurement of lipase of pancreatic origin 78 …”

Section: Lipase Assaysmentioning

confidence: 96%

“…The standard for such studies uses the addition of Intralipid to serum, which has been reported to falsely amplify the effects of lipids on lipase activity assays. In 8 dogs with naturally‐occurring hyperlipidemia, the effects of lipid on V‐LIP‐P (Fujifilm Corporation) results were lower 78 . This study also evaluated V‐LIP‐P (Fujifilm Corporation) lipase concentrations in 50 dogs with EPI.…”

Section: Lipase Assaysmentioning

confidence: 97%

“…In 8 dogs with naturally‐occurring hyperlipidemia, the effects of lipid on V‐LIP‐P (Fujifilm Corporation) results were lower. 78 This study also evaluated V‐LIP‐P (Fujifilm Corporation) lipase concentrations in 50 dogs with EPI. The study found significant differences between lipase concentrations measured by the V‐LIP‐P (Fujifilm Corporation) assay when compared to healthy dogs.…”

Section: Lipase Assaysmentioning

confidence: 99%

“…Thus, triolein does not appear to be specific for the measurement of lipase of pancreatic origin. 78 …”

Section: Lipase Assaysmentioning

confidence: 99%

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Advances in the diagnosis of acute pancreatitis in dogs

Cridge

Twedt

Marolf

et al. 2021

Veterinary Internal Medicne

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In the last 20 years, the diagnosis of pancreatitis has become more frequent as a result of improved diagnostic modalities such as abdominal ultrasound examination, advanced imaging, and immunoassays for the measurement of pancreatic lipase. Our aim is to provide a state‐of‐the‐art overview of the clinical diagnosis of acute pancreatitis (AP) in dogs with a particular focus on pancreatic lipase assay validation and clinical performance, in addition to advanced imaging modalities. We also discuss the potential indications for cytology and histopathology in dogs with suspected AP.

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Laboratory Diagnosis of Pancreatitis

Rudinsky¹

2023

Veterinary Clinics of North America: Small Animal Practice

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Specificity of, and influence of hemolysis, lipemia, and icterus on serum lipase activity as measured by the v‐LIP‐P slide

Cited by 7 publications

References 11 publications

The 1,2‐o‐dilauryl‐rac‐glycero‐3‐glutaric acid‐(6’‐methylresorufin) ester (DGGR) lipase assay in cats and dogs is not specific for pancreatic lipase

The 1,2‐o‐dilauryl‐rac‐glycero‐3‐glutaric acid‐(6’‐methylresorufin) ester (DGGR) lipase assay in cats and dogs is not specific for pancreatic lipase

Advances in the diagnosis of acute pancreatitis in dogs

Laboratory Diagnosis of Pancreatitis

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