Characterization of extremely halophilic Archaea isolated from saline environment in different parts of Turkey

Özcan, Birgül; Çökmüş, Cumhur; Coleri, Arzu; Çalışkan, Mahmut

doi:10.1134/s002626170606018x

Cited by 15 publications

(11 citation statements)

References 27 publications

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“…Archaeal strains, growth conditions and screening for lipolytic activity A total of 118 halophilic archaeal collection of strains isolated from Salt Lake (Ankara), Aci Lake (Denizli) and Tuzla Lake (Kayseri) located in Turkey were routinely cultured in Sehgal-Gibbons (SG) medium, which contains the following (g/l): NaCl, 250; MgSO 4 ·7H 2 O, 20; KCl, 2; sodium citrate (trisodium salt), 3; casamino acids, 7.5; yeast extract, 1; FeSO 4 ·7H 2 O, 0.0023 [19]. Agar plates contained 2% (w/v) agar.…”

Section: Methodsmentioning

confidence: 99%

“…Recently, it was published that Lakes of Turkey are rich sources of halophilic archaeal microorganisms [19] and some of these strains were characterized in a phylogenetic manner [20]. A screening method performed for the isolation of extracellular enzyme producers will provide the possibility to use diVerent halophiles as a source of extremophilic enzymes in biotechnological processes.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of extracellular esterase and lipase activities from five halophilic archaeal strains

Özcan¹,

Özyılmaz²,

Çökmüş³

et al. 2008

J Ind Microbiol Biotechnol

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A total of 118 halophilic archaeal collection of strains were screened for lipolytic activity and 18 of them were found positive on Rhodamine agar plates. The selected five isolates were further characterized to determine their optimum esterase and lipase activities at various ranges of salt, temperature and pH. The esterase and lipase activities were determined by the hydrolysis of pNPB and pNPP, respectively. The maximum hydrolytic activities were found in the supernatants of the isolates grown at complex medium with 25% NaCl and 1% gum Arabic. The highest esterase activity was obtained at pH 8-8.5, temperature 60-65 degrees C and NaCl 3-4.5 M. The same parameters for the highest lipase activities were found to be pH 8, temperature 45-65 degrees C and NaCl 3.5-4 M. These results indicate the presence of salt-dependent and temperature-tolerant lipolytic enzymes from halophilic archaeal strains. Kinetic parameters were determined according to Lineweaver-Burk plot. The KM and V (max) values were lower for pNPP hydrolysis than those for pNPB hydrolysis. The results point that the isolates have higher esterase activity comparing to lipase activity.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of extracellular esterase and lipase activities from five halophilic archaeal strains

Özcan¹,

Özyılmaz²,

Çökmüş³

et al. 2008

J Ind Microbiol Biotechnol

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“…They showed high rate of amylase production but neither protease nor any lipase activity were observed. Ozcan et al (2006) and Birbir et al (2007) showed amylase, protease, lipase and amylase, protease, lipase cellulase, nuclease activity in the haloarchaeal strains, respectively. Anikandan et al (2009) could detect amylase, protease, Haloarcula,Natrinema,Halovivax and Natronomonas.…”

Section: Characterization Of the Isolatesmentioning

confidence: 98%

Diversity of hydrolytic enzymes in haloarchaeal strains isolated from salt lake

Makhdoumi

Amoozegar

Khaledi

2011

Int. J. Environ. Sci. Technol.

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ABSTRACT:Production of ten hydrolytic enzymes was qualitatively studied on the haloarchaeal strains isolated from Aran-Bidgol hypersaline lake in the central desert area of Iran. A total of 293 haloarchea strains were selected among 300 extremely halophilic isolated prokaryotes. Accordingly, 142, 141, 128, 64, 38, 16, 7, 3 and 1 archaeal isolates were able to produce DNase, amylase, lipase, inulinase, pullulanase, protease, cellulase, chitinase and xylanase, respectively. None was able to produce pectinase activity. Combined hydrolytic activity was also detected in many strains. A total of 0.3 % of the strains showed 6 hydrolytic activities, 0.3 % of the strains had 5 hydrolytic activities, 5.4 % of the strains presented 4 hydrolytic activities, 25 % of the strains presented 3 hydrolytic activities, 28 % of the strains presented 2 hydrolytic activities and 18 % of the strains presented 1 hydrolytic activity. According to their phenotypic characteristics and comparative partial 16 S rRNA sequence analysis, the halophilic strains were all identified as members of family Halobacteriaceae within 12 different taxa from the following genera: Halorubrum, Haloarcula, Natrinema, Halovivax and Natronomonas. Most enzymes production rate was observed in the genera Halorubrum, Haloarcula and Natrinema whereas; there was not any detectable amount of enzyme production in the genera Halovivax and Natronomonas. The most hydrolytic isolate with 6 combinatorial enzyme production belonged to the genus Natrinema. This investigation showed that the extreme halophilic archaea from Aran-Bidgol lake are a potential source of hydrolytic enzyme under stress conditions and may have possess commercial value.

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“…Moreover, protease enzyme was the most common enzyme of screened strains (represented 39.4%), and produced by species with various genera, Halobiforma, Natronococcus, Natronobacterium , Natrinema, Natrialba, Natronomonas and Haloterrigena. Ozcan et al (2006) demonstrated the production of amylase, protease, lipase from haloarchaeal strains isolated from hypersaline environment, while Birbir et al (2007) showed amylase, protease, lipase, cellulase, nuclease activities in the same environment.…”

Section: Discussionmentioning

confidence: 99%

Screening of Hydrolytic Extremozymes in Haloalkaliphilic Archaea by Culture and Molecular-based Methods

Hamedo¹,

Hagagy²,

Shafi³

et al. 2017

Egypt. J. Exp. Biol. (Bot.)

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Screening of hy droly tic extremoz y mes in haloalkali ph ilic Archaea by culture and molec ular -bas ed m ethods ABSTRACT:Exploring of extrem ophilic Archaea and their enzym es had great significance to biocatalysis. Enzym es produced by Archaea allow im provem ent in m ultiple sectors of industry. They can help reduce the quantity of w aste energy and material consumption, thus m aking the technology m ore environm entally-friendly. This study aim ed to screen hydrolytic extrem ozym es in different Soda Lakes of W adi Al-Natrun, Egypt, by enzym atic agar -plate assays and m olecular-based m ethods. Five hundred and thirty-five haloalkaliphilic archaeal strains isolated from different Soda Lakes w ere screened for production of protease, am ylase, pectinase, chitinase, cellulase, lipase and esterase at pH 10 and 25% NaCl (w /v). Furtherm ore, m etagenomic DNA w as extracted from w ater sam ple of Ga'ar Lake and constructed library w ere sequenced to identify the genes encoding target enzymes by using illum ina Hiseq2000 system . By enzym atic agarplate assay, all tested strains show ed potential production of extracellular enzym es, a total of 39.4% of screened strains produced protease, 27.1% show ed am ylase activity, 25.9% for lipase and 7.4% displayed cellulase activity, but none of tested strains produced chitinase or pectinase. W hile, by shotgun m etagenomic technique, all genes encoding m etabolically active hydrolytic enzym es studied w er e detected in water sam ple of Ga'ar Soda Lake. Metagenome-derived DNA libraries have focused on m any classes of enzym es, am ong these hydrolytic enzym es were prominent. The results of both m ethods indicated that these soda lakes are rich w ith commercially valuable enzym es.

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Characterization of extremely halophilic Archaea isolated from saline environment in different parts of Turkey

Cited by 15 publications

References 27 publications

Characterization of extracellular esterase and lipase activities from five halophilic archaeal strains

Characterization of extracellular esterase and lipase activities from five halophilic archaeal strains

Diversity of hydrolytic enzymes in haloarchaeal strains isolated from salt lake

Screening of Hydrolytic Extremozymes in Haloalkaliphilic Archaea by Culture and Molecular-based Methods

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