Diversity of hydrolytic enzymes in haloarchaeal strains isolated from salt lake

Makhdoumi, Ali; Amoozegar, Mohammad Ali; Khaledi, E. Mahmodi

doi:10.1007/bf03326255

Cited by 40 publications

(20 citation statements)

References 38 publications

(36 reference statements)

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“…The most enzyme producers belonged to Halorubrum. The phylogenetic analysis revealed that Halorubrum and Haloarcula are two dominant halo archaeal genera that showed high rate enzyme production [31]. Eleviet al (2004) studied the production of amylase, protease and lipase in halo archaea.…”

Section: Volume 56mentioning

confidence: 99%

Isolation, Identification and Extracellular Enzymatic Activity of Culturable Extremely Halophilic Archaea and Bacteria of IncheBoroun Wetland

Rasooli

Amoozegar

Sepahy

et al. 2016

ILNS

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Abstract. Extremely halophilic diversity of IncheBroun wetland located in the north of Iran was investigated by using culture-dependent methods. Sampling was carried out in May and September 2014. In each sampling 4 distinct regions of wetland were analyzed by using complex media like MGM, JCM168, MH1 and an alkaliphilic medium containing 23% salts. After incubation at 40˚C, a total of 406 isolates and 2.1 × 10 6 CFU/ml were obtained in culture media. Among them 361 isolates were obtained from MGM and 39 isolates from JCM 168, 3 isolates from MH1 and 3 isolates from the alkaliphilic media. Initial morphological, biochemical and physiological tests were performed. Production of 4 hydrolytic enzymes by 45 selected strains was assayed qualitatively. A total of 38, 19 and 6 strains were able to produce lipase, DNase and amylase activity. Protease activity was not observed among strains. As total 45 strains were selected randomly and phylogenetic analysis of 16S rRNA was performed for them. Among selected strains 40 isolated strians belonged to Haloarchaea and were belonged to the genera: Haloarcula(30%), Halorubrum(27.5%), Haloferax(17.5%), Halobellus (10%), Halogeometricum(5.2%), Halobacterium(2.6%), Halolamina(2.6%), Halorhabdus (2.6%) and Halostagnicola (2.6%). Haloarcula and Halorubrum were the dominant populations. A total of 5 strains belonged to domain of Bacteria and were similar to members of Rhodovibrio (40%), Pseudomonas (40%) and Salicola (20%).

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Section: Volume 56mentioning

confidence: 99%

Isolation, Identification and Extracellular Enzymatic Activity of Culturable Extremely Halophilic Archaea and Bacteria of IncheBoroun Wetland

Rasooli

Amoozegar

Sepahy

et al. 2016

ILNS

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“…Such demand has generated the search for new enzymes able to support the aggressive conditions of some industrial processes. Today, most enzymes do not show the same performance when they are exposed to extreme conditions such as high temperatures, acid or alkaline pH levels, denaturing, etc 3, 4. This situation has led to extremophile micro‐organism research to understand the metabolic adaptation of these micro‐organisms necessary for them to survive extreme conditions; these modifications are based on diverse enzymatic changes 5…”

Section: Introductionmentioning

confidence: 99%

Halophilic hydrolases as a new tool for the biotechnological industries

et al. 2012

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Halophilic micro-organisms are able to survive in high salt concentrations because they have developed diverse biochemical, structural and physiological modifications, allowing the catalytic synthesis of proteins with interesting physicochemical and structural properties. The main characteristic of halophilic enzymes that allows them to be considered as a novel alternative for use in the biotechnological industries is their polyextremophilicity, i.e. they have the capacity to be thermostable, tolerate a wide range of pH, withstand denaturation and tolerate high salt concentrations. However, there have been relatively few studies on halophilic enzymes, with some being based on their isolation and others on their characterisation. These enzymes are scarcely researched because attention has been focused on other extremophile micro-organisms. Only a few industrial applications of halophilic enzymes, principally in the fermented food, textile, pharmaceutical and leather industries, have been reported. However, it is important to investigate applications of these enzymes in more biotechnological processes at both the chemical and the molecular level. This review discusses the modifications of these enzymes, their industrial applications and research perspectives in different biotechnological areas.

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“…The production of other hydrolytic enzymes was studied qualitatively in haloarchaeal strains isolated from the AranBidgol hypersaline lake in the central desert area of Iran [265] and the Tuzkoy salt mine in Central Anatolia, Turkey. Inulinase production by Halorubrum, Haloarcula and Natrinema, DNase production by Halorubrum and Haloarcula, and gelatinase production by Haloarcula and Natrinema have been reported from the Tuzkoy salt mine and Aran-Bidgol hypersaline lake [266].…”

Section: Other Hydrolytic Enzymesmentioning

confidence: 99%

Systematics of haloarchaea and biotechnological potential of their hydrolytic enzymes

et al. 2017

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Halophilic archaea, also referred to as haloarchaea, dominate hypersaline environments. To survive under such extreme conditions, haloarchaea and their enzymes have evolved to function optimally in environments with high salt concentrations and, sometimes, with extreme pH and temperatures. These features make haloarchaea attractive sources of a wide variety of biotechnological products, such as hydrolytic enzymes, with numerous potential applications in biotechnology. The unique trait of haloarchaeal enzymes, haloenzymes, to sustain activity under hypersaline conditions has extended the range of already-available biocatalysts and industrial processes in which high salt concentrations inhibit the activity of regular enzymes. In addition to their halostable properties, haloenzymes can also withstand other conditions such as extreme pH and temperature. In spite of these benefits, the industrial potential of these natural catalysts remains largely unexplored, with only a few characterized extracellular hydrolases. Because of the applied impact of haloarchaea and their specific ability to live in the presence of high salt concentrations, studies on their systematics have intensified in recent years, identifying many new genera and species. This review summarizes the current status of the haloarchaeal genera and species, and discusses the properties of haloenzymes and their potential industrial applications. SYSTEMATICS OF HALOPHILIC ARCHAEAHalophilic micro-organisms are found in the three domains of life, and are extremophiles living in hypersaline environments in the presence of salt (generally NaCl) as a specific requisite for their growth [1]. Hypersaline environments contain higher salt concentrations than seawater (3.5 % total dissolved salts) [2]. According to the optimum NaCl concentration for growth, micro-organisms fall into one of the following categories: extreme halophiles with optimum growth at 15-30 % (2.5-5.2 M) NaCl; moderate halophiles with optimum growth at 3-15 % (0.5-2.5 M) NaCl; slight halophiles with optimum growth at 1-3 % (0.2-0.5 M) NaCl; non-halophiles with optimum growth at less than 1 % (0.2 M) NaCl; and halotolerant micro-organisms which are non-halophiles showing the ability to tolerate high NaCl concentrations [3]. Halophiles can live in hypersaline environments because they are able to sustain osmotic balance. They use two main strategies to withstand the adverse consequences of water loss when exposed to high salt concentrations. The first strategy used by extremely halophilic archaea (haloarchaea) and halophilic anaerobic bacteria such as members of Halanaerobiales is known as the 'salt-in-cytoplasm' strategy, during which salts such as NaCl or KCl accumulate in the cytoplasm at concentrations similar to the extracellular ones [4,5]. Another mechanism to cope with high salt concentrations is the accumulation of organic molecules such as amino acids, sugars and polyols, known as compatible solutes or osmolytes. Compatible solutes are small and highly soluble molecules that can...

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Diversity of hydrolytic enzymes in haloarchaeal strains isolated from salt lake

Cited by 40 publications

References 38 publications

Isolation, Identification and Extracellular Enzymatic Activity of Culturable Extremely Halophilic Archaea and Bacteria of IncheBoroun Wetland

Isolation, Identification and Extracellular Enzymatic Activity of Culturable Extremely Halophilic Archaea and Bacteria of IncheBoroun Wetland

Halophilic hydrolases as a new tool for the biotechnological industries

Systematics of haloarchaea and biotechnological potential of their hydrolytic enzymes

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