Characterization of extracellular polymeric substances produced during nitrate removal by a thermophilic bacterium Chelatococcus daeguensis TAD1 in batch cultures

Wei, Zhendong; Huang, Shaobin; Zhang, Yongqing; Han, Li; Zhou, Shaofeng

doi:10.1039/c7ra08147b

Cited by 20 publications

(7 citation statements)

References 40 publications

(44 reference statements)

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“…Noteworthy, the second most abundant bacilli isolate, A. pallidus (formerly Geobacillus pallidus ), is thermophilic and alkalitolerant ( Minana-Galbis et al, 2010 ) for whom no Xyl activity has been reported in prior studies. That also applies to the Alphaproteobacteria C. daeguensis , a typical mesophilic bacteria ( Yoon et al, 2008 ), for which thermophilic strains have been recently reported ( Wei et al, 2017 ) while having no Xyl activity detected to date. Actinobacteria also harbor Xyl enzymes; although Microbacterium is not the typical genus of that phylum for such activity, some strains having Xyl activity have been isolated from cricket gut ( Kim et al, 2014 ).…”

Section: Resultsmentioning

confidence: 94%

Characterization of Thermophilic Lignocellulolytic Microorganisms in Composting

et al. 2021

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Composting involves the selection of a microbiota capable of resisting the high temperatures generated during the process and degrading the lignocellulose. A deep understanding of the thermophilic microbial community involved in such biotransformation is valuable to improve composting efficiency and to provide thermostable biomass-degrading enzymes for biorefinery. This study investigated the lignocellulose-degrading thermophilic microbial culturome at all the stages of plant waste composting, focusing on the dynamics, enzymes, and thermotolerance of each member of such a community. The results revealed that 58% of holocellulose (cellulose plus hemicellulose) and 7% of lignin were degraded at the end of composting. The whole fungal thermophilic population exhibited lignocellulose-degrading activity, whereas roughly 8–10% of thermophilic bacteria had this trait, although exclusively for hemicellulose degradation (xylan-degrading). Because of the prevalence of both groups, their enzymatic activity, and the wide spectrum of thermotolerance, they play a key role in the breakdown of hemicellulose during the entire process, whereas the degradation of cellulose and lignin is restricted to the activity of a few thermophilic fungi that persists at the end of the process. The xylanolytic bacterial isolates (159 strains) included mostly members of Firmicutes (96%) as well as a few representatives of Actinobacteria (2%) and Proteobacteria (2%). The most prevalent species were Bacillus licheniformis and Aeribacillus pallidus. Thermophilic fungi (27 strains) comprised only four species, namely Thermomyces lanuginosus, Talaromyces thermophilus, Aspergillus fumigatus, and Gibellulopsis nigrescens, of whom A. fumigatus and T. lanuginosus dominated. Several strains of the same species evolved distinctly at the stages of composting showing phenotypes with different thermotolerance and new enzyme expression, even not previously described for the species, as a response to the changing composting environment. Strains of Bacillus thermoamylovorans, Geobacillus thermodenitrificans, T. lanuginosus, and A. fumigatus exhibiting considerable enzyme activities were selected as potential candidates for the production of thermozymes. This study lays a foundation to further investigate the mechanisms of adaptation and acquisition of new traits among thermophilic lignocellulolytic microorganisms during composting as well as their potential utility in biotechnological processing.

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Section: Resultsmentioning

confidence: 94%

Characterization of Thermophilic Lignocellulolytic Microorganisms in Composting

et al. 2021

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“…Since the denitrification experiments were carried out under the same experimental conditions, only by changing the bacteria support, it seems that the presence of carbon in the composites clearly reduces the EPS formation, which could suggest a more “friendly” interaction between the bacteria and support. Nevertheless, thermophilic bacteria simultaneously accumulate EPS during the nitrate reduction [ 36 ], with a positive correlation between the EPS production and the nitrate removal efficiency.…”

Section: Resultsmentioning

confidence: 99%

Supported Biofilms on Carbon–Oxide Composites for Nitrate Reduction in Agricultural Waste Water

et al. 2021

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Escherichia coli colonies were grown on different supports for the removal of nitrates from water. A carbon material and different commercial metal oxides, such as SiO2, TiO2 and Al2O3, and their corresponding carbon–metal oxide composites were studied. The physicochemical properties were analyzed by different techniques and the results were correlated with their performance in the denitrification process. Developed biofilms effectively adhere to the supports and always reach the complete reduction of nitrates to gaseous products. Nevertheless, faster processes occur when the biofilm is supported on mesoporous and non-acid materials (carbon and silica).

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“…It has been suggested that bacterial cell hydrophobicity is influenced by the ability to synthesize exopolysaccharides [37]. It is also claimed that extracellular proteins in EPSs have major relevance in the protective function, as they contribute to the bacterial surface hydrophobicity [38].…”

Section: Comparison Of the Selected Technological And Functional Properties Of The Chosen Autochthonous Labmentioning

confidence: 99%

Spontaneously Fermented Fruiting Bodies of Agaricus bisporus as a Valuable Source of New Isolates of Lactic Acid Bacteria with Functional Potential

Skrzypczak

Gustaw

Jabłońska‐Ryś

et al. 2020

Foods

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The aim of the investigation was the identification and initial study of lactic acid bacteria (LAB) isolated from spontaneously fermented (at 28 °C for 5 days) fruiting bodies of white button mushrooms (Agaricus bisporus). The isolated LAB were preliminarily characterized applying the MALDI-TOF Biotyper. Moreover, further phenotypical, genotypical characteristics as well as some functional and technological properties of the selected microorganisms (including the ability to produce exopolysaccharides, cell hydrophobicity, resistance to low pH, and bile salt) were also analyzed. Among autochthonous LAB (isolated from the tested mushroom raw material), Leuconostoc mesenteroides predominated in spontaneously fermented A. bisporus, while Lactiplantibacillus paraplantarum, Lactiplantibacillus plantarum, and Lactococcus lactis were less abundant. The highest dynamics of acidification of the mushroom material were exhibited by isolates EK55 and EK4 that, after 24 h of incubation, were able to decrease the pH of the raw material up to 5.06 ± 0.021 and 5.17 ± 0.015, respectively. Furthermore, the analysis of bacterial cell hydrophobicity indicated that the highest values of this parameter were noted for L. plantarum isolates EK12 (29.59 ± 0.7%), EK55 (28.75 ± 0.551%), and EK5 (27.33 ± 1.516%). It was revealed some of the analyzed LAB (especially isolates L. plantarum EK55 and L. paraplantarum EK4) exhibited functional and technological potential that might be used in the formulation of novel starter cultures.

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Characterization of extracellular polymeric substances produced during nitrate removal by a thermophilic bacterium Chelatococcus daeguensis TAD1 in batch cultures

Abstract: Positive correlation was observed between EPS production and nitrate removal efficiency during aerobic denitrification byChelatococcus daeguensisTAD1.

Cited by 20 publications

References 40 publications

Characterization of Thermophilic Lignocellulolytic Microorganisms in Composting

Characterization of Thermophilic Lignocellulolytic Microorganisms in Composting

Supported Biofilms on Carbon–Oxide Composites for Nitrate Reduction in Agricultural Waste Water

Spontaneously Fermented Fruiting Bodies of Agaricus bisporus as a Valuable Source of New Isolates of Lactic Acid Bacteria with Functional Potential

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