Characterization of extracellular matrix modified poly(ε-caprolactone) electrospun scaffolds with differing fiber orientations for corneal stroma regeneration

Fernández‐Pérez, Julia; Kador, Karl E.; Lynch, Amy; Ahearne, Mark

doi:10.1016/j.msec.2019.110415

Cited by 67 publications

(54 citation statements)

References 55 publications

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“…Thus, a hydrophilic effect was evident in case of the antibiotic addition, where the 5 wt % of the CF, Figure 6 e, resulted in a water contact angle lower than 90° after 5 s, while the 25 wt % of the CF, Figure 6 f, showed immediate water drop absorption which suggests high wetting effect. Similarly, in a study of PCL electrospun scaffolds modified with ECM for corneal stroma regeneration, the pure PCL scaffolds were reported to have a water contact angle of 121.2° ± 2.0°, while the addition of the ECM had a reduction effect of this value [ 47 ]. Another study discussed the increase in PCL surface wettability with the incorporation of metformin hydrochloride or metoprolol tartrate drugs by blend electrospinning.…”

Section: Resultsmentioning

confidence: 99%

Poly(ε-caprolactone) Titanium Dioxide and Cefuroxime Antimicrobial Scaffolds for Cultivation of Human Limbal Stem Cells

Trcin

Zdraveva

Dolenec³

et al. 2020

Polymers

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Limbal Stem Cell Deficiency (LSCD) is a very serious and painful disease that often results in impaired vision. Cultivation of limbal stem cells for clinical application is usually performed on carriers such as amniotic membrane or surgical fibrin gel. Transplantation of these grafts is associated with the risk of local postoperative infection that can destroy the graft and devoid therapeutic benefit. For this reason, electrospun scaffolds are good alternatives, as proven to mimic the natural cells surroundings, while their fabrication technique is versatile with regard to polymer functionalization and scaffolds architecture. This study considers the development of poly(ε-caprolactone) (PCL) immune-compatible and biodegradable electrospun scaffolds, comprising cefuroxime (CF) or titanium dioxide (TiO2) active components, that provide both bactericidal activity against eye infections and support of limbal stem cells growth in vitro. The PCL/CF scaffolds were prepared by blend electrospinning, while functionalization with the TiO2 particles was performed by ultrasonic post-processing treatment. The fabricated scaffolds were evaluated in regard to their physical structure, wetting ability, static and dynamic mechanical behaviour, antimicrobial efficiency and drug release, through scanning electron microscopy, water contact angle measurement, tensile testing and dynamic mechanical analysis, antimicrobial tests and UV-Vis spectroscopy, respectively. Human limbal stem cells, isolated from surgical remains of human cadaveric cornea, were cultured on the PCL/CF and PCL/TiO2 scaffolds and further identified through immunocytochemistry in terms of cell type thus were stained against p63 marker for limbal stem cells, a nuclear transcription factor and cytokeratin 3 (CK3), a corneal epithelial differentiation marker. The electrospun PCL/CF and PCL/TiO2 successfully supported the adhesion, proliferation and differentiation of the cultivated limbal cells and provided the antimicrobial effect against Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans.

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Section: Resultsmentioning

confidence: 99%

Poly(ε-caprolactone) Titanium Dioxide and Cefuroxime Antimicrobial Scaffolds for Cultivation of Human Limbal Stem Cells

Trcin

Zdraveva

Dolenec³

et al. 2020

Polymers

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“…[22b,51] Radially aligned fibers can be produced using a cup and pin collector where the fibers align between the pin and outer circular ring (or cup). [247] To obtain orthogonally orientated fibers a negatively charged collector connected to four separate posts can be used. [247] The negative charge switches between the two horizontal posts and the two vertical posts at a specified rate.…”

Section: Electrospinningmentioning

confidence: 99%

“…[247] To obtain orthogonally orientated fibers a negatively charged collector connected to four separate posts can be used. [247] The negative charge switches between the two horizontal posts and the two vertical posts at a specified rate. When the horizontal posts are charged the fibers are attached toward these and aligned horizontally between them.…”

Section: Electrospinningmentioning

confidence: 99%

“…The fibers can be removed from the collector using cellulose frames that adhere to be fibers, allowing their orientation to remain fixed 22b,51. Radially aligned fibers can be produced using a cup and pin collector where the fibers align between the pin and outer circular ring (or cup) 247. To obtain orthogonally orientated fibers a negatively charged collector connected to four separate posts can be used 247.…”

Section: Fabrication Processesmentioning

confidence: 99%

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Designing Scaffolds for Corneal Regeneration

Ahearne

Fernández‐Pérez

Masterton

et al. 2020

Adv Funct Materials

Self Cite

120

109

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Corneal blindness is one of the most common causes of vision loss worldwide, affecting millions of people. To treat these patients, researchers have been examining different approaches to engineer corneal scaffolds suitable for transplantation. Scaffolds have been developed to replace part or all of the cornea depending on the patient requirements. Both acellular and cell-seeded scaffolds have been tested in animal models. Materials that have been under investigation for manufacturing scaffolds include collagen, silk fibroin, amniotic membrane, decellularized cornea, fibrin, chitosan, gelatin, agarose, alginate, and hyaluronic acid in addition to several synthetic polymers. Different combinations of materials, fiber crosslinking techniques, and incorporation of bioactive molecules have also been examined. Factors such as the physical properties, cytocompatibility, degradation behavior, and optical characteristics have to be considered when selecting a suitable scaffold material. Recent advancements in materials fabrication techniques such as bioprinting, electrospinning, and different collagen alignment techniques, allow scaffolds to be generated that more accurately mimic the structure of the corneal stroma. A number of scaffolds have commenced clinical trials to determine their suitability for corneal regeneration.

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“…Moreover, for many applications, in which is necessary to control the orientation of nanofibers and their alignment, a rotating collector can be used. Several research works have been carried out involving the use of a rotating drum ( Figure 3 b) in different fields such as developing new material for high-technologies [ 44 ], piezoelectric materials [ 45 ] or medical applications [ 46 ]. Moreover, template electrospinning consists in a collector with geometrical pattern configuration which deeply influences the deposition of electrospun nanofibers ( Figure 3 c).…”

Section: Electrospinning Technique and Post-processing Methodsmentioning

confidence: 99%

Potential Applications of Magnesium-Based Polymeric Nanocomposites Obtained by Electrospinning Technique

et al. 2020

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In the last few decades, the development of new electrospun materials with different morphologies and advanced multifunctional properties are strongly consolidated. There are several reviews that describe the processing, use and characterization of electrospun nanocomposites, however, based on our knowledge, no review on electrospun nanocomposites reinforced with nanoparticles (NPs) based on magnesium, Mg-based NPs, are reported. Therefore, in the present review, we focus attention on the fabrication of these promising electrospun materials and their potential applications. Firstly, the electrospinning technique and its main processing window-parameters are described, as well as some post-processing methods used to obtain Mg-based materials. Then, the applications of Mg-based electrospun nanocomposites in different fields are pointed out, thus taking into account the current trend in developing inorganic-organic nanocomposites to gradually satisfy the challenges that the industry generates. Mg-based electrospun nanocomposites are becoming an attractive field of research for environmental remediation (waste-water cleaning and air filtration) as well as for novel technical textiles. However, the mayor application of Mg-based electrospun materials is in the biomedical field, as pointed out. Therefore, this review aims to clarify the tendency in using electrospinning technique and Mg-based nanoparticles to huge development at industrial level in the near future.

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Characterization of extracellular matrix modified poly(ε-caprolactone) electrospun scaffolds with differing fiber orientations for corneal stroma regeneration

Cited by 67 publications

References 55 publications

Poly(ε-caprolactone) Titanium Dioxide and Cefuroxime Antimicrobial Scaffolds for Cultivation of Human Limbal Stem Cells

Poly(ε-caprolactone) Titanium Dioxide and Cefuroxime Antimicrobial Scaffolds for Cultivation of Human Limbal Stem Cells

Designing Scaffolds for Corneal Regeneration

Potential Applications of Magnesium-Based Polymeric Nanocomposites Obtained by Electrospinning Technique

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