Characterization of extracellular and cell bound biosurfactants produced by Aneurinibacillus aneurinilyticus isolated from commercial corn steep liquor

López-Prieto, Alejandro; Rodríguez‐López, Lorena; Rincón-Fontán, M.; Cruz, J.M.

doi:10.1016/j.micres.2020.126614

Cited by 27 publications

(25 citation statements)

References 28 publications

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“…The endospore-forming Bacillus strain, isolated from CSW in previous works, has been identified as Aneurinibacillus aneurinilyticus and corresponds to the strain deposited in the Spanish Type Culture Collection (CECT) (Valencia, Spain), which was identified as CECT 9939. , This microorganism was grown at 37 °C for 48 h in 200 mL of tryptic soy broth (TSB), previously sterilized at 121 °C for 15 min. The biomass was separated from the culture media by centrifugation at 5000 rpm in a ROTINA 380R (Hettich, Germany) in order to extract two different biosurfactants, one from the fermented broth (BS3) and other from Bacillus cells (BS4).…”

Section: Methodsmentioning

confidence: 99%

Characterization and Cytotoxic Effect of Biosurfactants Obtained from Different Sources

et al. 2020

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In this work, five biosurfactant extracts, obtained from different sources, all of them with demonstrated antimicrobial properties, were characterized and subjected to a cytotoxic study using mouse fibroblast cells (NCTC clone 929). Biosurfactant extracts obtained directly from corn steep water (CSW) showed similar surfactant characteristics to those of the extracellular biosurfactant extract produced by Bacillus isolated from CSW and grown in tryptic soy broth, observing that they are amphoteric, consisting of viscous and yellowish liquid with no foaming capacity. Contrarily, cell-bound biosurfactant extracts produced from Lactobacillus pentosus or produced by Bacillus sp isolated from CSW are nonionic, consisting of a white powder with foaming capacity. All the biosurfactants possess a similar fatty acid composition. The cytotoxic test revealed that the extracts under evaluation, at a concentration of 1 g/L, were not cytotoxic for fibroblasts (fibroblast growth > 90%). The biosurfactant extract obtained from CSW with ethyl acetate, at 1 g/ L, showed the highest cytotoxic effect but above the cytotoxicity limit established by the UNE-EN-ISO10993-5. It is remarkable that the cell-bound biosurfactant produced by L. pentosus, at a concentration of 1 g/L, promoted the growth of the fibroblast up to 113%.

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Section: Methodsmentioning

confidence: 99%

Characterization and Cytotoxic Effect of Biosurfactants Obtained from Different Sources

et al. 2020

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“…A recent publication showed that a Bacillus strain, Aneurinibacillus aneurinilyticus , is responsible for the spontaneous production of BS in CSL 22 . Likewise, Rodríguez-López et al 23 have characterized and identified a BS extracted with chloroform from CSL as a lipopeptide composed mainly by C16-C18 fatty acids and amino acids.…”

Section: Introductionmentioning

confidence: 99%

Solubilization of cuprous oxide in water using biosurfactant extracts from corn steep liquor: a comparative study

López-Prieto

Moldes

Cruz

et al. 2022

Sci Rep

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In this work the chemical characterization (elemental analysis and the content of phospholipids) and surface-active properties of two biosurfactants (BS) extracted with both chloroform or ethyl acetate from corn steep liquor were compared. The phospholipids content for the BS extracted with chloroform (BS1) was considerably higher (8.15%) than that obtained for the BS extracted with ethyl acetate (BS2), 0.11%. When comparing the FTIR spectra of the two BS studied in this work with the spectrum of the commercial surfactant lecithin, a greater similarity (75%) was observed with the spectrum of the BS1. The biosurfactant extract (BS2) provided the most favorable conditions for the solubilization of cuprous oxide (Cu-Ox) in water (12.54% of copper dissolved), in comparison with BS1. The results achieved were considerably better than those obtained with chemical surfactants (Tween 80, SDS and CTAB) on solubilizing Cu-Ox, resulting in the latter cases on percentages of Cu dissolved always lower than 0.21%. In addition, a factorial design was performed obtaining the optimum conditions to solubilize Cu-Ox, where the maximum water solubility of Cu-Ox (13.17%) was achieved using 3.93 g/L of BS2 with a contact time of 19.86 min and using a concentration of Cu-Ox of 1.96 g/L. Thus, the BS2 extract could have a promising future as solubilizing agent in the formulation of more sustainable Cu-Ox based pesticides. Moreover, it was confirmed that the presence of phospholipids prevents the solubilization of copper-based pesticides in water.

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“…aneurinilyticus has been reported to exist in fermented products. 21,22 In stinky tofu brine, it was one of the main associated bacteria that were significantly positively correlated with key flavor substances and involved in the metabolism of aromatic amino acids. 22 Several A. aneurinilyticus, including strain A. aneurinilyticus BKT-9 and A. aneurinilyticus strain DBT87, were reported to have the ability of cellulose degrading.…”

Section: Isolation and Identification Of Cyfluthrin-degrading Speciesmentioning

confidence: 99%

Microbial Fermentation as an Efficient Method for Eliminating Pyrethroid Pesticide Residues in Food: A Case Study on Cyfluthrin and Aneurinibacillus aneurinilyticus D-21

Yu,

Wang,

Tang

et al. 2024

J. Agric. Food Chem.

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The microbial fermentation of food has emerged as an efficient means to eliminate pesticide residues in agricultural products; however, the specific degradation characteristics and mechanisms remain unclear. In this study, a Gram-positive bacterium, Aneurinibacillus aneurinilyticus D-21, isolated from fermented Pixian Douban samples exhibited the capability to degrade 45 mg/L of cyfluthrin with an efficiency of 90.37%. Product analysis unveiled a novel cyfluthrin degradation pathway, involving the removal of the cyanide group and ammoniation of the ester bond into an amide. Whole genome analysis discovered the enzymes linked to cyfluthrin degradation, including nitrilase, esterase, carbon−nitrogen ligases, and enzymes associated with aromatic degradation. Additionally, metabolome analysis identified 140 benzenoids distributed across various aromatic metabolic pathways, further substantiating D-21's catabolic capability toward aromatics. This study underscores the exceptional pyrethroid degradation prowess of A. aneurinilyticus D-21, positioning it as a promising candidate for the biotreatment of pesticide residues in food systems.

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Characterization of extracellular and cell bound biosurfactants produced by Aneurinibacillus aneurinilyticus isolated from commercial corn steep liquor

Cited by 27 publications

References 28 publications

Characterization and Cytotoxic Effect of Biosurfactants Obtained from Different Sources

Characterization and Cytotoxic Effect of Biosurfactants Obtained from Different Sources

Solubilization of cuprous oxide in water using biosurfactant extracts from corn steep liquor: a comparative study

Microbial Fermentation as an Efficient Method for Eliminating Pyrethroid Pesticide Residues in Food: A Case Study on Cyfluthrin and Aneurinibacillus aneurinilyticus D-21

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