Characterization of estrogen receptors alpha and beta in uterine leiomyoma cells

Valladares, Francisco; Frı́as, I.; Báez, Delia; García, Cristina Moya; López, Francisco J.; Fraser, James D.; Rodríguez, Yasmina; Reyes, Ricardo; Díaz‐Flores, Lucio; Bello, Aixa R.

doi:10.1016/j.fertnstert.2006.05.047

Cited by 21 publications

(17 citation statements)

References 32 publications

(39 reference statements)

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“…Therefore, the gene expression data in this present investigation of 14 postmenopausal tissues proved that up-regulation of ER␤ initially occurred at the level of transcription in myoma cells, whereas mRNA levels of ER␣ and PGR in myomas remained similar to patient matched myometrium. Differences of higher ER␣ protein levels observed in postmenopausal myomas compared to myometrim [20,21] could be explained by post-transcriptional regulation.…”

Section: Discussionmentioning

confidence: 95%

“…Kovacs et al [20] showed in a small patient cohort of 4 postmenopausal myomas, that ER␤ protein levels increased compared to myometrium however, ER␣ protein levels determined in 16 premenopausal myomas remained high, but did not increase further postmenopausally. Using immunohistochemistry, Valladares et al [21], found a rise of both ER␣ and ER␤ in a small cohort of postmenopausal myomas compared to premenopausal myomas. Therefore, the gene expression data in this present investigation of 14 postmenopausal tissues proved that up-regulation of ER␤ initially occurred at the level of transcription in myoma cells, whereas mRNA levels of ER␣ and PGR in myomas remained similar to patient matched myometrium.…”

Section: Discussionmentioning

confidence: 99%

“…Examining the mRNA and protein levels of ER␣ and ER␤ in premenopausal myoma tissues compared to matched normal myometrium have also demonstrated that ER␣ presided over ER␤ [18]. There are only a few studies in the literature regarding hormone regulation and steroid hormone receptor status in postmenopausal normal myometrium [19] and myomas [17,20,21]. Therefore, to gain more insight into the tumor biology of myomas the ER␣, ER␤ and PGR mRNA levels of myomas and patient-matched myometrium were determined in a cohort of postmenopausal women (n = 14) using semi-quantitative Realtime PCR and RT-PCR.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptional analysis of steroid hormone receptors in smooth muscle uterine leiomyoma tumors of postmenopausal patients

Strissel

Swiatek

Oppelt

et al. 2007

The Journal of Steroid Biochemistry and Molecular Biology

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transcriptional analysis of steroid hormone receptors in smooth muscle uterine leiomyoma tumors of postmenopausal patients

Strissel

Swiatek

Oppelt

et al. 2007

The Journal of Steroid Biochemistry and Molecular Biology

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“…Thus, agents that can decrease the inherently high ERa-to-ERb ratio of MCF-7 cells may also inhibit the estrogen-dependent proliferation of these cells, apparently by improving promoter occupancy of ER target genes by ERb relative to ERa. Whether such agents could have an impact on the growth of fibroid smooth muscle cells, which are known to express ERb as well as ERa (18), is presently a matter of conjecture.…”

Section: Figurementioning

confidence: 99%

“…Although endothelial and connective tissue cells of leiomyomas and normal myometrium express only ERb, smooth muscle cells express both ER subtypes (18). Whether and how the ERa-to-ERb expression ratio could influence the estrogen-dependent development of leiomyomas is not known presently.…”

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confidence: 99%

Estrogen receptor α and β in uterine fibroids: a basis for altered estrogen responsiveness

Bakas

Liapis

Vlahopoulos

et al. 2008

Fertility and Sterility

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Objective: To investigate the relative expression and the DNA-binding status of estrogen receptors a and b in fibroids and normal myometrial tissue to explore the molecular basis of altered estrogen responsiveness of leiomyomas. Design: Biopsy samples from uterine fibroids and adjacent normal myometrial tissue at the follicular phase of the menstrual cycle. Result(s): The level of messenger RNA expression of estrogen receptor a and b and the level of estrogen receptor as a whole are increased on average to a similar extent in leiomyomas compared with normal myometrium. Occasionally, however, estrogen receptor a is disproportionately increased in leiomyomas, and this appears to increase the amount of estrogen receptor a that binds to the estrogen-responsive element of estrogen target genes as homodimer rather than as heterodimer with estrogen receptor b. Conclusion(s):The estrogen receptor a-to-estrogen receptor b expression ratio rather than the individual expression levels determines the fraction of DNA-binding homodimers of estrogen receptor a and possibly the growth potential of myomas. (Fertil Steril Ò 2008;-:---.

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Distribution patterns of estrogen receptor α and β in the human cortex and hippocampus during development and adulthood

González

Cabrera-Socorro

Pérez-García

et al. 2007

J of Comparative Neurology

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The expression of estrogen receptors (ERs) in the developing and adult human brain has not been clearly established, although estrogens are crucial for neuronal differentiation, synapse formation, and cognitive functions. By using immunohistochemistry, we have studied the distribution of ER alpha and ER beta in human cerebral cortex and hippocampus from early prenatal stages to adult life. ER alpha was detected in the cortex at 9 gestational weeks (GW), with a high expression in proliferating zones and the cortical plate. The staining intensity decreased gradually during prenatal development but increased again from birth to adulthood. In contrast, ER beta was first detected at 15 GW in proliferating zones, and at 16/17 GW, numerous ER beta immunopositive cells were also observed in the cortical plate. ER beta expression persisted in the adult cortex, being widely distributed throughout cortical layers II-VI. In addition, from around 15 GW to adulthood, ER alpha and ER beta were expressed in human hippocampus mainly in pyramidal cells of Ammon's horn and in the dentate gyrus. Western blotting and immunohistochemistry in the adult cerebral cortex and hippocampus revealed lower protein expression of ER alpha compared with ER beta. Double immunostaining showed that during fetal life both ERs are expressed in neurons as well as in radial glia, although only ER alpha is expressed in the Cajal-Retzius neurons of the marginal zone. These observations demonstrate that the expression of ER alpha and ER beta displays different spatial-temporal patterns during human cortical and hippocampal development and suggest that both ERs may play distinct roles in several processes related to prenatal brain development.

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Characterization of estrogen receptors alpha and beta in uterine leiomyoma cells

Cited by 21 publications

References 32 publications

Transcriptional analysis of steroid hormone receptors in smooth muscle uterine leiomyoma tumors of postmenopausal patients

Transcriptional analysis of steroid hormone receptors in smooth muscle uterine leiomyoma tumors of postmenopausal patients

Estrogen receptor α and β in uterine fibroids: a basis for altered estrogen responsiveness

Distribution patterns of estrogen receptor α and β in the human cortex and hippocampus during development and adulthood

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