Characterization of Erwinia chrysanthemi biovars in alpine water sources by biochemical properties, GLC fatty acid analysis and genomic DNA fingerprinting

Cother, E. J.; Bradley, J.; Gillings, Michael R.; Fahy, P. C.

doi:10.1111/j.1365-2672.1992.tb01694.x

Cited by 28 publications

(22 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These strains and also the D. dianthicola strains found in rivers in southern Finland may originate from imported ornamentals, fruit and other products. Alternatively, the atypical, weakly pathogenic strains may be natural free-living inhabitants of water, as suggested earlier by Cother et al (1992).…”

Section: Discussionmentioning

confidence: 93%

Symptoms and yield reduction caused by Dickeya spp. strains isolated from potato and river water in Finland

et al. 2009

View full text Add to dashboard Cite

Biochemical characterisation of Dickeya strains isolated from potato plants and river water samples in Finland showed that the majority of the strains were biovar 3. They thus resembled the strains recently isolated from potato in the Netherlands, Poland and Israel and form a new clade within the Dickeya genus. About half of the Finnish isolates resembling strains within this new clade were virulent and caused wilting, necrotic lesions and rotting of leaves and stems. Similar symptoms were caused by D. dianthicola strains isolated from one potato sample and from several river water samples. Frequently, the rotting caused by the Dickeya strains was visible in the upper parts of the stem, while the stem base was necrotic from the pith but hard and green on the outside, resulting in symptoms quite different from the blackleg caused by Pectobacterium atrosepticum. The presence of Dickeya in the symptomatic plants in the field assay was verified with a conventional PCR and with a real-time PCR test developed for the purpose. The virulent Dickeya strains reduced the yield of individual plants by up to 50% and caused rotting of the daughter tubers in the field and in storage. Management of Dickeya spp. in the potato production chain requires awareness of the symptoms and extensive knowledge about the epidemiology of the disease.

show abstract

Section: Discussionmentioning

confidence: 93%

Symptoms and yield reduction caused by Dickeya spp. strains isolated from potato and river water in Finland

et al. 2009

View full text Add to dashboard Cite

show abstract

“…For biovar 1 and 7 strains, however, no assimilation of inulin was observed. Cother et al (1992) also described D. dianthicola strains negative for inulin. Furthermore, Samson et al (2005) described 12% of the phenon 5 D. dianthicola Fig.…”

Section: Discussionmentioning

confidence: 99%

“…The strains may have been introduced in seed potatoes by the use of contaminated surface water for irrigation. Surface water contaminated with Dickeya and Pectobacterium has been frequently found (Pérombelon andHyman 1987, Cappaert et al 1988;Persson 1991;Cother et al 1992;Norman et al 2003). It has been speculated that contaminated surface water can be a primary source of infection for clean potato stocks (Cappaert et al 1988;Laurila et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Biochemical and genetical analysis reveal a new clade of biovar 3 Dickeya spp. strains isolated from potato in Europe

Slawiak

Beckhoven²,

Speksnijder³

et al. 2009

Eur J Plant Pathol

185

View full text Add to dashboard Cite

Sixty-five potato strains of the soft rotcausing plant pathogenic bacterium Dickeya spp., and two strains from hyacinth, were characterised using biochemical assays, REP-PCR genomic finger printing, 16S rDNA and dnaX sequence analysis. These methods were compared with nineteen strains representing six Dickeya species which included the type strains. A group of twenty-two potato strains isolated between 2005-2007 in the Netherlands, Poland, Finland and Israel were characterised as belonging to biovar 3. They were 100% identical in REP-PCR, dnaX and 16S rDNA sequence analysis. In a polyphasic analysis they formed a new clade different from the six Dickeya species previously described, and may therefore constitute a new species. The strains were very similar to a Dutch strain from hyacinth. On the basis of dnaX sequences and biochemical assays, all other potato strains isolated in Europe between 1979 and 1994 were identified as D. dianthicola (biovar 1 and 7), with the exception of two German strains classified as D. dieffenbachia (biovar 2) and D. dadantii (biovar 3), respectively. Potato strains from Peru were classified as D. dadantii, from Australia as D. zeae and from Taiwan as D. chrysanthemi bv. parthenii, indicating that different Dickeya species are found in association with potato.

show abstract

“…The strains were identified by standard bacteriological methods; (Cother and Powell 1983;Dickey 1979) using the following tests: indole production from tryptophan, production of phosphatase, and sensitivity to erythromycin (Cother et al 1992;Janse and Spit 1989;Pérombelon and van der Wolf 1998). The strains were also classified using the microtiter plate assay described by Palacio-Bielsa et al (2006) with few modifications as described below.…”

Section: Biochemical Identification and Classification Of Strainsmentioning

confidence: 99%

Assessment of recent outbreaks of Dickeya sp. (syn. Erwinia chrysanthemi) slow wilt in potato crops in Israel

et al. 2008

View full text Add to dashboard Cite

Suspected Dickeya sp. strains were obtained from potato plants and tubers collected from commercial plots. The disease was observed on crops of various cultivars grown from seed tubers imported from the Netherlands during the spring seasons of 2004-2006, with disease incidence of 2-30% (10% in average). In addition to typical wilting symptoms on the foliage, in cases of severe infection, progeny tubers were rotten in the soil. Six strains were characterised by biochemical, serological and PCR-amplification. All tests verified the strains as Dickeya sp. The rep-PCR and the biochemical assays showed that the strains isolated from blackleg diseased plants in Israel were very similar, if not identical to strains isolated from Dutch seed potatoes, suggesting that the infection in Israel originated from the Dutch seed. The strains were distantly related to D. dianthicola strains, typically found in potatoes in Western Europe, and were similar to biovar 3 D. dadanti or D. zeae. This is the first time that the presence of biovar 3 strains in potato in the Netherlands is described. One of the strains was used for pathogenicity assays on potato cvs Nicola and Mondial. Symptoms appeared 2 to 3 days after stem inoculation, and 7 to 10 days after soil inoculation. The control plants treated with water, or plants inoculated with Pectobacterium carotovorum, did not develop any symptoms with either method of inoculation. The identity of Dickeya sp. and P. carotovorum re-isolated from inoculated plants was confirmed by PCR and ELISA.

show abstract

Characterization of Erwinia chrysanthemi biovars in alpine water sources by biochemical properties, GLC fatty acid analysis and genomic DNA fingerprinting

Cited by 28 publications

References 17 publications

Symptoms and yield reduction caused by Dickeya spp. strains isolated from potato and river water in Finland

Symptoms and yield reduction caused by Dickeya spp. strains isolated from potato and river water in Finland

Biochemical and genetical analysis reveal a new clade of biovar 3 Dickeya spp. strains isolated from potato in Europe

Assessment of recent outbreaks of Dickeya sp. (syn. Erwinia chrysanthemi) slow wilt in potato crops in Israel

Contact Info

Product

Resources

About