Characterization of equine plasma lipoproteins after separation by density gradient

The isolation, characterisation and quantification of the equine plasma lipoproteins

“…The lipid and protein compositions of the fractions isolated by the three techniques were consistent and agree with the values previously reported for flotation ultracentrifugation (Bauer 1983) and density gradient ultracentrifugation (Le Goff et al 1987). The VLDL isolated by gel filtration had a lower protein content than that isolated by rate-zonal and flotation ultracentrifugation; this could represent non-specific dissociation of apolipoproteins as the particles pass through the gel matrix or superior resolution of this lipoprotein class from other plasma proteins.…”

“…Heterogeneity of equine LDL has been demonstrated by analytical (Leat et al 1979) and density gradient ultracentrifugation (Terpstra et a1 1982;Hollanders et al 1986), and two subfractions with particle sizes and compositions similar to those of LDL2 and LDL3 have been described (Le Goff et al 1987). The superior resolution provided by rate-zonal ultracentrifugation has allowed us to demonstrate a third subfraction (LDLI) early in the LDL density range.…”

“…Fractions with the density characteristics of VLDL, LDL and HDL were found in three horses using gradient ultracentrifugation systems with lipid prestaining (Terpstra, Sanchez-Muniz, West and Woodward 1982;Hollanders et a1 1986). Plasma lipoproteins were recently isolated from six Thoroughbred horses using a similar technique and characterised by their chemical composition and particle size distribution on electron microscopy (Le Goff, Nouvelot, Fresnel and Silberzahn 1987;Le Goff et a1 1989).…”

Characterization of lipoproteins in cerebrospinal fluid of mares during pregnancy and lactation

“…[4][5][6][7][8][9] In other words, for these mammals the LDL with hydrated densities between 1.020 and 1.063 g/ml are electrophoretically heterogeneous. This also is true for horses, [10][11][12][13] although the percentage of α-LDL in equine plasma is much less than that found in plasma of marine mammals and cattle. [4][5][6] Most plasma lipoproteins of horses can be isolated within the HDL class.…”

Avaliação da aceitabilidade e digestibilidade de dietas para eqüinos com diferentes fontes de óleo vegetal

“…Por outro lado, Jansen et al (2000a; ROBIE et al, 1975;LE GOFF et al, 1987VAN DUK;WENSING, 1989;WATSON et al, 1991WATSON et al, , 1993. Esse perfil é bastante similar ao humano, o que valida a utilização dos mesmos métodos laboratoriais para sua quantificação neste trabalho A ingestão por longo tempo de gorduras adicionadas à ração não parece ter efeitos negativos em eqüinos (PAGAN et al, 1995), ao contrário do que ocorre com humanos (ÁGUILA et al, 2005 Silva et al (2005), a substituição, na dieta de ratos, da gordura hidrogenada pelo óleo de palma diminuiu, no plasma, a concentração do colesterol.…”