Characterization of ependymal cells in hypothalamic and choroidal primary cultures

Gabrion, Jacqueline; Peraldi, S; Faivre-Bauman, A.; Klotz, Catherine; Ghandour, Mohamedanwar; Paulin, Denise; Assenmacher, I; Tixier-Vidal, A

doi:10.1016/0306-4522(88)90082-6

Cited by 22 publications

(23 citation statements)

References 31 publications

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“…Choroid plexus cells are truly epithelial in nature as demonstrated by ultrastructural and immunohistochemical studies [6][7][8][9][10][11][12][13][14][15] . Most studies report reactivity to cytokeratins (CK), especially of low molecular weight, vimentin, epithelial membrane antigen (EMA), transthyretin (TTR), S100 protein, glial fibrillary acidic protein (GFAP) and neuron specific enolase (NSE), but the frequency of reaction varies greatly 6,[9][10][11]13,14,[16][17][18][19][20][21] .…”

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“…Most studies report reactivity to cytokeratins (CK), especially of low molecular weight, vimentin, epithelial membrane antigen (EMA), transthyretin (TTR), S100 protein, glial fibrillary acidic protein (GFAP) and neuron specific enolase (NSE), but the frequency of reaction varies greatly 6,[9][10][11]13,14,[16][17][18][19][20][21] . In contrast, normal choroid plexus, fetal or adult, has been described as constantly negative to GFAP, with rare exceptions [7][8][9][10][11][12][13][14][15] . Poorly differentiated choroid plexus CA, especially in pediatric patients with posterior fossa neoplasms, must be distinguished from other anaplastic tumors with solid diffuse pattern and undifferentiated cells, such as the rare and controversial atypical teratoid / rhabdoid tumor (AT/RT) [22][23][24][25] .…”

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confidence: 99%

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Papillomas and carcinomas of the choroid plexus: histological and immunohistochemical studies and comparison with normal fetal choroid plexus

Barreto

Vassallo

Queiróz

2004

Arq. Neuro-Psiquiatr.

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-Background: Choroid plexus tumors are rare. Results on immunohistochemical features are scanty and controversial even regarding normal plexus. Method: Thirteen cases of choroid plexus tumors and five samples of normal fetal choroid plexus were submitted to immunohistochemical study using a panel of epithelial, neuronal and stromal markers. Results/Conclusions: Relevant histological findings were presence of clear cells in 3/5 papillomas (PP) and 7/8 carcinomas (CA) and all 5 fetal plexuses; rhabdoid cells, desmoplasia and vascular proliferation were found respectively in 3, 4 and 5 cases out of 6 poorly differentiated CA and were absent in PP and well differentiated CA. Pancytokeratin AE1/AE3 was strongly positive in all 13 cases, even in the undifferentiated component of poorly differentiated CA, where reactivity was focal in 3 and diffuse in 3 cases. Low molecular weight cytokeratin (35βH11) was not expressed in any of the 8 CA, but was present in all 5 PP. In 4 of 6 poorly differentiated CA there was reactivity for smooth muscle actin (1A4) in 10 to 30% of the cells. This was true also for one case lacking rhabdoid cells. Laminin was undetectable in all 6 cases of poorly differentiated CA but was present in 4 PP and 2 well differentiated CA. All 5 fetal plexuses expressed GFAP.KEY WORDS: choroid plexus tumors, normal fetal choroid plexus, immunohistochemistry, central nervous system. Papilomas e carcinomas do plexo coróide: estudo histológico e imuno-histoquímico e comparação com plexo coróide fetal normal RESUMO -Contexto: Os tumores do plexo coróide são raros. Os resultados de dados imuno-histoquímicos são escassos e controversos, o mesmo valendo para o plexo coróide normal. Método: Treze casos de tumores do plexo coróide e cinco exemplares de plexo coróide fetal normal foram submetidos a estudo imuno-histoquímico, utilizando-se marcadores para antígenos epiteliais, neurais e estromais. Resultados/Conclusão: Os achados histológicos mais relevantes foram células claras em 3/5 papilomas (PP) e 7/8 carcinomas (CA) e em todos os 5 plexos fetais; células rabdóides, desmoplasia e proliferação vascular foram encontradas, respectivamente, em 3, 4 e 5 casos de 6 CA pouco diferenciados, mas não nos PP e CA bem diferenciados. A pancitoqueratina AE1/AE3 foi fortemente positiva em todos os 13 casos, mesmo no componente indiferenciado do CA pouco diferenciado, em que a reatividade foi focal em 3 casos e difusa em outros 3. A citoqueratina de baixo peso molecular (35βH11) não foi expressa em nenhum dos 8 CA, mas estava presente em todos os 5 PP. Em 4/6 CA pouco diferenciados houve reatividade para actina de músculo liso (1A4) em 10-30% das células. Este achado ocorreu também em um caso sem células rabdóides. Laminina não foi detectada em nenhum dos 6 CA pouco diferenciados, mas estava presente em 4 PP e em 2 CA bem diferenciados. Todos os 5 plexos fetais expressaram GFAP. PALAVRAS-CHAVE: tumores do plexo coróide, plexo coróide fetal normal, imuno-histoquímica, sistema nervoso central.

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confidence: 99%

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confidence: 99%

Papillomas and carcinomas of the choroid plexus: histological and immunohistochemical studies and comparison with normal fetal choroid plexus

Barreto

Vassallo

Queiróz

2004

Arq. Neuro-Psiquiatr.

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“…The establishment of choroidal epithelial cell culture has been described in the literature for the cells from various sources, including mouse (2,6,19), rat (2,6,19,24), rabbit (15), sheep (10), cow (3,25), and human (8). Some of these reports have used transferrin (24), Na + , K + -ATPase (19,25), G proteins (19), ciliary rootlet proteins (6), and the absence of antihemophilic factor (AHF) antigen (3) as the cellular markers to characterize the origin of the cultures.…”

Section: Discussionmentioning

confidence: 99%

“…Some of these reports have used transferrin (24), Na + , K + -ATPase (19,25), G proteins (19), ciliary rootlet proteins (6), and the absence of antihemophilic factor (AHF) antigen (3) as the cellular markers to characterize the origin of the cultures. In the current studies, we chose TTR because choroid plexus epithelium is the only cell type capable of manufacturing TTR in the mammalian brain.…”

Section: Discussionmentioning

confidence: 99%

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Primary culture of choroidal epithelial cells: Characterization of an in vitro model of blood-CSF barrier

Zheng

Zhao

Graziano

1998

In Vitro Cell.Dev.Biol.-Animal

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SummaryA primary rat choroidal epithelial cell culture system was developed to investigate mechanisms of heavy metal toxicity on the blood-cerebrospinal fluid (CSF) barrier. Epithelial cells were dissociated from choroidal tissue by pronase digestion and cultured in standard DMEM culture media supplemented with 10% fetal bovine serum and 10 ng epithelial growth factor per ml. The procedure yielded 2-5 × 10 4 cells from pooled plexuses of three to four rats, and a viability of 77-85%. The cultures displayed a dominant polygonal type of epithelial cells, with a population doubling time of 2-3 d. The cultures were of distinct choroidal epithelial origins. For example, immunocytochemical studies using monospecific rabbit anti-rat TTR polyclonal antibody revealed a strong positive stain of transthyretin (TTR), a thyroxine transport protein exclusively produced by the choroidal epithelia. Also, reverse-transcriptase polymerase chain reaction (PCR) confirmed the presence of specific TTR mRNA in the cultures. The cultures were further adapted to grow on a freely permeable membrane sandwiched between two culture chambers. The formation of an impermeable confluent monolayer occurred within 5 d after seeding and was verified by the presence of a steady electrical resistance across the membrane (80 ± 10 ohm per cm 2 ). The epithelial barriers appeared to actively transport [ 125 I]-thyroxine from the basal to apical chamber. These results suggest that this primary cell culture system possesses typical choroidal epithelial characteristics and appears to be a suitable model for in vitro mechanistic investigations of blood-CSF barrier.

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Ependymal and choroidal cells in culture: Characterization and functional differentiation

Gabrion

Herbuté

Bouillé

et al. 1998

Microsc. Res. Tech.

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Characterization of ependymal cells in hypothalamic and choroidal primary cultures

Cited by 22 publications

References 31 publications

Papillomas and carcinomas of the choroid plexus: histological and immunohistochemical studies and comparison with normal fetal choroid plexus

Papillomas and carcinomas of the choroid plexus: histological and immunohistochemical studies and comparison with normal fetal choroid plexus

Primary culture of choroidal epithelial cells: Characterization of an in vitro model of blood-CSF barrier

Ependymal and choroidal cells in culture: Characterization and functional differentiation

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