Characterization of Enterocoliticin, a Phage Tail-Like Bacteriocin, and Its Effect on Pathogenic
            <i>Yersinia enterocolitica</i>
            Strains

Strauch, Eckhard; Kaspar, Heike; Schaudinn, Christoph; Dersch, Petra; Madela, Kazimierz; Gewinner, Christina; Hertwig, Stefan; Wecke, Jörg; Appel, Bernd

doi:10.1128/aem.67.12.5634-5642.2001

Cited by 82 publications

(77 citation statements)

References 31 publications

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“…Yersinia strains and other enterobacterial strains were obtained from the Robert Koch Institute collection (27,44) Isolation of PY100, propagation, and plaque assay. Manure was centrifuged twice at 10,000 ϫ g, and the supernatant was passed through a 0.45-m-pore-size filter.…”

Section: Methodsmentioning

confidence: 99%

Broad-Host-Range Yersinia Phage PY100: Genome Sequence, Proteome Analysis of Virions, and DNA Packaging Strategy

et al. 2008

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PY100 is a lytic bacteriophage with a broad host range within the genus Yersinia. The phage forms plaques on strains of the three human pathogenic species Yersinia enterocolitica, Y. pseudotuberculosis, and Y. pestis at 37°C. PY100 was isolated from farm manure and intended to be used in phage therapy trials. PY100 has an icosahedral capsid containing double-stranded DNA and a contractile tail. The genome consists of 50,291 bp and is predicted to contain 93 open reading frames (ORFs). PY100 gene products were found to be homologous to the capsid proteins and proteins involved in DNA metabolism of the enterobacterial phage T1; PY100 tail proteins possess homologies to putative tail proteins of phage Aa⌽23 of Actinobacillus actinomycetemcomitans. In a proteome analysis of virion particles, 15 proteins of the head and tail structures were identified by mass spectrometry. The putative gene product of ORF2 of PY100 shows significant homology to the gene 3 product (small terminase subunit) of Salmonella phage P22 that is involved in packaging of the concatemeric phage DNA. The packaging mechanism of PY100 was analyzed by hybridization and sequence analysis of DNA isolated from virion particles. Newly replicated PY100 DNA is cut initially at a pac recognition site, which is located in the coding region of ORF2.

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Section: Methodsmentioning

confidence: 99%

Broad-Host-Range Yersinia Phage PY100: Genome Sequence, Proteome Analysis of Virions, and DNA Packaging Strategy

et al. 2008

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“…The recombinant plasmid was identified by hybridization studies aimed at isolating the biosynthetic genes of enterocoliticin, a phage-tail-like bacteriocin produced by strain 29930 (Strauch et al, 2001;Goelz et al, 2003). Sequencing of a 3?2 kb EcoRI fragment of the insert DNA of Cos100 revealed the presence of ORFs encoding putative products with significant homology to proteins of the mating pair formation complex of T4SSs.…”

Section: Resultsmentioning

confidence: 99%

“…The T4SS described in this study was discovered in Y. enterocolitica 29930, a biotype 1A strain that releases the phage-tail-like particle enterocoliticin into the medium, which acts as a bacteriocin on a number of pathogenic and apathogenic Yersinia strains and rapidly kills sensitive strains, probably by forming channels in the membrane (Strauch et al, 2001). The Yersinia conjugative system is encoded by a cryptic plasmid present in the bacteriocin producer.…”

Section: Introductionmentioning

confidence: 92%

A cryptic plasmid of Yersinia enterocolitica encodes a conjugative transfer system related to the regions of CloDF13 Mob and IncX Pil

et al. 2003

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Yersinia enterocolitica 29930 (biotype 1A; O : 7,8), the producing strain of the phage-tail-like bacteriocin enterocoliticin, possesses a plasmid-encoded conjugative type IV transfer system. The genes of the conjugative system were found by screening of a cosmid library constructed from total DNA of strain 29930. The cosmid Cos100 consists of the vector SuperCos1 and an insert DNA of 40 303 bp derived from a cryptic plasmid of strain 29930. The conjugative transfer system consists of genes encoding a DNA transfer and replication system (Dtr) with close relationship to the mob region of the mobilizable plasmid CloDF13 and a gene cluster encoding a mating pair formation system (Mpf) closely related to the Mpf system of the IncX plasmid R6K. However, a gene encoding a homologue of TaxB, the coupling protein of the IncX system, is missing. The whole transfer region has a size of approximately 17 kb. The recombinant plasmid Cos100 was shown to be transferable between Escherichia coli and Yersinia with transfer frequencies up to 0·1 transconjugants per donor. Mutations generated by inserting a tetracycline cassette into putative tri genes yielded a transfer-deficient phenotype. Conjugative transfer of the cryptic plasmid could not be demonstrated in the original host Y. enterocolitica 29930. However, a kanamycin-resistance-conferring derivative of the plasmid was successfully introduced into E. coli K-12 by transformation and was shown to be self-transmissible. Furthermore, Southern blot hybridization and PCR experiments were carried out to elucidate the distribution of the conjugative transfer system in Yersinia. In total, six Y. enterocolitica biotype 1A strains harbouring closely related systems on endogenous plasmids were identified.

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“…20 as described by Strauch et al (16). The two tested bacterial species grown in nutrient broth medium at 30 °C for 24 h. and the tested fungi grown in the broth sabouraud's medium at 28 °C for 96 h. The cells of the test organisms were collected during exponential phase of growth by centrifugation, washed three times with 0.9% (wt.…”

Section: Minimal Inhibitory Concentration (Mic)mentioning

confidence: 99%

Antimicrobial activities and mode of action of the selected novel thienopyrimidines derivatives 2-[2-(diphenylmethylene) hydrazino]-5-isopropyl-3-methylthieno [2, 3-d] pyrimidin-4-one

El-Batanony¹

2017

Period Biol

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Characterization of Enterocoliticin, a Phage Tail-Like Bacteriocin, and Its Effect on Pathogenic Yersinia enterocolitica Strains

Cited by 82 publications

References 31 publications

Broad-Host-Range Yersinia Phage PY100: Genome Sequence, Proteome Analysis of Virions, and DNA Packaging Strategy

Broad-Host-Range Yersinia Phage PY100: Genome Sequence, Proteome Analysis of Virions, and DNA Packaging Strategy

A cryptic plasmid of Yersinia enterocolitica encodes a conjugative transfer system related to the regions of CloDF13 Mob and IncX Pil

Antimicrobial activities and mode of action of the selected novel thienopyrimidines derivatives 2-[2-(diphenylmethylene) hydrazino]-5-isopropyl-3-methylthieno [2, 3-d] pyrimidin-4-one

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