aims to assess the antimicrobial activity of newly synthesized thienopyrimidines derivatives against some pathogens and to determine the mode of action of the most potent compound 2-[2-(diphenylmethylene) hydrazino]-5-isopropyl-3-methylthieno [2, 3-d] pyrimidin-4-one (compound no. 20).
Materials and methods: Newly synthesized thieno [2,3-d]
L-Asparaginase (EC 3.5.1.1) is produced from actinomycetes to avoid the hypersensitive effect of that produced from other bacteria. Streptomyces halstedii strain was isolated from Egyptian soil and produced L-asparaginase. The 55.2-fold purified enzyme obtained had a final specific activity of 2071.2 U/mg. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) showed one band with molecular weight of 100 KDa. The K m value was 0.1939 mM. The enzyme showed maximum activity at pH 8.0, at optimum temperature at 37°C. Ethylene diamine tetraacetic acid (EDTA) and metal ions such as Zn 2+ , Hg 2+ Cu 2+ and K + decreased the activity of the enzyme. Ions such as Ca 2+ and Fe 2+ did not affect the activity of the enzyme. The enzyme showed cytotoxic activity against Ehrlich ascites cells (EAC) in vitro. In vivo, it showed a significant reduction in malondialdehyde (MDA) levels as the end product of lipid peroxidation and a remarkable increase in activity of liver antioxidant enzymes, [superoxide dismutase (SOD), catalase (CAT)] and a reduction in tumor weight. In conclusion, L-asparaginase from S. halstedii showed anti-tumor activity and cytotoxic effect against cancer cell line in vitro and in vitro. The reduction of tumor size in albino mice may be attributed to the elevation of CAT and SOD activities as well as the diminishing of MDA.
Two field experiments were carried out during two successive seasons of 2015 and 2016, to study the growth response and yield as well as chemical composition of some legume plants vis., cowpea (Vigna unguiculata L. cv Kaha 1), common bean (Phaseolus vulgaris L. cv. Nebraska), peas (Pisum sativum L. cv. Master B) and fenugreek (Trigonella foenum-graecum L., cv. Giza 3) to inoculation with non-rhizobial endophytic bacteria, strains ,vis., Enterobacter sp.(strain No.1), Pseudomonas sp.(strain No.2) and Pseudomonas sp.(strain No.3 ) which were isolated from root nodules of Melilotus indicus (L.) All. Results showed that almost all treatments increased all vegetative growth parameters tested, vis., shoot minerals, nitrogen content and total carbohydrate and seeds minerals, total protein, total carbohydrate and total yield (seeds and green yield) of the four tested legumes. Further, the three bacterial strains used in this study showed high growth promoting activities. They gave high production of IAA and showed highly antagonistic activity against some phytopathogenic fungi. Therefore, the best results were obtained with treatments T2 for cowpea and fenugreek and T4 for common bean and pea. So, it could be recommended to use these bacterial strains as inoculants for cowpea, fenugreek, common bean and pea crop legumes.
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