Characterization of electrospun fibrous scaffold produced from Indian eri silk fibroin

Abstract

A scaffold, synthesized from bio-degradable polymers and Bombyxmori silk fibroin in the form of films and fibrous assemblies, has been used as the bio-material for in-vivo applications. In the present work, the scaffold was prepared from the fibroin of Indian eri silk via the electrospinning method. The diameter of the fibre produced was in the range of 300 to 900 nm. The scaffold was subjected to ethanol treatment to improve its dimensional stability, as there was the problem of curling and shrinking when it was treated with solutions used for the cell culture. The scaffold was characterized for surface, thermal and tensile properties. The dimensional stability of the scaffold improved and the porosity reduced, due to the treatment of the scaffold with ethanol. The average failure stress of the raw and ethanol treated scaffold was 2.34 MPa and 4.91 MPa respectively and the mean strain was 13.63 % and 7.91 % respectively. Bone marrow stromal cells were isolated from the bone marrow of Swiss albino mice, and cultured on the ethanol treated electrospun fibrous scaffold. Scanning electron microscopy of the culture was carried out to evaluate the attachment and growth of cells on the scaffold at different incubation periods. Mouse bone marrow stromal cells adhered and grew on the electrospun fibrous scaffold prepared from eri silk fibroin, and the cell density increased with increasing incubation periods.

Fabrication and characterization of Eri silk fibers-based sponges for biomedical application

“…The degummed fibers exhibited peaks centered at 1654 cm À1 , 1535 cm À1 , and 1240 cm À1 attributed to the typical dominant beta sheet along with turn and random coil conformation (Fig. 1C-I) [37][38][39][40][41]. When comparing the degummed fiber (Fig.…”

Structural and Thermal Properties of Ethiopian Eri and Mulberry Silk Fibres

“…In another research work, the semi-alkali-treated silk fibres were incorporated as a reinforcing agent, while a mixture of 20% maleic anhydride-grafted polypropylene (MAPP) and time commercial grade polypropylene (PP) was used as a matrix element [17]. Andiyappan et al found that eri silk is suitable for wound-dressing applications due to its biochemical composition and lower sericin content, which enhances cell attachment behaviour [18]. Similarly, Chiriac et al stated that silk fibre characteristics are interesting for medical applications such as the induction of no or only a weak immune response, low bacterial adherence, and inherent biodegradability, among other desirable attributes.…”

Characterization of Electrospun Silk Fibroin Scaffolds for Bone Tissue Engineering: A Review

“…The crystal structure of silk fibroin nanofibers was studied using an X-ray diffractometer with a LynxEye detector [67], a CuKα radiation source (wavelength λ = 0.1541 nm) operating at 40kV and 40 mA over diffraction angles measured from 2θ = 0-40 °, and an X'celerator counter [49], [69] at a scanning rate of 0.015 °/s [67], 0.040 °/s [49], 0.048 °/s [70]. These parameters serve to obtain an X-ray diffractogram [71].The crystallinity of the silk fibroin nanofibers and the variation in the percentage of -sheet content corresponding to the crystal structure due to enzymatic degradation were determined [49].…”

“…In addition, these peaks indicate the coexistence of crystalline and amorphous structures in the electrospun of silk fibroin nanofibers although they were treated with methanol [69]. The estimated percentage of crystallinity of the pure silk fibroin membrane was 22.24 % [47]; its increase (to 35.54 %) is attributed to methanol [47], [66], [70], [72].…”