Characterization of Echinococcus granulosus of Spanish origin by DNA restriction endonuclease analysis and Southern blot hybridization

Cuesta-Bandera, C.; McManus, Donald P.; Rishi, Arun K.

doi:10.1016/0020-7519(88)90050-1

Cited by 19 publications

(6 citation statements)

References 7 publications

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“…The data, therefore, confirm and extend earlier studies (reviewed by McManus & Smyth, 1986) on the morphology, developmental characteristics, chemical constitution and metabolism, isoenzymes and DNA composition of E. granulosus from sheep and horses which demonstrated the distinctiveness between, but uniformity within, the sheep/dog and horse/dog strains in the U.K. Furthermore, the results of the current and previous studies (Macpherson & McManus, 1982;Cuesta-Bandera et al 1988;Thompson & Lymbery, 1988) suggest that the sheep/dog strain found in the United Kingdom is cosmopolitan in its distribution and that it is genetically homogeneous in sheep in the Mediterranean region and other areas of Europe, in the middle east, in Kenya, Australasia and in South America. The above arguments do not preclude the possibility that more than one strain can infect the same host species or that a single strain can occur in more than one intermediate host.…”

Section: Discussionsupporting

confidence: 91%

“…It is now generally accepted that molecular techniques, such as restriction enzyme analysis and Southern hybridization or, especially, nucleotide sequencing, can provide a much more valid and discriminatory approach to the identification and characterization of parasite isolates than classical methods involving morphological, in vitro culture or more conventional biochemical criteria (Rishi & McManus, 1989). In previous publications from our group, fragments of the ribosomal RNA gene of Schistosoma mansoni (McManus & Simpson, 1985;McManus et al 1987;Cuesta-Bandera, McManus & Rishi, 1988) and DNA segments of E. granulosus (Rishi & McManus, 1987;Cuesta-Bandera et al 1988), cloned in plasmids, have proved of value as DNA probes for characterizing isolates of E. granulosus. The work presented here extends the application of such methods to distinguish between isolates of E. granulosus from different host species and from various geographical locations.…”

Section: Introductionmentioning

confidence: 99%

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Genetic heterogeneity withinEchinococcus granulosus: isolates from different hosts and geographical areas characterized with DNA probes

McManus

Rishi²

1989

Parasitology

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A segment of the ribosomal RNA gene of Schistosoma mansoni and a DNA fragment specific to Echinococcus granulosus, cloned in plasmids, have been used as DNA probes to assess the extent of genetic variability within E. granulosus and some distinct strains have been identified. The DNA analysis, involving restriction endonuclease digestion and Southern blot hybridization with the probes, did not demonstrate any significant genetic variation within the U.K. horse/dog or sheep/dog strains but confirmed the distinctiveness of the two strains shown in previous studies. The sheep/dog strain was shown to be cosmopolitan in its distribution and fertile bovine material originating from the United Kingdom, Kenya, Spain and India conformed to this strain by DNA hybridization. In contrast, cattle isolates from Holland produced markedly different DNA hybridization banding profiles indicating that cattle can harbour more than one strain of E. granulosus. Similarly, it was shown that goats can harbour two different strains of E. granulosus, the sheep/dog strain and a form which infects camels. The strain of E. granulosus infecting equines in Spain and Ireland is genetically identical to that infecting horses in the United Kingdom. There is also a different strain infecting pigs in Poland and Yugoslavia. This pig/dog strain appears to be very similar genetically to the forms of E. granulosus which use camels and goats as intermediate hosts and is similar, though not identical, to the variant infecting Dutch cattle. It has been shown that E. granulosus material, fixed for a prolonged period in ethanol, or lyophilized, is amenable to DNA analysis and that it is possible to characterize the DNA of a single adult worm.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Genetic heterogeneity withinEchinococcus granulosus: isolates from different hosts and geographical areas characterized with DNA probes

McManus

Rishi²

1989

Parasitology

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“…They concluded that their results show the validity of the in vitro vesicular culture technique for strain identification and characterization in E. granulosus. Besides, their results concur with results obtained in previous genetic analyses (Cuesta-Bandera, 1988;Cuesta-Bandera et al, 1988;Siles-Lucas et al, 1993, 1994, 1996. The coincidence between the development times and physiological characteristics found in the present study working with samples of sheep and cattle origin indicate that the strain is the same: the common sheep strain.…”

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confidence: 92%

Strain characterization ofEchinococcus granulosusprotoscoleces of cattle origin using thein vitrovesicular development

Elissondo

Dopchiz

et al. 2005

Parasite

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Summary :The aim of this work was to characterize the strain of protoscoleces of E. granulosus of cattle origin using the in vitro vesicular development. The in vitro development of these samples was compared to samples of sheep origin determined previously by genetic analyses as common sheep strain (G1). There were similarities between sheep and cattle samples not only in the time of microcysts formation, but also in the development process. Vesiculated protoscoleces and protoscoleces with posterior bladders appeared during the first week of incubation. After 14 days of culture, a laminated layer appeared like a fine membrane in one of the extremes of the protoscoleces. In the sheep samples, microcysts were observed between 19 and 20 days. In the cattle samples, microcysts appeared between 20 and 23 days. The coincidence between the development times and physiological characteristics found in the present study may indicate that the parasites from cattle and sheep were of the same strain. Résumé : CARACTÉRISATION DE SOUCHES DE PROTOSCOLEX D'ECHINOCOCCUS GRANULOSUS

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“…Subspecific variation is a well-recognized phenomenon in E. granulosus (Thompson, 1986;McManus & Smyth, 1986) and substantial DNA differences have been demonstrated between strains of this organism McManus, Simpson & Rishi, 1987;Cuesta-Bandera, McManus & Rishi, 1988). Subspecific variation has also been described for other taeniids, notably T. crassiceps (Chew, 1981) and T. taeniaeformis (Brandt & Sewell, 1981;Williams, Shearer & Ravitch, 1981), but evidence for genetic variation within T. solium has been fragmentary and somewhat contradictory.…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning of Taenia solium genomic DNA and characterization of taeniid cestodes by DNA analysis

Rishi

McManus

1988

Parasitology

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Total DNAs, isolated from a range of taeniid cestodes (Taenia solium, T. saginata, T. pisiformis, T. crassiceps, T. hydatigena, T. ovis, T. multiceps and T. taeniaeformis), have been subjected to restriction enzyme digestion, Southern transfer and hybridization analysis using cloned fragments of the ribosomal RNA gene of Schistosoma mansoni. Substantial inter-specific genetic differences have been revealed on the basis of characteristic hybridization patterns for each of the taeniid cestode species. Furthermore, a random genomic DNA library has been constructed in the vector plasmid pAT153 using DNA extracted from a pig isolate (Indian origin) of T. solium. A panel of taeniid cestode DNAs including DNA from Echinococcus granulosus, has been used in conjunction with hybridization and restriction enzyme analysis to identify in the library a single recombinant plasmid with a T. solium-specific insert (coded pTS10) and two recombinant plasmids with T. solium inserts having selective specificities for T. solium and T. ovis (coded pTS17) and T. solium, T. saginata, T. ovis and T. multiceps (coded pTS28). These recombinant plasmids and the cloned fragments of the ribosomal RNA gene of S. mansoni have been used in restriction endonuclease, Southern transfer and hybridization analysis to detect intra-specific genetic variation in cysticerci of T. solium from India, Mexico and Zimbabwe. In addition, pTS10 and pTS17 have been used in a simple dot-blot assay to distinguish T. solium from T. saginata.

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Characterization of Echinococcus granulosus of Spanish origin by DNA restriction endonuclease analysis and Southern blot hybridization

Cited by 19 publications

References 7 publications

Genetic heterogeneity withinEchinococcus granulosus: isolates from different hosts and geographical areas characterized with DNA probes

Genetic heterogeneity withinEchinococcus granulosus: isolates from different hosts and geographical areas characterized with DNA probes

Strain characterization ofEchinococcus granulosusprotoscoleces of cattle origin using thein vitrovesicular development

Molecular cloning of Taenia solium genomic DNA and characterization of taeniid cestodes by DNA analysis

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