Characterization of dwarf mutants and molecular mapping of a dwarf locus in soybean

Cheng, Wen; Gao, Jinshan; Feng, Xingxing; Shao, Qun; Yang, Suxin; Feng, Xianzhong

doi:10.1016/s2095-3119(15)61312-0

Cited by 13 publications

(12 citation statements)

References 45 publications

(48 reference statements)

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“…Hedou 12 and Williams 82 were obtained from the Chinese Academy of Agricultural Sciences, and the Gmilpa1 mutant was created in our laboratory (Cheng et al, 2016). Wild-type Arabidopsis (Arabidopsis thaliana) and the apc8 mutant (Zheng et al, 2011) were obtained from Dr. Binglian Zheng at Fudan University.…”

Section: Plant Materialsmentioning

confidence: 99%

GmILPA1, Encoding an anaphase-promoting complex-like Protein, affects Leaf Petiole Angle

et al. 2017

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Leaf petiole angle (LPA) is an important plant architectural trait that affects canopy coverage, photosynthetic efficiency, and ultimately productivity in many legume crops. However, the genetic basis underlying this trait remains unclear. Here, we report the identification, isolation, and functional characterization of (), a gene encoding an APC8-like protein, which is a subunit of the anaphase-promoting complex/cyclosome in soybean (). A gamma ray-induced deletion of a fragment involving the fourth exon of and its flanking sequences led to extension of the third exon and formation of, to our knowledge, a novel 3'UTR from intronic and intergenic sequences. Such changes are responsible for enlarged LPAs that are associated with reduced motor cell proliferation in the mutant. is mainly expressed in the basal cells of leaf primordia and appears to function by promoting cell growth and division of the pulvinus that is critical for its establishment. GmILPA1 directly interacts with GmAPC13a as part of the putative anaphase-promoting complex. exhibits variable expression levels among varieties with different degrees of LPAs, and expression levels are correlated with the degrees of the LPAs. Together, these observations revealed a genetic mechanism modulating the plant petiole angle that could pave the way for modifying soybean plant architecture with optimized petiole angles for enhanced yield potential.

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Section: Plant Materialsmentioning

confidence: 99%

GmILPA1, Encoding an anaphase-promoting complex-like Protein, affects Leaf Petiole Angle

et al. 2017

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“…Plant height is generally controlled by node number on the main stem and internode length. We also found that the significantly decreased cell length contributed to the shortened internode, resulting in the sharp decrease in plant height of dw compared to wild type, while other dwarf soybean mutants mainly exhibited a substantial change in node number, internode length, or both (Hwang et al 2015 ; Cheng et al 2016 ). Furthermore, we mapped the GmDW1 gene to a 460-kb region on Chr.…”

Section: Discussionmentioning

confidence: 68%

“…The dw mutant is quite different from a few other soybean dwarf mutants that have been intensively studied recently. Of them, the Gmdwarf1 mutant slightly responded to GA 3 treatment (Zhang et al 2014 ), while dwarfism of Gmdwf1 could not be rescued by GA 3 application (Cheng et al 2016 ). The FN dwarf mutant, screened from an FN-mutagenized M4 population of Williams 82, began to display abnormal plant height after the V2 stage (Hwang et al 2015 ), however, the dw mutant in our study showed a consistent dwarf phenotype from the cotyledon expansion stage to maturity.…”

Section: Discussionmentioning

confidence: 99%

“…However, d53 is a rice SL-insensitive mutant and the reduced height does not respond to exogenous SL treatment due to a gain-of-function mutation in the D53 protein, acting as a repressor of SL signaling (Zhou et al 2013 ). In soybean, increasing numbers of mutants causing dwarfing have been also identified; these mutants occur following ionizing (gamma rays and fast neutrons) radiation (Zhang et al 2014 ; Hwang et al 2015 ; Cheng et al 2016 ) and/or chemical mutagenesis-generated populations (Li et al 2017 ). However, except for the peroxidase-encoding gene underlying the del3 - 15 locus on chromosome 15 (Hwang et al 2015 ), few dwarf genes have been cloned and characterized to date.…”

Section: Introductionmentioning

confidence: 99%

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Identification of the dwarf gene GmDW1 in soybean (Glycine max L.) by combining mapping-by-sequencing and linkage analysis

Guo

Ou³

et al. 2018

Theor Appl Genet

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Key messageGmDW1 encodes an ent-kaurene synthase (KS) acting at the early step of the biosynthesis pathway for gibberellins (GAs) and regulates the development of plant height in soybean.AbstractPlant height is an important component of plant architecture, and significantly affects crop breeding practices and yield. Here, we report the characterization of an EMS-induced dwarf mutant (dw) of the soybean cultivar Zhongpin 661 (ZDD23893). The dw mutant displayed reduced plant height and shortened internodes, both of which were mainly attributed to the longitudinally decreased cell length. The bioactive GA1 (gibberellin A1) and GA4 (gibberellin A4) were not detectable in the stem of dw, and the dwarf phenotype could be rescued by treatment with exogenous GA3. Genetic analysis showed that the dwarf trait of dw was controlled by a recessive nuclear gene. By combining linkage analysis and mapping-by-sequencing, we mapped the GmDW1 gene to an approximately 460-kb region on chromosome (Chr.) 8, containing 36 annotated genes in the reference Willliams 82 genome. Of these genes, we identified two nonsynonymous single nucleotide polymorphisms (SNPs) that are present in the encoding regions of Gmdw1 and Glyma.08G165100 in dw, respectively. However, only the SNP mutation (T>A) at nucleotide 1224 in Gmdw1 cosegregated with the dwarf phenotype. GmDW1 encodes an ent-kaurene synthase, and was expressed in various tissues including root, stem, and leaf. Further phenotypic analysis of the allelic variations in soybean accessions strongly indicated that GmDW1 is responsible for the dwarf phenotype in dw. Our results provide important information for improving our understanding of the genetics of soybean plant height and crop breeding.Electronic supplementary materialThe online version of this article (10.1007/s00122-017-3044-8) contains supplementary material, which is available to authorized users.

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“…Twenty progeny of each M 2 line were sowed in the field for phenotyping during May to October 2014. The screened mutant plants were backcrossed for four generations to purify the genetic background from July 2014 to October 2016 in both greenhouse and filed as described before (Chen et al, 2016). One of pale green leaf mutant, named as Glycine max pale green leaf 1 ( Gmpgl1 ), was selected to mapping the mutation locus and function study.…”

Section: Methodsmentioning

confidence: 99%

GmPGL1, a Thiamine Thiazole Synthase, Is Required for the Biosynthesis of Thiamine in Soybean

Feng¹,

Yang²,

Tang³

et al. 2019

Front. Plant Sci.

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Thiamine is an essential cofactor in several enzymatic reactions for all living organisms. Animals cannot synthesize thiamine and depend on their diet. Enhancing the content of thiamine is one of the most important goals of plant breeding to solve the thiamine deficiency associated with the low-thiamin staple crops. In this study, a Glycine max pale green leaf 1 (Gmpgl1) mutant was isolated from the EMS mutagenized population of soybean cultivar, Williams 82. Map-based cloning of the GmPGL1 locus revealed a single nucleotide deletion at the 292th nucleotide residue of the first exon of Glyma.10g251500 gene in Gmpgl1 mutant plant, encoding a thiamine thiazole synthase. Total thiamine contents decreased in both seedlings and seeds of the Gmpgl1 mutant. Exogenous application of thiazole restored the pale green leaf phenotype of the mutant. The deficiency of thiamine in Gmpgl1 mutant led to reduced activities of the pyruvate dehydrogenase (PDH) and pyruvate decarboxylase (PDC), and decreased contents of six amino acids as compared to that in the wild type plants. These results revealed that GmPGL1 played an essential role in thiamine thiazole biosynthesis.

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Characterization of dwarf mutants and molecular mapping of a dwarf locus in soybean

Cited by 13 publications

References 45 publications

GmILPA1, Encoding an anaphase-promoting complex-like Protein, affects Leaf Petiole Angle

GmILPA1, Encoding an anaphase-promoting complex-like Protein, affects Leaf Petiole Angle

Identification of the dwarf gene GmDW1 in soybean (Glycine max L.) by combining mapping-by-sequencing and linkage analysis

GmPGL1, a Thiamine Thiazole Synthase, Is Required for the Biosynthesis of Thiamine in Soybean

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