Characterization of duplicated zebrafish <i>cyp19</i> genes

Chiang, Evelyn; Yan, Yi; Tong, Sok-Keng; Hsiao, Pei Hung; Guiguen, Yann; Postlethwait, John H.; Chung, Bon Chu

doi:10.1002/jez.1121

Cited by 76 publications

(45 citation statements)

References 16 publications

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“…Besides En1, differences in the expression pattern of Msx zebrafish paralogs and homologous genes of other vertebrates also suggest subfunctionalization of the zebrafish genes after duplication (Ekker et al, 1997). Similar conclusions can be drawn for hedgehog genes (Laforest et al, 1998), Bmp2 (Martinez-Barbera et al, 1997, the transcription factors mitfa and mitfb (Mellgren and Johnson, 2002;Altschmied et al, 2002), cyp19 (Chiang et al, 2001), GlyRalpha genes (Imboden et al, 2001), Notch and Pax6 (Lynch and Force, 2000a). Models such as the DDC model may explain the retention and functional divergence of duplicated genes.…”

Section: The Evolutionary Consequences Of An Ancient Fish-specific Gementioning

confidence: 67%

Are all fishes ancient polyploids?

Peer¹,

Taylor²,

Meyer³

2003

Genome Evolution

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Euteleost fishes seem to have more copies of many genes than their tetrapod relatives. Three different mechanisms could explain the origin of these 'extra' fish genes. The duplicates may have been produced during a fishspecific genome duplication event. A second explanation is an increased rate of independent gene duplications in fish. A third possibility is that after gene or genome duplication events in the common ancestor of fish and tetrapods, the latter lost more genes. These three hypotheses have been tested by phylogenetic tree reconstruction. Phylogenetic analyses of sequences from human, mouse, chicken, frog (Xenopus laevis), zebrafish (Danio rerio) and pufferfish (Takifugu rubripes) suggest that ray-finned fishes are likely to have undergone a whole genome duplication event between 200 and 450 million years ago. We also comment here on the evolutionary consequences of this ancient genome duplication.

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Section: The Evolutionary Consequences Of An Ancient Fish-specific Gementioning

confidence: 67%

Are all fishes ancient polyploids?

Peer¹,

Taylor²,

Meyer³

2003

Genome Evolution

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“…Most teleosts have at least two CYP19 genes (P450arom), which encode structurally and functionally distinct P450arom isozymes in ovary ( CYP19a /P450aromA) and brain ( CYP19b/ P450aromB) (Callard and Tchoudakova, 1997;Chiang et al ., 2001;Gelinas et al ., 1998;Kwon et al ., 2001). In this study, we isolated two forms of the aromatase cDNA from the goby, T. okinawae .…”

Section: Discussionmentioning

confidence: 99%

“…We confirmed that both isoforms have aromatization ability as is the case with goldfish (Tchoudakova et al ., 1998) and zebrafish (Chiang et al ., 2001).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Two Types of Cytochrome P450 Aromatase in the Serial-sex Changing Gobiid Fish, Trimma okinawae

Kobayashi

Nakamura

et al. 2004

Zoological Science

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-To investigate the role of estrogen in the serial-sex changing fish Trimma okinawae , we isolated complementary DNAs encoding two distinct cytochrome P450 aromatase isoforms from adult ovary and brain (termed P450aromA and P450aromB, respectively). Sequence and phylogenic analyses showed that the goby P450arom forms belong to two separate CYP19 subfamilies. Transient expression of these cDNAs in HEK293 cells caused conversion of exogenous testosterone to estradiol-17 b . RT-PCR showed that P450aromA was expressed in the brain, spleen, testis and ovary. P450aromB was expressed in the brain, liver, testis and ovary. In situ hybridization studies showed that P450aromA mRNA, but not P 450aromB mRNA, was present in both ovary and testis. Positive signals were restricted to granulosa cells of vitellogenic follicles and interstitial cells of mature testis. Ovarian expression of both P450arom genes during the spawning cycle was examined by quantitative real-time RT-PCR. P450aromA transcripts increased during vitellogenesis and decreased prior to spawning. In contrast, P450aromB transcripts were barely detectable and did not correlate with ovarian development. These findings suggest that P450aromA, but not P450aromB, is involved in regulating ovarian vitellogenesis in goby.

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“…The brain of adult fish is well knownfor its high aromatase activity (Pasmanik and Callard, 1985;Pasmanik et al, 1988) corresponding to the strong expression of the cyp19a1b gene, that encodes CYP19A1B (or aromatase B), the brain form of aromatase in fish (Chiang et al, 2001;Tchoudakova et al, 2001). Although the functional significance for this unique feature of the fish brain is not fully understood, the mechanisms underlying this high expression are starting to be well deciphered.…”

Section: Introductionmentioning

confidence: 99%