Characterization of Two Types of Cytochrome P450 Aromatase in the Serial-sex Changing Gobiid Fish, Trimma okinawae

Kobayashi, Yasuhisa; Kobayashi, Tohru; Nakamura, Masaru; Sunobe, Tomoki; Morrey, Craig E.; Suzuki, Norio; Nagahama, Yoshitaka

doi:10.2108/zsj.21.417

Cited by 49 publications

(25 citation statements)

References 37 publications

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“…The similar patterns of cyp19a1a expression during oogenesis were also observed in red seabream (Gen et al 2001) and gobiid fish (Kobayashi et al 2004). The increase of ovarian cyp19a1a expression during vitellogenesis is in agreement with the role of gonadal estrogen in stimulating the hepatic synthesis of vitellogenin in teleosts (Nagahama 1994).…”

Section: Discussionsupporting

confidence: 67%

Two cytochrome P450 aromatase genes in the hermaphrodite ricefield eel Monopterus albus: mRNA expression during ovarian development and sex change

Zhang

Yang

et al. 2008

Journal of Endocrinology

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Previously, the ricefield eel (Monopterus albus) was speculated to have only one cytochrome p450 aromatase gene. In this study, however, the cDNAs encoding two distinct cytochrome p450 aromatases, cyp19a1a and cyp19a1b, were isolated. The genomic organizations of both cyp19 genes were conserved when compared with other teleosts. Northern blot detected an abundant expression of cyp19a1a in the ovary, and cyp19a1b in the hypothalamus. RT-PCR coupled with Southern blot showed that cyp19a1a was expressed predominantly in the gonads of both sexes, with higher levels in the ovary than testis, while cyp19a1b was expressed in all the tissues examined in the male, but only in the brain and pituitary in the female. The levels of cyp19a1a mRNA in the ovary were increased significantly during vitellogenesis, but decreased significantly at mature stage. The levels of cyp19a1b mRNA in the brain and pituitary did not vary significantly during vitellogenesis. As ovarian development shifted from vitellogenesis to maturation, the levels of cyp19a1b mRNA was decreased significantly in the brain, but increased significantly in the pituitary. During natural sex change from female to male, the levels of cyp19a1a mRNA in the gonad were significantly decreased. The levels of cyp19a1b mRNA in the hypothalamus were significantly increased at the early intersexual phase, whereas the expression levels in the pituitary were significantly decreased at the intersexual phases. Taken together, these results showed a novel sexual dimorphism of cyp19a1b mRNA tissue distribution, and both CYP19 genes were associated with the ovarian development and natural sex change of the ricefield eel.

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Section: Discussionsupporting

confidence: 67%

Two cytochrome P450 aromatase genes in the hermaphrodite ricefield eel Monopterus albus: mRNA expression during ovarian development and sex change

Zhang

Yang

et al. 2008

Journal of Endocrinology

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“…In T. okinawae, the ovarian isoform mRNA was present in ovary and testis, and the ovarian isoform transcripts correlated with ovarian development, suggesting that the ovarian isoform, but not the brain isoform, is involved in the regulation of ovarian vitellogenesis in this species (Kobayashi et al 2004). Hence, if two aromatase isoforms are also present in G. erythrospilus, the results of our study suggest that fadrozole blocks the ovarian isoform in this species.…”

Section: Discussionmentioning

confidence: 52%

Aromatase pathway mediates sex change in each direction

et al. 2005

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The enzyme aromatase controls the androgen/oestrogen ratio by catalysing the irreversible conversion of testosterone into oestradiol (E 2 ). Therefore, the regulation of E 2 synthesis by aromatase is thought to be critical in sexual development and differentiation. Here, we demonstrate for the first time that experimental manipulation of E 2 levels via the aromatase pathway induces adult sex change in each direction in a hermaphroditic fish that naturally exhibits bidirectional sex change. Our results demonstrate that a single enzymatic pathway can regulate both female and male sexual differentiation, and that aromatase may be the key enzyme that transduces environmental, including social, cues to functional sex differentiation in species with environmental sex determination.

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“…Our previous PCR results suggested that there was approximately 700 times less CYP19A1 expression in male gonads compared to females, and there was actually more CYP19A2 in testis than CYP19A1 (Patel et al, 2006). CYP19A1 expression was also reported in goby testis but the regulatory mechanisms or the physiological significance of aromatase in the male gonad is currently unknown (Kobayashi et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Highest CYP19A1 mRNA expression was found in Stage III B zebrafish ovarian vitellogenic follicles (Goto- Kazeto et al, 2004;Rodriguez-Mari et al, 2005). Kobayashi and colleagues found CYP19A2 mRNA expression in goby thecal cells of previtellogenic follicles while the expression was more abundant in granulosa cells of vitellogenic follicles (Kobayashi et al, 2004). However, aromatase immunoreactivity was found in both cell types of female goby (Sunobe et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Local expression of CYP19A1 and CYP19A2 in developing and adult killifish (Fundulus heteroclitus)

Wang¹,

Willett²

2008

General and Comparative Endocrinology

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P450 aromatase (CYP19) is the terminal enzyme in the steroidogenic pathway and catalyzes the conversion of androgens to estrogens. Fundulus heteroclitus like other teleosts, express two CYP19 genes, CYP19A1 and CYP19A2. The expression of CYP19s in Fundulus was measured by in situ hybridization throughout development. In 90 dpf (day post-fertilization) fish and adult fish, CYP19A1 was expressed in the ooplasm of early stage I oocytes (primary growth stage). Expression of CYP19A1 was localized in the follicle cell layer of late stage I (previtellogenic stage) and stage II (vitellogenic stage) follicles, but by stage III (early maturational follicles) CYP19A1 expression was localized in the vitelline envelope. Overall, CYP19A1 oocyte membrane expression gradually declined from highest expression at late stage I to nondetectable levels by stage IV. Highest expression of CYP19A2 was detected in the brain including the hypothalamus from 4, 6, 8, 10, 14 dpf embryos, 90 dpf fry fish and adult fish brain. In females compared to males, there was higher CYP19A2 expression in olfactory bulb. In addition to the brain, there was strong CYP19A2 signal in adrenal/kidney cells in 6-14 dpf embryos. This work establishes the localization and constitutive expression of CYP19s in Fundulus which can then be compared with potential disruption of CYP19A1 and CYP19A2 expression and physiological consequences caused by environmental contaminants.

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Characterization of Two Types of Cytochrome P450 Aromatase in the Serial-sex Changing Gobiid Fish, Trimma okinawae

Cited by 49 publications

References 37 publications

Two cytochrome P450 aromatase genes in the hermaphrodite ricefield eel Monopterus albus: mRNA expression during ovarian development and sex change

Two cytochrome P450 aromatase genes in the hermaphrodite ricefield eel Monopterus albus: mRNA expression during ovarian development and sex change

Aromatase pathway mediates sex change in each direction

Local expression of CYP19A1 and CYP19A2 in developing and adult killifish (Fundulus heteroclitus)

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