Characterization of dry eye disease in a mouse model by optical coherence tomography and fluorescein staining

Messner, Alina; Fischak, Corinna; Pfister, Martín; Schützenberger, Kornelia; Garreis, Fabian; Paulsen, Friedrich; Stegmann, Hannes; Santos, Valentin Aranha dos; Garhöfer, Gerhard; Schmetterer, Leopold; Werkmeister, René M.

doi:10.1364/boe.10.004884

Cited by 8 publications

(3 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fluorescein dye (0.25%) and Lissamine Green B (3%) (Sigma-Aldrich, St. Louis, MO, USA) were used for corneal staining in B6 and NOD.B10.H2 b mice, respectively. The different dyes were selected for visualizing dry eyes in the two types of mice both having different levels of iris pigments, as reported in previous studies [29][30][31][32].…”

Section: Clinical Evaluation Of the Dry Eyementioning

confidence: 99%

Age-Dependent Distinct Distributions of Dendritic Cells in Autoimmune Dry Eye Murine Model

Jung

Ryu

Yoon

et al. 2021

Cells

View full text Add to dashboard Cite

We investigated whether aging-dependent changes in dendritic cell (DC) distributions are distinct in autoimmune dry eye compared with an aging-related murine model. Corneal staining and tear secretion were evaluated in young and aged C57BL/6 (B6) and NOD.B10.H2b mice (NOD). In the corneolimbus, lacrimal gland (LG), and mesenteric lymph node (MLN), CD11b− and CD11b+ DCs, CD103+ DCs and MHC-IIhi B cells were compared between young and aged B6 and NOD mice. With increased corneal staining, tear secretion decreased in both aged B6 and NOD mice (p < 0.001). In both aged B6 and NOD mice, the percentages of corneolimbal CD11b+ DCs were higher (p < 0.05) than those in young mice. While, the percentages of lymph nodal CD103+ DCs were higher in aged B6 and NOD mice (p < 0.05), the percentages of corneolimbal CD103+ DCs were only higher in aged NOD mice (p < 0.05). In aged NOD mice, the proportions of lacrimal glandial and lymph nodal MHC-IIhi B cells were also higher than those in young mice (p < 0.05). It indicates that corneolimbal or lacrimal glandial distribution of CD103+ DCs or MHC-IIhi B cells may be distinct in aged autoimmune dry eye models compared to those in aged immune competent murine models.

show abstract

Section: Clinical Evaluation Of the Dry Eyementioning

confidence: 99%

Age-Dependent Distinct Distributions of Dendritic Cells in Autoimmune Dry Eye Murine Model

Jung

Ryu

Yoon

et al. 2021

Cells

View full text Add to dashboard Cite

show abstract

“…In another article, the same research group administered sodium carboxymethylcellulose over the cornea in healthy and dry eye patients and successfully measured adhesiveness using FD-OCT [ 106 ]. Moreover, Messner and colleagues demonstrated dry eye disease in a mouse model using OCT and fluorescein staining [ 107 ]. They concluded that fluorescein staining can detect defects of the corneal surface, but OCT can also provide information on the depth of the defect and the close layers [ 107 ].…”

Section: Development Of 3d Cell Culture Modelsmentioning

confidence: 99%

“…Moreover, Messner and colleagues demonstrated dry eye disease in a mouse model using OCT and fluorescein staining [ 107 ]. They concluded that fluorescein staining can detect defects of the corneal surface, but OCT can also provide information on the depth of the defect and the close layers [ 107 ].…”

Section: Development Of 3d Cell Culture Modelsmentioning

confidence: 99%

The Communication between Ocular Surface and Nasal Epithelia in 3D Cell Culture Technology for Translational Research: A Narrative Review

Aydin

Dietrich

Witt

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

There is a lack of knowledge regarding the connection between the ocular and nasal epithelia. This narrative review focuses on conjunctival, corneal, ultrastructural corneal stroma, and nasal epithelia as well as an introduction into their interconnections. We describe in detail the morphology and physiology of the ocular surface, the nasolacrimal ducts, and the nasal cavity. This knowledge provides a basis for functional studies and the development of relevant cell culture models that can be used to investigate the pathogenesis of diseases related to these complex structures. Moreover, we also provide a state-of-the-art overview regarding the development of 3D culture models, which allow for addressing research questions in models resembling the in vivo situation. In particular, we give an overview of the current developments of corneal 3D and organoid models, as well as 3D cell culture models of epithelia with goblet cells (conjunctiva and nasal cavity). The benefits and shortcomings of these cell culture models are discussed. As examples for pathogens related to ocular and nasal epithelia, we discuss infections caused by adenovirus and measles virus. In addition to pathogens, also external triggers such as allergens can cause rhinoconjunctivitis. These diseases exemplify the interconnections between the ocular surface and nasal epithelia in a molecular and clinical context. With a final translational section on optical coherence tomography (OCT), we provide an overview about the applicability of this technique in basic research and clinical ophthalmology. The techniques presented herein will be instrumental in further elucidating the functional interrelations and crosstalk between ocular and nasal epithelia.

show abstract

Thermoresponsive in-situ gel containing hyaluronic acid and indomethacin for the treatment of corneal chemical burn

Castro

Reis

Fernandes

et al. 2023

International Journal of Pharmaceutics

View full text Add to dashboard Cite

Characterization of dry eye disease in a mouse model by optical coherence tomography and fluorescein staining

Cited by 8 publications

References 30 publications

Age-Dependent Distinct Distributions of Dendritic Cells in Autoimmune Dry Eye Murine Model

Age-Dependent Distinct Distributions of Dendritic Cells in Autoimmune Dry Eye Murine Model

The Communication between Ocular Surface and Nasal Epithelia in 3D Cell Culture Technology for Translational Research: A Narrative Review

Thermoresponsive in-situ gel containing hyaluronic acid and indomethacin for the treatment of corneal chemical burn

Contact Info

Product

Resources

About