Characterization of drug-resistant<i>Staphylococcus aureus</i>isolated from poultry processing plants in Western Australia

Bertolatti, Dean; O’Brien, Frances; Grubb, W.B.

doi:10.1080/0960312021000063322

Cited by 13 publications

(19 citation statements)

References 32 publications

(40 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…At the moment, 11 SCCmec types have been reported and are used for the typing of MRSA strains (11). It is suggested that extensive and, often, indiscriminate use of antimicrobial agents, in animal husbandry, might constitute a risk factor in creating an animal reservoir of antimicrobial-resistant bacteria (2,12). From this reservoir, resistant strains or resistance genes might spread to humans, via the food chain.…”

Section: Introductionmentioning

confidence: 99%

“…It is also a pathogen for many animal species, including intensively farmed foodproducing animals. Worldwide, the dramatic increase in the prevalence of antimicrobial-resistant S. aureus (2)(3)(4) is receiving widespread attention, due to multi-resistant strains, diminishing the usefulness of antibiotics in human medicine and, thereby, limiting therapeutic options (5). Staphylococci are some of the most common causes of infections in birds (6).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Characteristics of Methicillin Resistant Staphylococcus aureus Strains Isolated From Poultry in Iran

Rahimi

Karimi

2015

Arch Clin Infect Dis

View full text Add to dashboard Cite

Background: Staphylococci are some of the most common causes of infections in birds. Worldwide, the dramatic increase in the prevalence of antimicrobial-resistant Staphylococcus aureus (S. aureus) is receiving widespread attention, due to multi-resistant strains, diminishing the usefulness of antibiotics in human medicine and, thereby limiting therapeutic options. Objectives: In this study, we characterized the distribution and antibiotic resistance patterns of methicillin resistant S. aureus (MRSA) strains, isolated from lying hen farms in Karaj, Iran. The pulsed field gel electrophoresis patterns and the staphylococcus cassette chromosome mec (SCCmec) types were also determined. Materials and Methods: Over a period of 90 days (collected at days: 0, 45, 90) during 2013, nine samplings, consisting of swab samples and litter collection, were done from three poultry farms (three each) and a total of 55 MRSA isolates were isolated from chromogenic MRSA selective agar. The clonality of MRSA strains was determined using pulsed field gel electrophoresis (PFGE) and the diversity in the structure of SCCmec elements and also different ccr types was studied. Susceptibility to seventeen antibiotics was determined, using disc diffusion method, according to Clinical and Laboratory Standards Institute recommendation. Results: Out of the 55 MRSA strains, all isolates were at least resistant to penicillin, 58% showed resistance to erythromycin and 55% were resistant to ciprofloxacin. On the other hand, all isolates showed susceptibility to vancomycin, quinuprostin-dalfopristin, linezolid, fusidic acid, nitrofurantoin and minocycline. The results of PFGE showed diverse pulsotypes, consisting of 13 common types and 18 single types, with seven common PFGE types, which were found among the MRSA strains, isolated from different farms, suggestive of an epidemiological link. Moreover, 67% of MRSA isolates shared SCCmec type III and showed type 3 ccr, indicating the hospital origin of the strains. Conclusions:The results of this study illustrated the persistence of resistant bacteria in the environment, and highlight the reservoir of resistance, associated with use of antibiotics, as feed additive in poultry production.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characteristics of Methicillin Resistant Staphylococcus aureus Strains Isolated From Poultry in Iran

Rahimi

Karimi

2015

Arch Clin Infect Dis

View full text Add to dashboard Cite

show abstract

“…Esses antimicrobianos são largamente utilizados no tratamento e controle de infecções bacterianas nas granjas. Além disso, são administrados há muitos anos, não só para controlar e prevenir a doença, mas também para promover o crescimento e melhorar a eficiência alimentar das aves (Geonaras & Holy 2001, Bertolatti et al 2003. Dessa forma, a resistência de cepas produtoras de biofilme pode interferir na resposta terapêutica de aves que apresentam sinais clínicos de doença respiratória.…”

Section: Discussionunclassified

“…No Brasil, pouco se sabe sobre os impactos do uso de antibióticos na avicultura comercial e os danos gerados à saúde animal e pública. A ocorrência de resistência antimicrobiana entre isolados recentes e antigos indica que a resistência aos antimicrobianos em estafilococos de origem aviária aumentou ao longo do tempo em indústrias avícolas da Bélgica e isto pode ser devido ao uso frequente de agentes antimicrobianos na criação de aves (Geonaras & Holy 2001, Bertolatti et al 2003.…”

Section: Discussionunclassified

Perfil de resistência a antimicrobianos de Staphylococcus spp. isolados de frangos de corte e poedeiras comerciais no estado de Pernambuco

et al. 2011

View full text Add to dashboard Cite

Este estudo foi realizado com o objetivo de pesquisar Staphylococcus spp. de frangos de corte sadios e frangos de corte e poedeiras comerciais que apresentassem sinais clínicos respiratórios. Foram colhidos swabs dos seios infra-orbitários de 55 frangos de corte sadios, 35 com sinais respiratórios, e 30 poedeiras comerciais também com sinais respiratórios. Cada amostra foi composta por um "pool" de cinco aves, totalizando 24 amostras coletadas de 24 granjas comerciais. Para o isolamento foi utilizado o exame bacteriológico, com posterior avaliação das características morfológicas, tintoriais e bioquímica para determinação da espécie. Verificou-se a produção de hemólise, formação de biofilme em ágar Vermelho Congo (ACR), detecção do gene mecA pela PCR e avaliação da suscetibilidade a 13 drogas antimicrobianas. Das 24 amostras processadas, foram isolados 16 Staphylococcus, cinco isolados foram coagulase-positiva (SCP) e 11 coagulase-negativa (SCN), e nos testes de hemólise e formação de biofilme, três isolados apresentaram-se hemolíticos e seis foram positivos, respectivamente. Na avaliação por meio da PCR, para detecção do gene mecA todos os isolados apresentaram resultados negativo. Observaram-se que 15 isolados foram resistentes a cinco ou mais antibióticos, e que as drogas associadas apresentaram melhor perfil de sensibilidade. A resistência a antimicrobianos e cepas produtoras de biofilme podem interferir na resposta terapêutica de aves que apresentam sinais clínicos.

show abstract

“…It is known that infections due to antibiotic-resistant strains of Staph. aureus are increasingly serious problem clinically and, since antibiotic exposure in food-animal species may lead to antibiotic-resistant bacteria, it is possible that processed poultry may constitute a reservoir for disseminating antibiotic-resistance into the community (Bertolatti et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Molecular Study on Antibiotic Resistance of Staph. aureus Isolated from Chicken Meat and its Products

Aa¹

2017

AJVS

View full text Add to dashboard Cite

Key words:Antibiotic Resistance, Staph. Aureus, Chicken meatIn this study, a total of 147 random samples from chicken meat, chicken liver, minced chicken meat and chicken luncheon samples were collected from different markets. Staphylococcus.aureus was isolated from these products at a percentage of 11.4 % , 9%, 16.2% and 26.1 % respectively.The sensitivity of Staph.aureus isolates to antimicrobial agents indicated that the isolates were sensitive to amikacin followed by gentamycin and amoxicillin with a percentage of 52 %, 48% and 30%, respectively. On the other hand, 100% of isolates were resistant to methicillin followed bypenicillin, doxycycline, Ampicillin, ciprofloxacin, and sulpha-methoxazole with a percentage of 96.3%, 96.3%, 93%, 89%, and 81%, respectively.The multidrug resistance (MAR) index values were ranged between 0.33 and 1. All the isolates showed resistance to at least 3 out 9 antibacterial agents tested. Six Staph.aureus isolates were selected (4 resistant to penicillin and 2 sensitive to penicillin) and screened for plasmid. The results revealed that the 4 resistant isolates were harbored plasmid with molecular size ranging from 18 and 22kbp. The 4 resistant isolates were divided to 2 groups (the first group was subcultured 5 times with sublethal dose of penicillin and the second group was subcultured 5 times in the absence of penicillin) and screened for plasmid. It was found that the 2 isolates which subjected to 5 times repeated subculture with sublethal dose of penicillin maintained their plasmids, while the 2 isolates which subjected to 5 times repeated subculture in the absence of penicillin lost their plasmids.PCR technique was used as confirmatory tool for detection of mecA gene. Eight isolates of Staph .aureus were randomly selected for detection ofmecA gene using specific primers. Five out of eight (62.5 %) isolates were positive for mecA gene. It could be concluded that the drug of choice to Staph. aureuswas amikacin. All isolates had MAR index of 0.3 and above. The resistance of Staph.aureus to methicillin was higher. The presence of the antibiotic is important for maintenance of plasmid DNA.

show abstract

Characterization of drug-resistantStaphylococcus aureusisolated from poultry processing plants in Western Australia

Cited by 13 publications

References 32 publications

Characteristics of Methicillin Resistant Staphylococcus aureus Strains Isolated From Poultry in Iran

Characteristics of Methicillin Resistant Staphylococcus aureus Strains Isolated From Poultry in Iran

Perfil de resistência a antimicrobianos de Staphylococcus spp. isolados de frangos de corte e poedeiras comerciais no estado de Pernambuco

Molecular Study on Antibiotic Resistance of Staph. aureus Isolated from Chicken Meat and its Products

Contact Info

Product

Resources

About