Characterization of dominant giant rod-shaped magnetotactic bacteria from a low tide zone of the China Sea

A species of magnetotactic deltaproteobacterium was detected at the highest abundance during an algal bloom

“…The use of micromanipulation in studies involving genome sequences of MTB has been reported previously (Chen, et al 2015b, Jogler, et al 2011, Teng, et al 2018a, Teng, et al 2018b. WGA of the MTB was performed using the illustra Single Cell GenomiPhi DNA Amplification Kit (GE29-1080-39; Sigma, United States) following the manufacturer's instructions, with a 2.5-h amplification (Teng, et al 2018a, Teng, et al 2018b. PCR amplification (Mastercycler; Eppendorf, German) was conducted using the bacterial universal primers 27f and 1492r (Lane 1991) (Sangon Biotech, Shanghai, China).…”

“…For phylogenetic analysis of the MTB we combined micromanipulation with whole genome amplification (WGA) and polymerase chain reaction (PCR) amplification of the 16S rRNA gene. The use of micromanipulation in studies involving genome sequences of MTB has been reported previously (Chen, et al 2015b, Jogler, et al 2011, Teng, et al 2018a, Teng, et al 2018b. WGA of the MTB was performed using the illustra Single Cell GenomiPhi DNA Amplification Kit (GE29-1080-39; Sigma, United States) following the manufacturer's instructions, with a 2.5-h amplification (Teng, et al 2018a, Teng, et al 2018b.…”

Diversity and Characterization of Multicellular Magnetotactic Prokaryotes From Coral Reef Habitats of the Paracel Islands, South China Sea

“…For phylogenetic analysis of MMPs from the coral reef habitats, we combined micromanipulation with WGA, followed by polymerase chain reaction (PCR) amplification of the 16S rRNA gene. The procedures of using micromanipulation for identification of MTB and MMPs genome sequencing have been described previously ( Jogler et al, 2011 ; Kolinko et al, 2012 ; Zhang et al, 2014 ; Chen et al, 2015 , 2016 ; Liu et al, 2017 , 2018 ; Teng et al, 2017 ) and WGA of the cells of MMPs was carried out according to the provided guidelines of the illustra TM Single Cell GenomiPhi TM DNA Amplification Kit (GE29-1080-39; Sigma, United States), with an amplification time of 2.5 h. The bacteria-specific primers 27f and 1492r (Sangon Biotech, Shanghai, China) were used for the PCR ( Frank et al, 2008 ), which was performed using a Mastercycler (Eppendorf, German). The resulting PCR products were cloned into the pMD18-T vector (Takara, Dalian, China) and transformed into competent E. coli TOP10 cells.…”

“…The resulting PCR products were cloned into the pMD18-T vector (Takara, Dalian, China) and transformed into competent E. coli TOP10 cells. The clones were randomly selected for sequencing, which was carried out by Nanjing Genscript Biotechnology (Nanjing, China) ( Teng et al, 2017 ).…”

“…All the sequences were aligned using CLUSTAL W multiple alignment software, and sequence identities were calculated using the BIOEDIT software. A phylogenetic tree was constructed using the neighbor-joining method in MEGA 6.0, and bootstrap values were calculated from 1000 replicates ( Teng et al, 2017 ). The sequences were submitted in the GenBank database under accession numbers KY21895 – KY21900 .…”