Characterization of dihydroflavonol 4-reductase (DFR) genes and their association with cold and freezing stress in Brassica rapa

Ahmed, Nasar Uddin; Park, Jong-In; Jung, Hee-Jeong; Yang, Tae-Jin; Hur, Yoonkang; Nou, Ill–Sup

doi:10.1016/j.gene.2014.08.013

Cited by 101 publications

(76 citation statements)

References 40 publications

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“…In addition, TaDFR transcript levels significantly increased in the coleoptiles in response to low temperature, drought, high salt levels, hormones, sugars, and high levels of white light and UV, which is consistent with previous findings (Helariutta et al 1993;Gollop et al 2002;Himi and Xie et al 2004;Ahmed et al 2009;Das et al 2012;Ahmed et al 2014;Ma et al 2014;Mori et al 2014). Anthocyanin coloring of the pericarp or aleurone layer can be used as a marker to identify wheat with specific nutritional qualities, to determine the rate of out-crossing of self-pollinating wheat, and to develop hybrid wheat systems (Morrison et al 2004).…”

Section: Discussionsupporting

confidence: 90%

Overexpressing the wheat dihydroflavonol 4-reductase gene TaDFR increases anthocyanin accumulation in an Arabidopsis dfr mutant

et al. 2015

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Section: Discussionsupporting

confidence: 90%

Overexpressing the wheat dihydroflavonol 4-reductase gene TaDFR increases anthocyanin accumulation in an Arabidopsis dfr mutant

et al. 2015

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“…In characterizing DFR genes, Ahmed et al found that these genes are associated with cold and freezing stresses in B. rapa [39]. Guo et al .…”

Section: Discussionmentioning

confidence: 99%

Genetics and fine mapping of a purple leaf gene, BoPr, in ornamental kale (Brassica oleracea L. var. acephala)

Gao

Han

et al. 2017

BMC Genomics

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BackgroundDue to its variegated and colorful leaves, ornamental kale (Brassica oleracea L. var. acephala) has become a popular ornamental plant. In this study, we report the fine mapping and analysis of a candidate purple leaf gene using a backcross population and an F2 population derived from two parental lines: W1827 (with white leaves) and P1835 (with purple leaves).ResultsGenetic analysis indicated that the purple leaf trait is controlled by a single dominant gene, which we named BoPr. Using markers developed based on the reference genome ‘02–12’, the BoPr gene was preliminarily mapped to a 280-kb interval of chromosome C09, with flanking markers M17 and BoID4714 at genetic distances of 4.3 cM and 1.5 cM, respectively. The recombination rate within this interval is almost 12 times higher than the usual level, which could be caused by assembly error for reference genome ‘02–12’ at this interval. Primers were designed based on ‘TO1000’, another B. oleracea reference genome. Among the newly designed InDel markers, BRID485 and BRID490 were found to be the closest to BoPr, flanking the gene at genetic distances of 0.1 cM and 0.2 cM, respectively; the interval between the two markers is 44.8 kb (reference genome ‘TO1000’). Seven annotated genes are located within the 44.8 kb genomic region, of which only Bo9g058630 shows high homology to AT5G42800 (dihydroflavonol reductase), which was identified as a candidate gene for BoPr. Blast analysis revealed that this 44.8 kb interval is located on an unanchored scaffold (Scaffold000035_P2) of ‘02–12’, confirming the existence of assembly error at the interval between M17 and BoID4714 for reference genome ‘02–12’.ConclusionsThis study identified a candidate gene for BoPr and lays a foundation for the cloning and functional analysis of this gene.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3613-x) contains supplementary material, which is available to authorized users.

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“…These compounds are beneficial for human health and are an important group of pigments that colour the leaves and flowers of many plants [5]. Anthocyanins are also involved in biotic and abiotic stress defence responses [6,7]. It is vital to elucidate the flavonoid biosynthesis and regulatory pathways in SYQ.…”

Section: Introductionmentioning

confidence: 99%

Integrated analysis of the transcriptome and metabolome of purple and green leaves of Tetrastigma hemsleyanum reveals gene expression patterns involved in anthocyanin biosynthesis

Yan

Liu

Zhu³

et al. 2020

PLoS ONE

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To gain better insight into the regulatory networks of anthocyanin biosynthesis, an integrated analysis of the metabolome and transcriptome in purple and green leaves of Tetrastigma hemsleyanum was conducted. Transcript and metabolite profiles were archived by RNA-sequencing data analysis and LC-ESI-MS/MS, respectively. There were 209 metabolites and 4211 transcripts that were differentially expressed between purple and green leaves. Correlation tests of anthocyanin contents and transcriptional changes showed 141 significant correlations (Pearson correlation coefficient >0.8) between 16 compounds and 14 transcripts involved in the anthocyanin biosynthesis pathway. Some novel genes and metabolites were discovered as potential candidate targets for the improvement of anthocyanin content and superior cultivars. OPEN ACCESS Citation: Yan J, Qian L, Zhu W, Qiu J, Lu Q, Wang X, et al. (2020) Integrated analysis of the transcriptome and metabolome of purple and green leaves of Tetrastigma hemsleyanum reveals gene expression patterns involved in anthocyanin biosynthesis. PLoS ONE 15(3): e0230154. https://doi.org/10.

show abstract

Characterization of dihydroflavonol 4-reductase (DFR) genes and their association with cold and freezing stress in Brassica rapa

Cited by 101 publications

References 40 publications

Overexpressing the wheat dihydroflavonol 4-reductase gene TaDFR increases anthocyanin accumulation in an Arabidopsis dfr mutant

Overexpressing the wheat dihydroflavonol 4-reductase gene TaDFR increases anthocyanin accumulation in an Arabidopsis dfr mutant

Genetics and fine mapping of a purple leaf gene, BoPr, in ornamental kale (Brassica oleracea L. var. acephala)

Integrated analysis of the transcriptome and metabolome of purple and green leaves of Tetrastigma hemsleyanum reveals gene expression patterns involved in anthocyanin biosynthesis

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