Characterization of dental follicle cells in developing mouse molar

Hou, Lein-Tuan; Liu, Cheing-Meei; Chen, Yi-Jane; Wong, Man-Ying; Chen, Kun-Chee; Chen, Jinkun; Thomas, Huw F.

doi:10.1016/s0003-9969(99)00033-3

Cited by 24 publications

(15 citation statements)

References 51 publications

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“…Regardless, a more detailed study is required to elucidate the precise mechanism of VEGF on HDFC proliferation. Previous studies based on the rodentine dental follicle show that the dental follicle cells could exhibit partial characteristics of a mineralized tissue-forming phenotype [37] . Recently, HDFC have been reported to share some of the biochemical features of osteoblastic phenotypes.…”

Section: Discussionmentioning

confidence: 98%

Expression of vascular endothelial growth factor in cultured human dental follicle cells and its biological roles

Chen

Qian

et al. 2007

Acta Pharmacologica Sinica

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Aim: To investigate the expression of vascular endothelial growth factor (VEGF) in cultured human dental follicle cells (HDFC), and to examine the roles of VEGF in the proliferation, differentiation, and apoptosis of HDFC in vitro. Methods: Immunocytochemistry, ELISA, and RT-PCR were used to detect the expression and transcription of VEGF in cultured HDFC. The dose-dependent and the timecourse effect of VEGF on cell proliferation and alkaline phosphatase (ALP) activity in cultured HDFC were determined by MTT assay and colorimetric ALP assay, respectively. The effect of specific mitogen-activated protein kinase (MAPK) inhibitors (PD98059 and U0126) on the VEGF-mediated HDFC proliferation was also determined by MTT assay. The effect of VEGF on HDFC apoptosis was measured by flow cytometry. Results: VEGF was transcribed and expressed in cultured HDFC. VEGF at 10-300 µg/L significantly increased HDFC proliferation and ALP activity compared to the control. Following 1, 3, 5, or 7 d of stimulation, VEGF induced a significant increase in HDFC proliferation compared with the corresponding control, while VEGF was effective at increasing ALP activity at the incubation time point of 3, 5, or 7 d. PD98059 and U0126 could attenuate the VEGF-mediated HDFC proliferation. Fewer apoptotic cells were observed in the VEGF-treated groups compared to the controls, although the difference was not statistically significant. Conclusion: VEGF is expressed in cultured HDFC, and at a proper concentration range can stimulate HDFC proliferation, induce HDFC to differentiate in a "cementoblast/osteoblast" pathway and protect HDFC from apoptosis. The MAPK signaling pathway might be involved in the VEGF-mediated HDFC proliferation.

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Section: Discussionmentioning

confidence: 98%

Expression of vascular endothelial growth factor in cultured human dental follicle cells and its biological roles

Chen

Qian

et al. 2007

Acta Pharmacologica Sinica

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“…There have been a number of studies in support of the suitability of the DF for tissue regeneration on the basis of its capability to differentiate into cementum, periodontal ligament, and alveolar bone following stimulation with growth factors and extracellular matrix proteins (33,36). As discussed above, the DF's capacity to regenerate is probably not due to stem cells but rather an accomplishment of progenitors that may either be induced to commit to certain lineages or stimulated to pursue already precommitted pathways in hterogenous cell populations.…”

Section: Discussionmentioning

confidence: 99%

Dental Follicle Progenitor Cell Heterogeneity in the Developing Mouse Periodontium

Luan

Ito

Dangaria

et al. 2006

Stem Cells and Development

135

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As a developmental precursor for diverse periodontal tissues, the dental follicle (DF) harbors great promise for periodontal tissue regeneration. However, development of optimal therapy awaits the answer to a key question that impinges on many issues in development-Do adult progenitor tissues form a homogeneous cell population that differentiates into target tissues when they arrive at the site, or they contain heterogeneous cell populations that are committed to specific fates? To address the homogeneity/heterogeneity question, we analyzed differentiation pathways and markers in several cloned DF cell lines. Our studies revealed that each of our cloned DF lines featured remarkably unique characteristics, indicative of a separate and distinct lineage. One line, DF1, was high in proliferative activity but did not display any mineralization behavior, suggesting that it might be related to a periodontal ligament-type lineage. DF2 was similar to DF1, but featured remarkably high alkaline phosphatase activity indicative of a highly undifferentiated state. DF3 matched the mineralization characteristics of a same stage alveolar bone line AB1 in terms of gene expression and von Kossa staining, indicating that DF3 might be of cementoblastic or alveolar bone osteoblastic lineage. To verify the multilineage potential of the DF for purposes of tissue engineering, a series of differentiation induction experiments was conducted. For identification purposes, characteristics of these heterogeneous follicular progenitor cells were compared with follicle components in tissue sections of the postnatal developing periodontium. The presence of heterogeneous cell populations in the DF mirrors individual developmental pathways in the formation of the dental integument. The profound cellular heterogeneity of the DF as an adult progenitor for tissue regeneration also suggests that heterogeneous cellular constituents might play as much of a role in tissue regeneration as the inducible characteristics of individual lineages might do.

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“…The follicle was dissected from the fi rst mandibular molar and digested with a mixture of collagenase and trypsin to isolate cells. Follicle cells were immortalized, using SV40, and found to be positive for expression of follicle cell markers (colony-stimulating factor 1, epidermal growth factor, epidermal growth factor receptor, and others) [Wise et al, 1992;Hou et al, 1999]. PDL cells and cementoblasts were similarly isolated from mice at developmental stage day 41 from vaginal plug date.…”

Section: Methodsmentioning

confidence: 99%

Defining the Roots of Cementum Formation

Popowics

Foster

Swanson

et al. 2005

Cells Tissues Organs

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Significant progress has been seen in research aimed at regeneration of the disease-damaged periodontium. Our own strategy has been to approach periodontal tissue development (i.e. root, cementum, periodontal ligament, and bone) as a source for the identification of key regulators of cellular processes that may be applicable to periodontal tissue repair. Specifically, enamel-like molecules, bone morphogenetic proteins (BMPs), and phosphates have been investigated for their role in altering gene expression and cell functions in follicle cells, periodontal ligament cells, and cementoblasts. Amelogenin, leucine-rich amelogenin peptide, and tyrosine-rich amelogenin peptide have been found to similarly affect cementoblast gene expression and cementoblast-mediated mineralization in vitro; however, these enamel-like factors do not increase cell proliferation as has been observed in cells treated with Emdogain^® (Biora AB, Malmö, Sweden), an enamel matrix derivative. BMP-2 has been found to promote differentiation of follicle cells into a cementoblast/osteoblast phenotype, and BMP-3 is being investigated as a negative regulator of mineralization. The increased ratio of phosphate to pyrophosphate in the local region during root development has been found to significantly enhance the extent of cementum formation in animal models. Furthermore, phosphate has been identified as a regulator of cementoblast SIBLING (small integrin-binding ligand N-linked glycoprotein) gene expression in vitro. These investigations of candidate factors for periodontal regeneration have uncovered mechanisms regulating gene expression and cell function in cells controlling the behavior of periodontal tissues (i.e. follicle cells, periodontal cells, and cementoblasts) and offer new directions to consider for clinical repair of periodontal defects.

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Characterization of dental follicle cells in developing mouse molar

Cited by 24 publications

References 51 publications

Expression of vascular endothelial growth factor in cultured human dental follicle cells and its biological roles

Expression of vascular endothelial growth factor in cultured human dental follicle cells and its biological roles

Dental Follicle Progenitor Cell Heterogeneity in the Developing Mouse Periodontium

Defining the Roots of Cementum Formation

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