Characterization of culturable gut bacterial isolates from wild population of melon fruit fly (Bactrocera cucurbitae) and assessing their attractancy potential for sustainable pest management

Mishra, Maneesh; Sharma, Kirti; Subramanian, S.

doi:10.1007/s12600-018-0694-2

Cited by 8 publications

(11 citation statements)

References 38 publications

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“…The bacterial genus Ochrobactrum, which in our study showed higher abundance in Z. cucurbitae in a number of interspecific pairwise comparisons, has often been reported as a plant endophyte of Cucurbitaceae (Weller et al, 2006;Akbaba and Ozaktan, 2018) and described in a number of cucurbit feeder fruit flies including Z. cucurbitae (Mishra and Sharma, 2018), Z. tau (pumpkin fly) (Khan et al, 2014;Prabhakar et al, 2013;Luo et al, 2018), and in the polyphagous B. tryoni (Jessup and Mccarthy, 1993). Similarly, the bacterial genus Rahnella which we consistently found in higher abundances in the gut microbiome of C. podocarpi has been reported in different species of bark beetles (Lacey et al, 2007;Vasanthakumar et al, 2009;Brady et al, 2014;Hernández-García et al, 2017), many of which feed on bark of coniferous trees.…”

Section: Discussionsupporting

confidence: 64%

Comparative Microbiomics of Tephritid Frugivorous Pests (Diptera: Tephritidae) From the Field: A Tale of High Variability Across and Within Species

et al. 2020

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The family Tephritidae includes some of the most notorious insect pests of agricultural and horticultural crops in tropical and subtropical regions. Despite the interest in the study of their gut microbiome, our present knowledge is largely based on the analysis of laboratory strains. In this study, we present a first comparative analysis of the gut microbiome profiles of field populations of ten African and Mediterranean tephritid pests. For each species, third instar larvae were sampled from different locations and host fruits and compared using 16S rRNA amplicon sequencing and a multi-factorial sampling design. We observed considerable variation in gut microbiome diversity and composition both between and within fruit fly species. A "core" microbiome, shared across all targeted species, could only be identified at most at family level (Enterobacteriaceae). At genus level only a few bacterial genera (Klebsiella, Enterobacter, and Bacillus) were present in most, but not all, samples, with high variability in their relative abundance. Higher relative abundances were found for seven bacterial genera in five of the fruit fly species considered. These were Erwinia in Bactrocera oleae, Lactococcus in B. zonata, Providencia in Ceratitis flexuosa, Klebsiella, and Rahnella in C. podocarpi and Acetobacter and Serratia in C. rosa. With the possible exception of C. capitata and B. dorsalis (the two most polyphagous species considered) we could not detect obvious relationships between fruit fly dietary breadth and microbiome diversity or abundance patterns. Similarly, our results did not suggest straightforward differences between the microbiome profiles of species belonging to Ceratitis and the closely related Bactrocera/Zeugodacus. These results provide a first comparative analysis of the gut microbiomes of field populations of multiple economically relevant tephritids and provide base line information for future studies that will further investigate the possible functional role of the observed associations.

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Section: Discussionsupporting

confidence: 64%

Comparative Microbiomics of Tephritid Frugivorous Pests (Diptera: Tephritidae) From the Field: A Tale of High Variability Across and Within Species

et al. 2020

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“…It was also among the dominant genera in wild populations from Thailand, and Malaysia, occurring in all populations with relative abundance values between 0–24.2% [ 16 ]. On the other hand, the bacteria were absent from Indian populations either when tested with high throughput sequencing [ 10 ] or culture dependent approaches [ 30 , 74 ]. Orbus bacteria were found in the closely related species from Chinese populations with 13.8–42.8% relative abundance [ 18 ], in B. dorsalis from Wuhan (0.04%) [ 15 ], but also in B. tryoni (Froggatt) (2%) and B. cacuminata (4.8%) from Australia [ 61 ].…”

Section: Discussionmentioning

confidence: 99%

“…However, four of them, were found in populations from India by culture dependent approaches, specifically Enterobacter (34.6%), Klebsiella (19.2%), Citrobacter (7.7%) and Providencia (7.7%) [ 30 ]. Isolates belonging to Enterobacter , Klebsiella , and Citrobacter were also found in another Indian population [ 74 ]. Klebsiella , Citrobacter , Pectobacterium and Enterobacter bacteria are mostly related to nitrogen fixation or exhibit pectinolytic activity.…”

Section: Discussionmentioning

confidence: 99%

New Insights on the Zeugodacus cucurbitae (Coquillett) Bacteriome

et al. 2021

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Various factors, including the insect host, diet, and surrounding ecosystem can shape the structure of the bacterial communities of insects. We have employed next generation, high-throughput sequencing of the 16S rRNA to characterize the bacteriome of wild Zeugodacus (Bactrocera) cucurbitae (Coquillett) flies from three regions of Bangladesh. The tested populations developed distinct bacterial communities with differences in bacterial composition, suggesting that geography has an impact on the fly bacteriome. The dominant bacteria belonged to the families Enterobacteriaceae, Dysgomonadaceae and Orbaceae, with the genera Dysgonomonas, Orbus and Citrobacter showing the highest relative abundance across populations. Network analysis indicated variable interactions between operational taxonomic units (OTUs), with cases of mutual exclusion and copresence. Certain bacterial genera with high relative abundance were also characterized by a high degree of interactions. Interestingly, genera with a low relative abundance like Shimwellia, Gilliamella, and Chishuiella were among those that showed abundant interactions, suggesting that they are also important components of the bacterial community. Such knowledge could help us identify ideal wild populations for domestication in the context of the sterile insect technique or similar biotechnological methods. Further characterization of this bacterial diversity with transcriptomic and metabolic approaches, could also reveal their specific role in Z. cucurbitae physiology.

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“…For example, both Rockett () and Poonam, Paily, and Balaraman () showed that volatiles produced by Bacillus species exhibited oviposition stimulation in Culex females . Furthermore, the melon fruit fly Bactrocera cucurbitae was attracted to broth cultures of Bacillus cereus (Mishra, Sharma, & Subramanian, ). Strikingly, one of the attractive Bacillus strains (ST18.16/043) was isolated from the hyperparasitoid D. aphidum , which complicates predictions regarding the adaptive value of responding to microbial volatiles.…”

Section: Discussionmentioning

confidence: 99%

Volatiles of bacteria associated with parasitoid habitats elicit distinct olfactory responses in an aphid parasitoid and its hyperparasitoid

et al. 2020

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1. To locate mating partners and essential resources such as food, oviposition sites and shelter, insects rely to a large extent on chemical cues. While most research has focused on cues derived from plants and insects, there is mounting evidence that indicates that micro-organisms emit volatile compounds that may play an important role in insect behaviour.2. In this study, we assessed how volatile compounds emitted by phylogenetically diverse bacteria affected the olfactory response of the primary parasitoid Aphidius colemani and one of its secondary parasitoids, Dendrocerus aphidum. Olfactory responses were evaluated for volatile blends emitted by bacteria isolated from diverse sources from the parasitoid's habitat, including aphids, aphid mummies and honeydew, and from the parasitoids themselves.3. Results revealed that A. colemani showed a wide variation in response to bacterial volatiles, ranging from significant attraction over no response to significant repellence. Our results further showed that the olfactory response of A. colemani to bacterial volatile emissions was different from that of D. aphidum. Gas chromatography-mass spectrometry analysis of the volatile blends revealed that bacterial strains repellent to A. colemani produced significantly higher amounts of esters, organic acids, aromatics and cycloalkanes than attractive strains. Strains repellent to D. aphidum produced significantly higher amounts of alcohols and ketones, whereas the strains attractive to D. aphidum produced higher amounts of the monoterpenes limonene, linalool and geraniol. 4. Overall, our results indicate that bacterial volatiles can have an important impact on insect olfactory responses, and should therefore be considered as an additional, so far often overlooked factor in studying multitrophic interactions between plants and insects. K E Y W O R D SAphidius colemani, chemical communication, Dendrocerus aphidum, microbial odour, multitrophic interactions, natural enemy, semiochemical, VOCs | 509 Functional Ecology GOELEN Et aL. Islam S. Sobhy https://orcid.org/0000-0003-4984-1823 Jetske G. de Boer https://orcid.org/0000-0002-3182-8543 Kevin J. Verstrepen https://orcid.org/0000-0002-3077-6219 Hans Jacquemyn https://orcid.org/0000-0001-9600-5794 Bart Lievens https://orcid.org/0000-0002-7698-6641 S U PP O RTI N G I N FO R M ATI O N Additional supporting information may be found online in the Supporting Information section. How to cite this article: Goelen T, Sobhy IS, Vanderaa C, et al. Volatiles of bacteria associated with parasitoid habitats elicit distinct olfactory responses in an aphid parasitoid and its hyperparasitoid. Funct Ecol. 2020;34:507-520. https ://doi.

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Characterization of culturable gut bacterial isolates from wild population of melon fruit fly (Bactrocera cucurbitae) and assessing their attractancy potential for sustainable pest management

Cited by 8 publications

References 38 publications

Comparative Microbiomics of Tephritid Frugivorous Pests (Diptera: Tephritidae) From the Field: A Tale of High Variability Across and Within Species

Comparative Microbiomics of Tephritid Frugivorous Pests (Diptera: Tephritidae) From the Field: A Tale of High Variability Across and Within Species

New Insights on the Zeugodacus cucurbitae (Coquillett) Bacteriome

Volatiles of bacteria associated with parasitoid habitats elicit distinct olfactory responses in an aphid parasitoid and its hyperparasitoid

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