Characterization of cryoinjury-induced infarction with manganese-and gadolinium-enhanced MRI and optical spectroscopy in pig hearts

Yang, Yanmin; Sun, Jiankang; Gervai, Patricia Dreessen de; Gruwel, Marco L.H.; Jilkina, Olga; Gussakovsky, Eugene; Yang, Xi; Kupriyanov, V.V.

doi:10.1016/j.mri.2010.02.001

Cited by 14 publications

(21 citation statements)

References 55 publications

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“…Similarly, adult mammals deploy a fibrotic and hypertrophic response following cryoinjury (Yang et al, 2010(Yang et al, , 2011. By contrast, the neonatal heart is characterized by an absence of fibrosis and hypertrophy following cardiac injury (Porrello et al, 2011(Porrello et al, , 2013.…”

Section: Lack Of Fibrotic or Hypertrophic Responses Following Cryoinjmentioning

confidence: 99%

Development of a human cardiac organoid injury model reveals innate regenerative potential

et al. 2017

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The adult human heart possesses a limited regenerative potential following an ischemic event, and undergoes a number of pathological changes in response to injury. While cardiac regeneration has been documented in zebrafish and neonatal mouse hearts, it is currently unknown whether the immature human heart is capable of undergoing complete regeneration. Combined progress in pluripotent stem cell differentiation and tissue engineering has facilitated the development of human cardiac organoids (hCO), which resemble fetal heart tissue and can be used to address this important knowledge gap. This study aimed to characterise the regenerative capacity of immature human heart tissue in response to injury. Following cryoinjury with a dry ice probe, hCO exhibited an endogenous regenerative response with full functional recovery by two weeks following acute injury. Cardiac functional recovery occurred in the absence of pathological fibrosis or cardiomyocyte hypertrophy. Consistent with regenerative organisms and neonatal human hearts, there was a high basal level of cardiomyocyte proliferation, which may be responsible for the regenerative capacity of the hCO. This study suggests that immature human heart tissue has an intrinsic capacity to regenerate.

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Section: Lack Of Fibrotic or Hypertrophic Responses Following Cryoinjmentioning

confidence: 99%

Development of a human cardiac organoid injury model reveals innate regenerative potential

et al. 2017

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“…For details of a pig heart surgery and cryoinjury, see Ref. [30]. The isolated pig hearts were excised and perfused in isovolumic (Langendorff) mode with a Krebs-Henseleit buffer (KHB: 25 mM NaHCO 3 , 118 mM NaCl, 4.7 mM KCl, 1.75 mM CaCl 2 , 1.2 mM MgSO 4 , 0.5 mM ethylenediaminetetraacetic acid [EDTA], and 11 mM glucose) and 6 g/L bovine serum albumin as described elsewhere [30].…”

Section: Pig Heartsmentioning

confidence: 99%

“…[30]. The isolated pig hearts were excised and perfused in isovolumic (Langendorff) mode with a Krebs-Henseleit buffer (KHB: 25 mM NaHCO 3 , 118 mM NaCl, 4.7 mM KCl, 1.75 mM CaCl 2 , 1.2 mM MgSO 4 , 0.5 mM ethylenediaminetetraacetic acid [EDTA], and 11 mM glucose) and 6 g/L bovine serum albumin as described elsewhere [30]. The perfusate was aerated with 95% O 2 /5% CO 2 in a membrane oxygenator providing arterial pO2 of 400 to 550 mm Hg and pH 7.35 to 7.45 at 37 °C.…”

Section: Pig Heartsmentioning

confidence: 99%

“…See Ref. [30] for details. The slice imaging was done within 20 to 25 min after termination of perfusion.…”

Section: Pig Heartsmentioning

confidence: 99%

“…A large area of low OSP (10-30%) in the bottom part of the map relates to a cryoinjured part of the left ventricle. We did not consider it because our previous data [30] suggested little Mb in such scar tissue.…”

Section: Mapping the Isolated Perfused Heartmentioning

confidence: 99%

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Mapping the myoglobin concentration, oxygenation, and optical pathlength in heart ex vivo using near-infrared imaging

Gussakovsky

Yang

Rendell

et al. 2010

Analytical Biochemistry

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Dynamic manganese‐enhanced magnetic resonance imaging can detect chronic cryoinjury‐induced infarction in pig hearts in vivo

Yang

Gervai

Sun

et al. 2011

Contrast Media & Molecular

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The purpose was to investigate whether MnCl2 can serve as an MRI contrast agent to detect chronic cryoinjury infarction in pigs in vivo and whether MnCl2 causes significant hemodynamic disturbances. Hearts were subjected to a topical 2 min cryothermia to establish myocardial infarction (MI). Thereafter GdDTPA‐enhanced MRI was performed at 0, 1, 2 and 3 weeks using a 3 T scanner. Four weeks post‐cryoinjury the pigs underwent in vivo Mn‐enhanced magnetic resonance imaging (MEMRI). MnCl2 (70 μmol/kg, 14 min) was infused i.v. intermittently (n = 4) or continuously (n = 5) and T1‐weighted images were acquired every 2 min simultaneously recording heart rate and arterial blood pressure. Either infusion scheme led to an immediate increment in MR signal intensity (SI) within the left ventricular (LV) blood pool and LV normal and cryoinjured myocardium, which reached a maximum at the end of infusion. No significant difference was observed between the normal and cryoinjured myocardium. After infusion termination, SI decreased faster within the LV blood pool and the MI, as compared with the normal myocardium in either group, resulting in significant contrast between the MI and normal tissue (intermittent: 18 ± 7 vs 49 ± 13%, p = 0.002; continuous: 19 ± 8 vs 36 ± 9%, p = 0.004). Infarction sizes were similar in Mn2+‐ and GdDTPA‐enhanced images at 4 and 3 weeks post injury, respectively. Thus, in vivo MEMRI differentiated infarcted from normal myocardium in pig hearts subjected to 4‐week cryoinjury. Compared with intermittent infusion, continuous infusion minimized hemodynamic fluctuations. Copyright © 2011 National Research Council Canada.

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Characterization of cryoinjury-induced infarction with manganese-and gadolinium-enhanced MRI and optical spectroscopy in pig hearts

Cited by 14 publications

References 55 publications

Development of a human cardiac organoid injury model reveals innate regenerative potential

Development of a human cardiac organoid injury model reveals innate regenerative potential

Mapping the myoglobin concentration, oxygenation, and optical pathlength in heart ex vivo using near-infrared imaging

Dynamic manganese‐enhanced magnetic resonance imaging can detect chronic cryoinjury‐induced infarction in pig hearts in vivo

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