Characterization of Crown Rot Disease of Banana Fruit and Eco-Friendly Quality Improvement Approach during Storage

Jahan, Md. Saroar; Lia, Rizwoana Sharmin; Chowdhury, Md. Estiak Khan; Hasan, Md. Faruk; Islam, Md. Asadul; Sikdar, Biswanath; Khalekuzzaman, Md.

doi:10.9734/mrji/2019/v27i330099

Cited by 10 publications

(6 citation statements)

References 16 publications

(18 reference statements)

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“…The PCR products showed 650 bp length size which was similar to the first isolated pathogen PCR results. Jahan et al [42] reported similar results which support our [43,44,45]. Ehiobu and Ogu, [46] revealed that Manihot esculenta leaf extract was significant at 75 g/L, with 68.2% inhibition against B. theobromae fungus and leaf extracts of Discoria rotundata, and Colocasia exculenta also possess significant in vitro antifungal activities against B. theobromae at the same concentration.…”

Section: Discussionsupporting

confidence: 82%

PCR and Sequencing Base Detection of Gummosis Disease on Citrus aurantifolia Caused by Lasiodiplodia theobromae and Evaluation of Its Antagonisms

Hasan

Islam

Sikdar

2020

JAMB

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Diplodia gummosis disease caused by Lasiodiplodia theobromae is an economically important postharvest fruit decay that occurs on all types of citrus grown in Bangladesh. The present investigation was conducted to isolate and identify the pathogenic fungus responsible for postharvest diplodia gummosis disease (DGD) of citrus as well as the evaluation of its biological control through microbial antagonists. DGD causing pathogen was identified by physiological, morphological and molecular methods. The pathogenic fungus was isolated from the surface of postharvest lime fruits. The optimum growth of the fungi was observed in maltose, 2% NaCl, 15% sugar and pH 7 containing PDA medium at 35⁰C. PCR products of the internal transcribed spacer (ITS) region of the fungus showed approximately 650 bp size clear band in gel electrophoresis. The amplified region of the fungus showed 99.62% similarities with the sequences of L. theobromae. Artificially inoculation of the fungus in malta, musumbi, sweet orange, lime, and guava fruits showed similar size clear band and typical diplodia gummosis disease symptom. Methanol extracts of Datura metal displayed the highest inhibition (75.25%) against the isolated fungus. Non-pathogenic fungi Trichoderma viride showed the highest antagonistic efficiency followed by Neofusicoccum mangifera against the isolated fungus. The tested soil bacteria did not show significant antagonistic activity against the isolated fungus. Therefore, the DGD of citrus control system should be integrated into the overall citrus postharvest decay control system to reduce all citrus postharvest diseases and to protect fresh citrus fruit values.

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Section: Discussionsupporting

confidence: 82%

PCR and Sequencing Base Detection of Gummosis Disease on Citrus aurantifolia Caused by Lasiodiplodia theobromae and Evaluation of Its Antagonisms

Hasan

Islam

Sikdar

2020

JAMB

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“…The PCR products showed clear cut band of 650bp length size which is 100% similar to our first isolated fungus. Jahan et al [19] reported similar results for crown rot disease of banana fruit causing fungus which support our present findings. The above results confirmed the isolated fungus as Pichia kudriavzevii which is responsible for brown rot disease of Citrus sinensis fruit.…”

Section: Discussionsupporting

confidence: 92%

“…Choi et al [18] showed similar morphological and microscopic results for a fungal isolate from nuruk and the pathogen was identified as P. kudriavzevii. Jahan et al [19] observed cylindrical, septate and slightly rounded ends conidia under light microscopic evaluation in case of crown rot disease of banana which supports our present findings. In the growth profiling of the present fungal isolate, PDA medium was found to be best for growth and development.…”

Section: Discussionsupporting

confidence: 91%

“…P. kudriavzevii showed strong thermotolerance [21]. On the contrast, Oberoi et al, [22] and Greppi et al [23] reported as high as 45°C and Choi et al [19] at 44°C for best growth and development of P. kudriavzevii. Hasan et al [20] reported the best response for growth and development of a fungal stain in PDA medium A C D B having 1.41gm/L fructose which supports our present investigation.…”

Section: Discussionmentioning

confidence: 95%

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Characterization and Antagonistic Control Measures of Pichia kudriavzevii Yeast like Fungus in Brown Rot Disease of Citrus sinensis (L.) Osbeck

Hasan¹,

Islam²,

Sikdar³

2019

IJAM

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Citrus sinensis is one of the most important Vitamin C containing fruit all over the world which is badly infected by brown rot disease (BRD). The present research work was designed to isolate and characterize the pathogenic fungus responsible for BRD of C. sinensis as well as evaluation of its antagonistic control measures. The causal agent of BRD was isolated and characterized using physiological, morphological and molecular methods. The pathogenic fungus was isolated from the skin of sweet orange. The isolated fungus was grown in different nutrients media, temperature, pH, sugar concentrations and different carbohydrates. The highest growth of the fungi was observed in 5% fructose containing PD broth media at 37 0 C, pH 7.5 and 0.05gm/L NaCl concentration. PCR amplification of the isolated fungal DNA by internal transcribed spacer (ITS) region products showed approximately 650bp size of clear cut band. sequencing analysis showed that the amplified its region of the fungus (GenBank Submission# SUB5672073) has 99.80% similarities with the sequences of Pichia kudriavzevii isolate 11-577 its-1 partial sequence. In pathogenicity assay, artificially infected P. kudriavzevii fungus in lemon, malta and apple fruits showed a typical brown rot disease symptoms and the isolates showed similar size of 650bp clear band for PCR products. In antifungal activity assay, the methanol extracts of Allium sativum displayed highest 17.18±0.66 mm diameter zone of inhibition against the isolated fungus. Rhizobium leguminosarum and Alternaria spp. showed significant antagonistic effect in coculture with the isolated fungus. The present research work would be helpful to control the devastating brown rot disease of Citrus sinensis.

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“…Some essential oils and plant extracts have been found to have a similar antifungal activity to commercial fungicides used against crown rot disease. Jahan et al (2019) reported that the methanol extract of garlic, A. sativum, has similar fungicidal activity as chemical fungicides like carbendazim and kanamycin B against crown rot. Spraying emulsions of basil oil on bananas was observed to control anthracnose and crown rot in bananas stored for 21 days.…”

Section: Bananamentioning

confidence: 99%

Essential oils and plant extracts for tropical fruits protection: From farm to table

Israfi¹,

Ali²,

Manickam³

et al. 2022

Front. Plant Sci.

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The tropical fruit industry in Malaysia makes up a large proportion of the agriculture sector, contributing to the local economy. Due to their high sugar and water content, tropical fruits are prone to pathogenic infections, providing optimal microorganism growth conditions. As one of the largest exporters of these fruits globally, following other Southeast Asian countries such as Thailand, Indonesia and the Philippines, the quality control of exported goods is of great interest to farmers and entrepreneurs. Traditional methods of managing diseases in fruits depend on chemical pesticides, which have attracted much negative perception due to their questionable safety. Therefore, the use of natural products as organic pesticides has been considered a generally safer alternative. The extracts of aromatic plants, known as essential oils or plant extracts, have garnered much interest, especially in Asian regions, due to their historical use in traditional medicine. In addition, the presence of antimicrobial compounds further advocates the assessment of these extracts for use in crop disease prevention and control. Herein, we reviewed the current developments and understanding of the use of essential oils and plant extracts in crop disease management, mainly focusing on tropical fruits. Studies reviewed suggest that essential oils and plant extracts can be effective at preventing fungal and bacterial infections, as well as controlling crop disease progression at the pre and postharvest stages of the tropical fruit supply chain. Positive results from edible coatings and as juice preservatives formulated with essential oils and plant extracts also point towards the potential for commercial use in the industry as more chemically safe and environmentally friendly biopesticides.

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Characterization of Crown Rot Disease of Banana Fruit and Eco-Friendly Quality Improvement Approach during Storage

Cited by 10 publications

References 16 publications

PCR and Sequencing Base Detection of Gummosis Disease on Citrus aurantifolia Caused by Lasiodiplodia theobromae and Evaluation of Its Antagonisms

PCR and Sequencing Base Detection of Gummosis Disease on Citrus aurantifolia Caused by Lasiodiplodia theobromae and Evaluation of Its Antagonisms

Characterization and Antagonistic Control Measures of Pichia kudriavzevii Yeast like Fungus in Brown Rot Disease of Citrus sinensis (L.) Osbeck

Essential oils and plant extracts for tropical fruits protection: From farm to table

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