Characterization of conditional lethal mutants of bacteriophage P2

Lindahl, Gunnar

doi:10.1007/bf00267114

Cited by 33 publications

(15 citation statements)

References 24 publications

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“…H and G are essential genes located in the distal portion of the P2 VWJIHG transcription unit (56)(57)(58)88). This transcription unit is separated from the F,F,JETUD transcription unit, which also contains tail (36,96).…”

Section: Resultsmentioning

confidence: 99%

“…5E) and from 41 to 84% identity for region T (about 154 residues in length). [93], P2 H [66] K3 37 [100], Ml 37 [45], Ox2 37 [45] K3 38 [63], Ml 38 [50] [62], K3 37 [54], T2 37 [53] TuIb 37 [91], Tula 37 [85], X Stf [76], e14 ORF Pmin [36] Tula 37 [47], TuIb 37 [47], X Stf [41] T3 Tf [87] T3 Tf [90], K3 37 [29] T3 Tf [39] T2 37 [61], T4 37 [60] K3 37 [69], T2 37 [68], T4 37 [62] TuIb 37 [88], T4 37 [85], X Stf [72], e14 ORF Pmin [36] TuIb 37 [84], X Stf [58], T4 37 [47] T4 37 [91], Tula 37 [88], X Stf [73], e14 ORF Pmin [36] Tula 37 ...…”

Section: Resultsmentioning

confidence: 99%

“…To date, 12 tail genes and six tail proteins of P2 have been identified (55,57,58,88,90). Tail genes R and S are located in the distal portion of the ONMLKRS transcription unit (Fig.…”

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confidence: 99%

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DNA sequences of the tail fiber genes of bacteriophage P2: evidence for horizontal transfer of tail fiber genes among unrelated bacteriophages

1992

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We have determined the DNA sequence of the bacteriophage P2 tail genes G and H, which code for polypeptides of 175 and 669 residues, respectively. Gene H probably codes for the distal part of the P2 tail fiber, since the deduced sequence of its product contains regions similar to tail fiber proteins from phages Mu, P1, lambda, K3, and T2. The similarities of the carboxy-terminal portions of the P2, Mu, ann P1 tail fiber proteins may explain the observation that these phages in general have the same host range. The P2 H gene product is similar to the products of both lambda open reading frame (ORF) 401 (stf, side tail fiber) and its downstream ORF, ORF 314. If 1 bp is inserted near the end of ORF 401, this reading frame becomes fused with ORF 314, creating an ORF that may represent the complete stf gene that encodes a 774-amino-acid-long side tail fiber protein. Thus, a frameshift mutation seems to be present in the common laboratory strain of lambda. Gene G of P2 probably codes for a protein required for assembly of the tail fibers of the virion. The entire G gene product is very similar to the products of genes U and U' of phage Mu; a region of these proteins is also found in the tail fiber assembly proteins of phages TuIa, TuIb, T4, and lambda. The similarities in the tail fiber genes of phages of different families provide evidence that illegitimate recombination occurs at previously unappreciated levels and that phages are taking advantage of the gene pool available to them to alter their host ranges under selective pressures.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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DNA sequences of the tail fiber genes of bacteriophage P2: evidence for horizontal transfer of tail fiber genes among unrelated bacteriophages

1992

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“…1). We therefore performed in vitro phage reconstitution experiments (30,49) to determine whether this gene encodes another essential head function, since its product does not play a role in lysis (Fig. 5).…”

Section: Resultsmentioning

confidence: 99%

“…In spite of the early isolation of lysis-deficient P2 mutants (28,29,49), the specific lysis functions have not been defined; this prompted the study reported here. P2 Kts6O was isolated by a procedure designed to find temperature-sensitive lysozyme mutants, by looking for plaques which cannot form halos when exposed to chloroform at the nonpermissive temperature (30). This mutant had the expected phenotype for lysis deficiency: it was affected in a P2 late function and failed to lyse infected cells but did stop them from growing.…”

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confidence: 99%