Functions involved in bacteriophage P2-induced host cell lysis and identification of a new tail gene

Ziermann, Rainer; Bartlett, Becky A.; Calendar, Richard; Christie, Gail E.

doi:10.1128/jb.176.16.4974-4984.1994

Cited by 47 publications

(49 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…43,44 Amber mutants in lysB exhibit delayed lysis in non-permissive cells. 43 The lysC gene, which overlaps the lysB gene, was originally identified by a mutation that overcomes the P2 growth defect conferred by a temperature-sensitive mutation in the b' subunit of RNA polymerase. 45 LysB and LysC are functional homologues of lambda Rz and Rz1, which make up the spanin complex that fuses the inner and outer membranes to complete host cell lysis.…”

Section: The Lysis Genesmentioning

confidence: 99%

“…Genes X, R and S are at the end of the operon that begins with gene O, and flank the lysis module. Gene X encodes a tail function, according to in vitro complementation studies, 43 and gpX has recently been localized to the top of the baseplate. 47 Nonsense mutants in gene R make abnormally long tails lacking the head-tail connector, and giant naked tail tubes.…”

Section: The Lysis Genesmentioning

confidence: 99%

“…Gene Y encodes a holin, which allows the P2 lysozyme, the product of gene K, to access the peptidoglycan. 43,44 The lysA gene product functions as an antiholin, needed to delay the action of gpY until the optimal lysis time. 43,44 Amber mutants in lysB exhibit delayed lysis in non-permissive cells.…”

Section: The Lysis Genesmentioning

confidence: 99%

“…43,44 The lysA gene product functions as an antiholin, needed to delay the action of gpY until the optimal lysis time. 43,44 Amber mutants in lysB exhibit delayed lysis in non-permissive cells. 43 The lysC gene, which overlaps the lysB gene, was originally identified by a mutation that overcomes the P2 growth defect conferred by a temperature-sensitive mutation in the b' subunit of RNA polymerase.…”

Section: The Lysis Genesmentioning

confidence: 99%

See 3 more Smart Citations

Bacteriophage P2

Christie

Calendar

2016

Bacteriophage

View full text Add to dashboard Cite

show abstract

Section: The Lysis Genesmentioning

confidence: 99%

Section: The Lysis Genesmentioning

confidence: 99%

Section: The Lysis Genesmentioning

confidence: 99%

Section: The Lysis Genesmentioning

confidence: 99%

See 2 more Smart Citations

Bacteriophage P2

Christie

Calendar

2016

Bacteriophage

View full text Add to dashboard Cite

show abstract

“…Regulation of the time point of lysis has been reported to occur also during bacteriophage P2 lytic infection, in which two nonessential genes, lysA and lysB, are involved (37). However, their gene products have only a minor effect, resulting in a 2-to 5-min acceleration or delay of lysis when lysA or lysB, respectively, is deleted.…”

Section: Discussionmentioning

confidence: 99%

Three functions of bacteriophage P1 involved in cell lysis

1996

View full text Add to dashboard Cite

Amber and deletion mutants were used to assign functions in cell lysis to three late genes of bacteriophage P1. Two of these genes, lydA and lydB of the dar operon, are 330 and 444 bp in length, respectively, with the stop codon of lydA overlapping the start codon of lydB. The third, gene 17, is 558 bp in length and is located in an otherwise uncharacterized operon. A search with the predicted amino acid sequence of LydA for secondary motifs revealed a holin protein-like structure. Comparison of the deduced amino acid sequence of gene 17 with sequences of proteins in the SwissProt database revealed homologies with the proteins of the T4 lysozyme family. The sequence of lydB is novel and exhibited no known extended homology. To study the effect of gp17, LydA, and LydB in vivo, their genes were cloned in a single operon under the control of the inducible T7 promoter, resulting in plasmid pAW1440. A second plasmid, pAW1442, is identical to pAW1440 but has lydB deleted. Induction of the T7 promoter resulted in a rapid lysis of cells harboring pAW1442. In contrast, cells harboring pAW1440 revealed only a small decrease in optical density at 600 nm compared with cells harboring vector alone. The rapid lysis phenotype in the absence of active LydB suggests that this novel protein might be an antagonist of the holin LydA.

show abstract

Bacteriophages ofPseudomonas

and

Lavigne

2008

Pseudomonas

View full text Add to dashboard Cite

Functions involved in bacteriophage P2-induced host cell lysis and identification of a new tail gene

Cited by 47 publications

References 48 publications

Bacteriophage P2

Bacteriophage P2

Three functions of bacteriophage P1 involved in cell lysis

Bacteriophages ofPseudomonas

Contact Info

Product

Resources

About