Characterization of collagen in non-small cell lung carcinoma with second harmonic polarization microscopy

Abstract

Polarization second harmonic microscopy was used for collagen imaging in human non-small cell lung carcinoma and normal lung tissues ex vivo and revealed significant differences in the nonlinear susceptibility component ratio, demonstrating potential use in cancer diagnosis.

Ultrastructural features of collagen in thyroid carcinoma tissue observed by polarization second harmonic generation microscopy

“…Changes in χ (2) ZZZ '/χ (2) ZXX ' for collagen have previously been ascribed to possible alterations in the helical angle of the triple helix and/or the arrangement of the triple helices into fibrils and fibers and/or the distribution of fibrils and fibers within the laser focal volume [7,17]. Increase in χ (2) ZZZ '/χ (2) ZXX ' is attributed to increased distribution of fibers and fibrils in the focal volume, which can be understood from Eq.…”

“…In principle then, cancer diagnosis can be improved by developing a technique that identifies and quantifies structural details in the ECM. Since collagen is the most abundant protein in the ECM and is known to have strong second harmonic generation (SHG) signals without staining, its content and spatial micro-distribution can be visualized by SHG microscopy [7]. Further, by applying polarization-sensitive measurements, the second-order nonlinear optical susceptibility tensor component ratio, χ (2) ZZZ '/χ (2) ZXX ', and the degree of linear polarization, DOLP, can be measured.…”

“…These metrics provide additional information on the collagen ultrastructural characteristics that is complementary to the concentration and spatial distribution measures. Polarization-sensitive SHG microscopy has been previously applied to determine differences in normal and malignant breast tissue [8], models of ovarian cancer [9], and non-small cell lung carcinoma [7]. In this study, we use polarization-in, polarization-out (PIPO) SHG microscopy to investigate classical papillary thyroid carcinoma (cPTC) and follicular variant papillary thyroid carcinoma (FVPTC).…”

Second harmonic generation double stokes Mueller polarimetric microscopy of myofilaments

“…By retrieving R and δ distribution maps in a sample under investigation, variations in the molecular ultrastructure can be studied. For example, changes in collagen R ratio have been observed between healthy and tumor tissue [6]. In this paper, R and δ are obtained by a global fitting routine based on the Levenberg-Marquardt (LM) algorithm with R, δ and amplitude as free parameters to minimize the total root-mean-square error (RMSE) between the measured laboratory-frame χ (2) components and components obtained by Eq.…”

“…The first SHG polarimetric analysis in biological tissue was demonstrated in rat-tail tendon by Freund, Deutsch and Sprecher [2]. Second-order susceptibility tensor χ (2) component ratios have been measured in biological samples such as collagen fibers [3][4][5][6], myofibrils [7][8][9][10] and starch granules [11], as well as crystalline nanostructures [12,13]. Jones formalism has been used for polarimetric analysis of SHG microscopy images [14,15].…”