Characterization of clarithromycin resistance in isolates of Helicobacter pylori from the UAE

Alfaresi, Mubarak; Elkoush, Abida A.

doi:10.1007/s12664-010-0034-z

Cited by 12 publications

(9 citation statements)

References 23 publications

(25 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Subgroup analysis based on PCR methods Several PCR-based methods were applied in these 22 studies. Including FRET-MCA-PCR, 22,25,27,54,[56][57][58] PCR-based sequencing, 52,59,60 TaqMan PCR, 61-64 FISH, 32,33,52,59 allele-specific-PCR, 20,47,65 and dual priming oligonucleotide-PCR, 58 GenoType HelicoDR, 51 PCR-DHPLC, 23 and RFLP-PCR. 31 The results showed that the sensitivity and specificity…”

Section: Performance Of Genotypic Methods In Gastric Biopsy Samplesmentioning

confidence: 99%

A systematic review and meta‐analysis of genotypic methods for detecting antibiotic resistance in Helicobacter pylori

Wang

et al. 2018

Helicobacter

View full text Add to dashboard Cite

show abstract

Section: Performance Of Genotypic Methods In Gastric Biopsy Samplesmentioning

confidence: 99%

A systematic review and meta‐analysis of genotypic methods for detecting antibiotic resistance in Helicobacter pylori

Wang

et al. 2018

Helicobacter

View full text Add to dashboard Cite

show abstract

“…Unfortunately, resistance to this antibiotic is increasing worldwide and failure treatment is attributed to acquired antibiotic resistance of the organism or lack of compliance with the antibiotic regimen ( 7 ). The prevalence of H. pylori resistance to clarithromycin varies among different countries; 12% in Japan, 1.7% - 23.4% in Europe and 10.6% - 25% in north America ( 5 , 6 , 8 ). Antimicrobial activity of clarithromycin is attributed to inhibition of protein synthesis via attaching to bacterial ribosomal 50S subunit ( 9 ).…”

Section: Introductionmentioning

confidence: 99%

Detection of 23SrRNA Mutations Strongly Related to Clarithromycin Resistance in Helicobacter pylori Strains Isolated From Patients in the North of Iran

Eghbali

Mojtahedi

Ansar

et al. 2016

Jundishapur J Microbiol

View full text Add to dashboard Cite

Background:Helicobacter pylori is curved Gram negative and microaerophilic bacilli that have infected half of the world’s population. It is recognized as the causative agent of duodenal ulcer, gastritis peptic ulcer, mucosa-associated lymphoid tissue (MALT) lymphoma and is associated with gastric adenocarcinoma. Resistance to clarithromycin is related to point mutations in 23SrRNA gene on nt 2143 and 2144, when A turns to G, and A2143G is the most important type. These mutations lead to reduced affinity of antibiotics to their ribosomal target and are considered as the main cause of treatment failure.Objectives:The aim of this study was to determine the frequency of A2143G point mutation in 23SrRNA of H. pylori strains isolated from gastric biopsies of patients in Rasht, north of Iran, by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).Patients and Methods:A descriptive study was performed on 89 H. pylori strains, which were isolated from gastric biopsies of patients with gastric disorders such as gastritis, peptic ulcer, duodenal ulcer, non-ulcer dyspepsia and gastric adenocarcinoma. Isolated strains were tested for clarithromycin resistance using as breakpoint a minimum inhibitory concentration (MIC) of ≥ 1 mg/L by the E-test. The presence of H. pylori DNA was confirmed by amplifying the ureC (glmM) gene by PCR. Also, point mutation on 23SrRNA gene (A2142G and A2143G) was detected by PCR-RFLP using MboII and BsaI restriction endonucleases in all extracted DNA.Results:Of the 89 H. pylori isolates, eighty-four were susceptible to clarithromycin, while five (5.6%) were resistant. All DNA samples of resistant strains, which were treated with BsaI had A2143G mutation. There was no point mutation in the sensitive strains of H. pylori. Also, we detected no mutation on nt A2142G of resistant strains.Conclusions:In the present study, the frequency of clarithromycin resistance was lower than the other studies conducted in Iran. Resistance frequency in samples isolated from gastric ulcer was higher than other gastric disorders. Women and patients aged more than 60 years old showed the most resistance frequency in this study. All resistant strains had the A2143G genotype.

show abstract

“…The resistance to clarithromycin in H. pylori has been shown to be due to point mutations in the peptidyltransferase region of domain V of the 23S rRNA. The most common mutation is an A-to-G transition at position 2143 (A2143G), but three point mutations -A2142G, A2144G and T2182C -have also been described (Taylor et al, 1997;Alfaresi & Elkoush, 2010). The presence of the A2143G point of mutation has been shown to significantly affect the eradication rate of H. pylori.…”

Section: Introductionmentioning

confidence: 99%

Resistance to clarithromycin and genotypes in Helicobacter pylori strains isolated in Sicily

Fasciana

Calà

Bonura

et al. 2015

Journal of Medical Microbiology

View full text Add to dashboard Cite

The resistance of Helicobacter pylori strains to clarithromycin is increasing in several developed countries and their association with a genetic pattern circulation has been variously explained as related to different geographical areas. In this study we have reported: the prevalence of the resistance of H. pylori, isolated in Sicily, to clarithromycin; the principal point of mutation associated with this resistance; and the more frequent association between resistance to clarithromycin and cagA, the EPIYA motif, and the vacA and oipA genes. Resistance to clarithromycin was detected in 25 % of cases, the main genetic mutation involved being A2143G. The cagA gene was present in 48 % of cases and the distribution of the EPIYA motif was: ABC in 35 cases; ABCC in 8 cases; ABCCC in 2 cases; ABC-ABCC in 2 cases; and ABC-ABCC-ABCCC in 1 case. Regarding the vacA allele, an s1i1m1 combination was detected in 35 % of cases, s1i1m2 in 12 %, s1i2m2 in 12 %, s2i2m2 in 40 %, and a double s1m1-m2 mosaic in 1 % of cases. The status of the oipA gene was 'off' in 45 % of cases and 'on' in 55 %. Resistance to clarithromycin was found to be high in Sicily, but no correlation was found among resistance to clarithromycin, the vacA gene and oipA status; a higher correlation was observed between resistant strains and cagA-negative strains.

show abstract

Characterization of clarithromycin resistance in isolates of Helicobacter pylori from the UAE

Abstract: Clarithromycin resistance was common in this small collection of H. pylori isolates from the UAE. The A2142G and A2143G mutations were associated with clarithromycin resistance.

Cited by 12 publications

References 23 publications

A systematic review and meta‐analysis of genotypic methods for detecting antibiotic resistance in Helicobacter pylori

A systematic review and meta‐analysis of genotypic methods for detecting antibiotic resistance in Helicobacter pylori

Detection of 23SrRNA Mutations Strongly Related to Clarithromycin Resistance in Helicobacter pylori Strains Isolated From Patients in the North of Iran

Resistance to clarithromycin and genotypes in Helicobacter pylori strains isolated in Sicily

Contact Info

Product

Resources

About