Characterization of chromosome composition of sugarcane in nobilization by using genomic in situ hybridization

Abstract

BackgroundInterspecific hybridization is an effective strategy for germplasm innovation in sugarcane. Nobilization refers to the breeding theory of development and utilization of wild germplasm. Saccharum spontaneum is the main donor of resistance and adaptive genes in the nobilization breeding process. Chromosome transfer in sugarcane is complicated; thus, research of different inheritance patterns can provide guidance for optimal sugarcane breeding.ResultsThrough chromosome counting and genomic in situ hybridization, we found that six clones with 80 chromosomes were typical S. officinarum and four other clones with more than 80 chromosomes were interspecific hybrids between S. officinarum and S. spontaneum. These data support the classical view that S. officinarum is characterized by 2n = 80. In addition, genomic in situ hybridization showed that five F1 clones were products of a 2n + n transmission and one F1 clone was the product of an n + n transmission in clear pedigree noble hybrids between S. officinarum and S. spontaneum. Interestingly, Yacheng 75–408 and Yacheng 75–409 were the sibling lines of the F1 progeny from the same parents but with different genetic transmissions.ConclusionsThis is the first clear evidence of Loethers, Crystallina, Luohanzhe, Vietnam Niuzhe, and Nanjian Guozhe were typical S. officinarum by GISH. Furthermore, for the first time, we identified the chromosome transmission of six F1 hybrids between S. officinarum and S. spontaneum. These findings may provide a theoretical basis for germplasm innovation in sugarcane breeding and guidance for further sugarcane nobilization.Electronic supplementary materialThe online version of this article (10.1186/s13039-018-0387-z) contains supplementary material, which is available to authorized users.

Cytogenetic and Molecular Approaches to Detect Alien Chromosome Introgression and Its Impact in Three Successive Generations of <i>Erianthus procerus</i> × <i>Saccharum</i>

“…An atypical S. officinarum would have been originated by natural hybridization with related species at its place of origin. Hence there is a chance for S. spontaneum genome in it and its presence has been proved through molecular cytogenetic tools (Piperidis et al 2010a, Yu et al 2018. This genome might have induced the 2n chromosome transmission from the other parent (E. procerus).…”

The Effect of Colchicine onGenome Size and Agronomical Traits and Correlation with Sugarcane Putative Mutants Production

“…Tebu (Saccharum officinarum L.) merupakan tanaman poliploid yang memiliki delapan set kromosom dalam selnya dan disebut oktaploid (Comai 2005). Tebu yang dibudidayakan adalah hasil persilangan interspesifik antara S. officinarum dan S. spontaneum yang memiliki lebih dari 80 kromosom (Yu et al 2018). Pemuliaan tebu telah banyak dilakukan baik secara konvensional maupun inkonvensional.…”

Selection and breeding programs for disease resistance in sugarcane

“…This process of 2n + n transmission is repeated once again when the hybrid is backcrossed to S.officinarum as the female [19]. 2n gametes may or may not function in further backcrosses depending on the specific S.officinarum clones that may be used [20]. It is critical to note that while uneven chromosome transmission is the norm in S.officinarum x S.spontaneum hybrids as opposed to the exception, from these interspecific crosses rare times of n+n progeny were pronounced, regularly involving S.spontaneum [21].…”