Characterization of Chlorella sorokiniana growth properties in monosaccharide-supplemented batch culture

Abstract

To reveal growth properties of Chlorella sorokiniana UTEX 1230, four monosaccharides (glucose, fructose, galactose and xylose) were individually supplemented into medium as carbon sources for the cultivation of C. sorokiniana UTEX 1230. Supplementation with glucose increased OD750, biomass and lipid yield but decreased protein abundance per unit dry weight of biomass under all concentrations examined, the maximum OD750, biomass and lipid yield increased 2.04, 6.78 and 12.43 times, respectively, compared with autotrophic controls. A low concentration of glucose (<4 g/L) simultaneously promoted the biosynthesis of chlorophylls and protein abundance per unit culture volume, but decreased the lipid content per unit dry weight of biomass and all supplemented glucose can be exhausted within 7 days. Higher glucose concentrations (≥4 g/L) decreased the biosynthesis of chlorophylls and protein abundance per unit culture volume, but increased the lipid content per unit dry weight of biomass. In glucose supplemented scenario, C. sorokiniana UTEX 1230 growth was light-independent. Supplementation with fructose promoted C. sorokiniana UTEX 1230 growth to a much lesser extent compared with glucose, whereas supplementation with galactose had no effect and supplementation with xylose even inhibited growth. Our findings represent basic experimental data on the effect of monosaccharides and can serve as the basis for a robust cultivation system to increase biomass and lipid yield.

Utilization of Nannochloropsis oceanica alga for biodiesel production and the de-lipidated biomass for improving Red tilapia aquaculture

“…Consequently, the growth rate was slightly decreased when fructose was used as the carbon source compared to glucose. Similarly, in our experiment the tested microalga N.oceanica showed high lipid accumulation in the order of glucose, fructose but fewer productions were seen in lactose (Chai et al, 2018;Velu et al, 2015). Otherwise, In the work of Morales-Sanchez et al (2013), fructose promoted growth more than glucose because other species show the opposite response, especially considering Neochloris sp., which preferred glucose and consumption of fructose was null over a period of 5 days .That may be attributed to that each .…”

“…Similar studies using the same carbon sources by Velu et al (2015) revealed that the biomass yield and lipids contents were considerably increased with fructose in relation to controls. Supplementation with lower glucose (0.5-1.0 g/L) significantly promoted the synthesis of chlorophylls and protein availability per unit culture, but decreased the lipid quantity per unit in dry weight bases of biomass under light conditions, whereas the addition of overdose glucose concentrations (4, 8 or 16 g/L) resulted in a light yellow appearance (Chai et al (2018). They also cleared that high concentrations of fructose led to a green-colored culture and that observation agree with our present study when low glucose concentrations of 0.5g/L in the light decreased the biomass and green pigment whereas the same fructose supplementation increased the green appearance and biomass content while lactose addition caused a subtle change in pigment contents.…”

Long-term responses of Scots pine (Pinus sylvestris L.) and European beech (Fagus sylvatica L.) to the contamination of light soils with diesel oil

“…The absorbance spectrum (400 to 800 nm) was measured, and absorbance peaks were determined at 750.0, 648.0, 665.0, 480.0, 435.0, and 415.0 nm with the Hitachi U-1800 spectrophotometer (Tokyo, Japan). The concentrations of chlorophylls a and b , carotenoid content, and the phaeophytization quotient were calculated according to the following formulas (Chai et al 2018 ): where Chla, Chlb chlorophyll concentration in plant material [μg·mg −1 ] V e volume of DMSO extract ( V e = 4 ml) x dilution coefficient m sample weight [mg] where C Chla concentration of chlorophyll a in the extract [μg·ml −1 ] C Chlb concentration of chlorophyll b in the extract [μg·ml −1 ] A i solution absorbance at i th wavelength where TCC total carotenoid content Chla, Chlb chlorophyll content in plant material [μg·mg −1 ] A i solution absorbance at i th wavelength …”

Comparison of the Photoautotrophic Growth Regimens of Chlorella sorokiniana in a Photobioreactor for Enhanced Biomass Productivity

“…The practical application of microalgae metabolic potential can be limited by certain factors, such as photoinhibition and a slow response to different light irradiance, which lead to a low yield of biomass or target products. To increase the cell growth rate, the efficiency of biomass production, and the yield of target products, which means the effectiveness of various microalgae-based technologies, light intensity, photoperiod, temperature, pH, adequacy of macro- and micronutrients, and cultivation regimen (autotrophy, heterotrophy, and mixotrophy) must be thoroughly studied [ 4 , 7 , 8 , 9 ].…”