Utilization of Nannochloropsis oceanica alga for biodiesel production and the de-lipidated biomass for improving Red tilapia aquaculture

Abstract

This work was carried out, firstly for investigating the different effects of various organic carbon sources [fructose (A), lactose (B), glucose (C)], and nitrogen sources [poultry manure extract (PM) and rumen liquor (RL)] which used at ratio 50:50% F/2 medium on maximizing production of biodiesel from the marine microalga Nannochloropsis oceanica. Secondly, we apply the residual lipid-free biomass as feed additives in Red tilapia aquaculture. In phase1, the biomass (DW), biodiesel contents and biochemical compositions of N. oceanica were determined. The results showed varied biomass and biochemical analysis in DW bases between treatments. However, A was achieved significantly the maximum DW for the algal biomass and protein percentage, whereas lipid accumulation was the most in RL as well as achieving the highest biodiesel content. In phase 2, algal biomass by-product (lipid-free) from the superior treatments A and RL were applied at 4 rates (1,2,3 and 4) for concentrations 25,50,75&100%, respectively that substituting soybean for feeding red tilapia larvae as compared with control CO. The initial weight and length of the larvae were 0.32g and 1.9 cm, respectively continued for a duration of 30 days of the experiment. After the end of the experimental period , growth performance, survival percentage (SR%) and biochemical analysis were estimated. The results revealed that SR% was higher significantly for RL2 than A1 (93% & 90%) as compared to control (86.7%). Accordingly, red tilapia carcass attained maximum protein contents for diets A1 & RL2 in whereas lipid content was maximum in control. Finally, authors suggesting the use of Roman liquor as a novel treatment for cost-effective promoting biodiesel content and the remained biomass with 50% substitution of soybean in the diet (RL2)achieved also good results for improving red tilapia aquaculture.

Effect of Various Bicarbonate Supplements on Biodiesel Production and Valuable Biochemical Components of the Marine Eustigmatophyceae Nannochloropsis oculata (Droop)

“…Similarly, White et al (2013) showed that there is no effect of sodium bicarbonate addition on the composition of fatty acids in Tetraselmis suecica cultures, while a marked effect was observed on N. salina cultures. Our data revealed that Palmitic acid (C16:0) was the main saturated fatty acid and this result was in agreement with Abugrara et al (2019), who declared that palmitic acid is the common component in the crude lipids of the N. oceanica. The data explained that SFA was predominantly in N. oculata cultured on B4 medium (100% SB) than MUFA and PUFA recorded at the same medium.…”

“…Suen et al (1987) and Alonso et al (2000) proposed that nitrogen limitation has been developed in order to transfer microalgal metabolism to lipid production. Chisti (2007) and Abugrara et al (2019), have previously investigated the cellular storage lipids of microalgae used in biodiesel processing. Nannochloropsis is a candidate applied for the development of biofuel due to its high level of lipid (Moazami et al, 2012).…”

Biodiesel Production from the Marine Alga Nannochloropsis oceanica Grown on Yeast Wastewater and the Effect on Its Biochemical Composition and Gene Expression

“…Biodiesel can be produced from N. oceanica grown in yeast wastewater due to the high palmitic acid (C16:0) level (29.24% ± 0.11) at Y2 (1.25%) yeast wastewater dilution. According to our results, palmitic acid was the most common SFA in all treatments with its highest level at Y2 (1.25%) yeast wastewater dilution, and this finding supported the findings of Abugrara et al [58] who found palmitic acid to be an essential part in the basic lipids of N. oceanica. According to our results, SFA was more prevalent in N. oceanica than MUFA and PUFA for all treatments, Guihéneuf and Stengel [59] suggested this result.…”

Effects of nitrogen starvation on protein and carbohydrate contents of some marine microalgae and their efficiency as food for the rotifer Brachionus plicatilis (Müller)

“…Microalgae have been used for mass production and enrichment of rotifers due to the content of essential nutrients such as polyunsaturated fatty acids, vitamins, amino acids and pigments that can be transferred to superior trophic levels. Diets such as microalgae, lipid or lipids plus proteins and carbohydrates have been employed (Abugrara et al, 2019). Enrichment of rotifers could be attained by feeding those microalgae rich with those EFA.…”