Characterization of chitinase from Exiguobacterium antarcticum and its bioconversion of crayfish shell into chitin oligosaccharides

Lv, Jiran; Zhang, Yumeng; Ma, Meihu; Oh, Deog‐Hwan; Fu, Xing

doi:10.1016/j.foodres.2022.111517

Cited by 17 publications

(6 citation statements)

References 55 publications

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“…For chitinase from Cellulosimicrobium funkei HY-13, the specific activities of rChiJ for colloidal shrimp shell chitin and colloidal crab shell chitin are 16.0 U/mg and 8.1 U/mg, respectively [65]. However, (GlcNAc) 2 is obtained as the major end product regardless of the used substrates, i.e., shells of shrimp, chitin, and cray fish [52,65]. For the long-chain COSs, transglycosylation (TG) of chitinases has gained much attention for use in agricultural applications.…”

Section: Referencesmentioning

confidence: 99%

Chitinase-Assisted Bioconversion of Chitinous Waste for Development of Value-Added Chito-Oligosaccharides Products

Taokaew

Kriangkrai

2023

Biology

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Chito-oligosaccharides (COSs) are the partially hydrolyzed products of chitin, which is abundant in the shells of crustaceans, the cuticles of insects, and the cell walls of fungi. These oligosaccharides have received immense interest in the last few decades due to their highly promising bioactivities, such as their anti-microbial, anti-tumor, and anti-inflammatory properties. Regarding environmental concerns, COSs are obtained by enzymatic hydrolysis by chitinase under milder conditions compared to the typical chemical degradation. This review provides updated information about research on new chitinase derived from various sources, including bacteria, fungi, plants, and animals, employed for the efficient production of COSs. The route to industrialization of these chitinases and COS products is also described.

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Section: Referencesmentioning

confidence: 99%

Chitinase-Assisted Bioconversion of Chitinous Waste for Development of Value-Added Chito-Oligosaccharides Products

Taokaew

Kriangkrai

2023

Biology

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“…Powdery chitin, xylan, microcrystalline cellulose, and chitosan with a degree of deacetylation (DDA) of 85 to 95% were purchased from Yuanye Biotechnology (Shanghai, China). The colloidal and ball milled-chitin were prepared according to a previously described method [ 51 ].…”

Section: Methodsmentioning

confidence: 99%

Biochemical Properties of a Cold-Active Chitinase from Marine Trichoderma gamsii R1 and Its Application to Preparation of Chitin Oligosaccharides

Wang

Zhu²,

Wang³

et al. 2023

Marine Drugs

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The enzymatic degradation of different chitin polymers into chitin oligosaccharides (COSs) is of great significance given their better solubility and various biological applications. Chitinase plays a pivotal role in the enzymatic preparation of COSs. Herein, a cold-adapted and efficient chitinase (ChiTg) from the marine Trichoderma gamsii R1 was purified and characterized. The optimal temperature of ChiTg was 40 °C, and the relative activity at 5 °C was above 40.1%. Meanwhile, ChiTg was active and stable from pH 4.0 to 7.0. As an endo-type chitinase, ChiTg exhibited the highest activity with colloidal chitin, then with ball-milled and powdery chitin. In addition, ChiTg showed high efficiency when hydrolyzing colloidal chitin at different temperatures, and the end products were mainly composed of COSs with one to three degrees of polymerization. Furthermore, the results of bioinformatics analysis revealed that ChiTg belongs to the GH18 family, and its acidic surface and the flexible structure of its catalytic site may contribute to its high activity in cold conditions. The results of this study provide a cold-active and efficient chitinase and ideas for its application regarding the preparation of COSs from colloidal chitin.

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“…Enzymatic hydrolysis can also be applied for cleaning of shrimp shells and bio-conversing them into valuable nitrogen-containing compounds, although intact shells are poorly hydrolyzed by chitinases unless pre-demineralization and deproteinization with chemicals or proteases is conducted [25]. To facilitate the hydrolysis of crayfish shell into oligosaccharides, ball milling and ultrasound-assisted hydrogen peroxide decolorization is applied for pretreating [26].…”

Section: Cleaning Shellsmentioning

confidence: 99%

Shell Waste Management and Utilization: Mitigating Organic Pollution and Enhancing Sustainability

Popović

Lorencin²,

Strunjak‐Perović

et al. 2023

Applied Sciences

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Every year, close to 8 million tons of waste crab, shrimp and lobster shells are produced globally, as well as 10 million tons of waste oyster, clam, scallop and mussel shells. The disposed shells are frequently dumped at sea or sent to landfill, where they modify soils, waters and marine ecosystems. Waste shells are a major by-product, which should become a new raw material to be used to the best of their potential. There are a number of applications for waste shells in many fields, such as agriculture, medicine, chemical production, construction, environmental protection, cosmetic industry, food and feed industry, and a plethora of other (often niche) applications, which are being developed by the day. This review provides a broad picture of crustacean and mollusc shell waste management and reutilization possibilities, reviewing well established, current, and potential strategies, particularly from the standpoint of sustainability challenges and energy demand.

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Characterization of chitinase from Exiguobacterium antarcticum and its bioconversion of crayfish shell into chitin oligosaccharides

Cited by 17 publications

References 55 publications

Chitinase-Assisted Bioconversion of Chitinous Waste for Development of Value-Added Chito-Oligosaccharides Products

Chitinase-Assisted Bioconversion of Chitinous Waste for Development of Value-Added Chito-Oligosaccharides Products

Biochemical Properties of a Cold-Active Chitinase from Marine Trichoderma gamsii R1 and Its Application to Preparation of Chitin Oligosaccharides

Shell Waste Management and Utilization: Mitigating Organic Pollution and Enhancing Sustainability

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