Characterization of chitin extracted from enzymatically deproteinized Acetes shell residue with varying degree of hydrolysis

Dhanabalan, Vignaesh; Xavier, K.A. Martin; Eppen, Shibu; Joy, Adharsh; Balange, Amjad Khansaheb; Asha, K.K.; Murthy, L.N.; Nayak, Binaya Bhusan

doi:10.1016/j.carbpol.2020.117203

Cited by 34 publications

(12 citation statements)

References 31 publications

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“…The values of residual minerals obtained in our scale-up studies are within the ash content range of different works employing the traditional solution-based process (0.4− 5%) but are still higher than the values of 0% obtained using the enzymatic approach. 71,[76][77][78][79][80]84 Further scale-up of the methodology proposed was performed using 200 g of GC shells and citric acid, in the blender. After 30 min of blending, deproteinization and demineralization of shells was achieved as seen by PXRD, TGA, and 13 C ssNMR analyses (Figures 4 and S14).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Extraction of Chitin from Green Crab Shells by Mechanochemistry and Aging

Hajiali

Vidal

Jin

et al. 2022

ACS Sustainable Chem. Eng.

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Crustacean shell waste is one of the most important chitin sources for commercial use due to its low price and high availability. The extraction of chitin from this residue relies on two removal steps: one for proteins and another one for minerals. Herein, we report a "solvent-free", one-pot process relying on mechanochemistry and aging to convert crustacean shells into chitin with high yields and low ash content. The extraction was performed on European Green Crabs, one of the world's most damaging invasive species, thus converting a serious environmental threat into a biomaterial opportunity. Successful chitin isolation of Green Crabs was achieved by milling of solid acids (i.e., citric, ascorbic, malic, succinic, and salicylic acid) for 10 to 30 min, while a combination of milling and aging was necessary for aqueous acids (i.e., hydrochloric and acetic acid). Milling, aging times, and shell to acid ratios were optimized, while the process could be scaled to 200 g of shell starting materials. This method required limited chemical and energy inputs, which were quantified by sustainability metrics. The process reported is a more sustainable approach for chitin production over the current industrial methods and has the potential to be extended to other chitin sources (e.g., lobster, shrimp, and insects).

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Section: ■ Results and Discussionmentioning

confidence: 99%

Extraction of Chitin from Green Crab Shells by Mechanochemistry and Aging

Hajiali

Vidal

Jin

et al. 2022

ACS Sustainable Chem. Eng.

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“…It is important to note here that refluxing is critical in the above steps in order to minimize the formation of corrosive effluents in the view of sustainable process. 43,47,48 Subsequently, the samples were treated to vacuum filtration, washed thoroughly with water, and dried in an oven for 10 h. The end product obtained at this stage was chitin powder. Finally, in the deacetylation step, the chitin powder was treated with 30% (w/w) aqueous NaOH, refluxing at 110 °C for 2 h with a chitin-to-solution ratio of 1:20 (w/v).…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

A Facile Method for Processing Durable and Sustainable Superhydrophobic Chitosan-Based Coatings Derived from Waste Crab Shell

Roy

Goh

Verma

et al. 2022

ACS Sustainable Chem. Eng.

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The substitution of synthetic raw materials with renewable feedstock is fueling a lot of interest to drive new scientific and technical advancements that underpin this strategy. In this context and looking at the broad prospect of chitosan, herein, we proposed a facile, economical, and novel strategy to process high-yield (44% ± 3) chitosan from waste crab shells and apply the processed chitosan to fabricate a durable, multifunctional, ecofriendly superhydrophobic coating. The coating was developed by binding the amines of chitosan and a long-chain polymer, octadecylamine using a cross-linking agent (glutaraldehyde). Applying the coating onto intrinsically hydrophilic polyester fabric and typical surgical-grade cotton yielded superhydrophobic materials with water contact angles of 158.6 and 161.4°(± 3), respectively. A preliminary study revealed that the coating could endure multiple cycles of laundry (60×), sandpaper abrasion (55×), and tape peel test (80×) with little diminution in the superhydrophobic property. Interestingly, any decrease in the superhydrophobicity could be restored after ironing the fabrics for 2 min, indicating that the effect was reversible. Furthermore, the coating could transform an ordinary hydrophilic polyurethane sponge into an excellent superhydrophobic− superoleophilic absorbent, which can rapidly absorb and separate a variety of organic solvents and oils from oil/water mixtures.

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“…29 However, it is well-acknowledged that chitin extracted using biological methods contains residual protein ranging from 3-10% while chemical extraction can lower the residual to <1%. 3,[53][54][55] Residual proteins are of concern, especially for biomedical and nanomaterial applications. 29 Jeuniaux et al proposed that residual protein in biologically extracted chitin is due to CBP which are covalently bound to chitin.…”

Section: Chitin Extractionmentioning

confidence: 99%

“…29 However, it is well-acknowledged that chitin extracted using biological methods contains residual protein ranging from 3–10% while chemical extraction can lower the residual to <1%. 3,53–55 Residual proteins are of concern, especially for biomedical and nanomaterial applications. 29…”

Section: Introductionmentioning

confidence: 99%