Characterization of chemoselective surface attachment of the cationic peptide melimine and its effects on antimicrobial activity

Chen, Renxun; Willcox, Mark; Cole, Nerida; Ho, Kaitlyn; Rasul, Riaz; Denman, John A.; Kumar, Naresh

doi:10.1016/j.actbio.2012.07.029

Cited by 53 publications

(51 citation statements)

References 39 publications

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“…However, this does not correlate with our results, nor with the aforementioned report by Chen et al, in Ref. [33]. Our results demonstrated that immobilized Dhvar5 lost part of their antimicrobial effect against S. aureus independently of their immobilization orientation.…”

Section: Discussioncontrasting

confidence: 57%

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Dhvar5 antimicrobial peptide (AMP) chemoselective covalent immobilization results on higher antiadherence effect than simple physical adsorption

et al. 2015

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a b s t r a c tBacterial colonization and subsequent biofilm formation is still one of the major problems associated with medical devices. Antimicrobial peptides (AMP) immobilization onto biomaterials surface is a promising strategy to avoid bacterial colonization. However, a correct peptide orientation and exposure from the surface is essential to maintain AMP antimicrobial activity.This work aims to evaluate the effect of the immobilization on antibacterial activity of Dhvar5 (LLLFLLKKRKKRKY), an AMP with a head-to-tail amphipathicity. Dhvar5 was linked to thin chitosan coatings in i) a controlled orientation and exposure, testing covalent immobilization of its N-or C-terminus and using spacers with different lengths and flexibilities or in ii) a random orientation by physical adsorption. Chitosan coating was chosen due to its antimicrobial properties and readiness to be functionalized.Surface characterization demonstrated the chemoselective immobilization of the peptide with different spacers in a similar concentration (~2 ng/cm 2 ).Efficacy assays demonstrated that covalent immobilization of Dhvar5 exposing its cationic end, improves the chitosan coating antimicrobial effect by decreasing Methicillin-resistant Staphylococcus aureus (MRSA) colonization. This effect was enhanced when longer spacers were used independently of their flexibility. In opposite, immobilized Dhvar5 exposing its hydrophobic end has no effect on bacterial adhesion to chitosan, and when adsorbed in a random orientation even induces bacterial adhesion to chitosan coating.

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Section: Discussioncontrasting

confidence: 57%

“…Recently, Chen et al [33], reported that melimine, a 28mer peptide with an N-terminus hydrophobic domain and cationic Cterminus, presented higher antimicrobial activity with a clear antiadherence effect when immobilized through its N-terminus, exposing the cationic domain away from the substrate. This comes in accordance with our results.…”

Section: Discussionmentioning

confidence: 99%

Dhvar5 antimicrobial peptide (AMP) chemoselective covalent immobilization results on higher antiadherence effect than simple physical adsorption

et al. 2015

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“…[322] The importance of ensuring the correct orientation of peptides has also been demonstrated in a study in which the antimicrobial activity of melamine, immobilized on maleimide-functionalized glass via an engineered cysteine, was found to vary greatly based on the position of the introduced cysteine. [323] Material surfaces onto which AMPs have been successfully tethered to date are highly diverse and include polymer brushes, [324] metals, [325] self-assembled monolayers [326] and contact lenses. [327] Many peptides, including AMPs, are synthesized directly onto the solid support using solid-phase peptide synthesis (SPPS) methods.…”

Section: Progress Reportmentioning

confidence: 99%

Adding Functions to Biomaterial Surfaces through Protein Incorporation

et al. 2016

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The concept of biomaterials has evolved from one of inert mechanical supports with a long-term, biologically inactive role in the body into complex matrices that exhibit selective cell binding, promote proliferation and matrix production, and may ultimately become replaced by newly generated tissues in vivo. Functionalization of material surfaces with biomolecules is critical to their ability to evade immunorecognition, interact productively with surrounding tissues and extracellular matrix, and avoid bacterial colonization. Antibody molecules and their derived fragments are commonly immobilized on materials to mediate coating with specific cell types in fields such as stent endothelialization and drug delivery. The incorporation of growth factors into biomaterials has found application in promoting and accelerating bone formation in osteogenerative and related applications. Peptides and extracellular matrix proteins can impart biomolecule- and cell-specificities to materials while antimicrobial peptides have found roles in preventing biofilm formation on devices and implants. In this progress report, we detail developments in the use of diverse proteins and peptides to modify the surfaces of hard biomaterials in vivo and in vitro. Chemical approaches to immobilizing active biomolecules are presented, as well as platform technologies for isolation or generation of natural or synthetic molecules suitable for biomaterial functionalization.

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“…The physicochemical characteristics of AMPs enable them to associate with negatively charged microbial membranes resulting in a lethal disruption in cell membrane [2]. In recent works, covalent immobilization of AMPs on different solid supports has been discussed for long-term stability and accessibility towards bacterial cells [3,[12][13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Antibacterial activity on electrospun poly(lactide-co-glycolide) based membranes via Magainin II grafting

Yüksel¹,

Karakeçili²

2014

Materials Science and Engineering: C

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Characterization of chemoselective surface attachment of the cationic peptide melimine and its effects on antimicrobial activity

Cited by 53 publications

References 39 publications

Dhvar5 antimicrobial peptide (AMP) chemoselective covalent immobilization results on higher antiadherence effect than simple physical adsorption

Dhvar5 antimicrobial peptide (AMP) chemoselective covalent immobilization results on higher antiadherence effect than simple physical adsorption

Adding Functions to Biomaterial Surfaces through Protein Incorporation

Antibacterial activity on electrospun poly(lactide-co-glycolide) based membranes via Magainin II grafting

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