Characterization of Cellulose synthase-like D (CSLD) family revealed the involvement of PtrCslD5 in root hair formation in Populus trichocarpa

Peng, Xiaopeng; Pang, Hongying; Abbas, Manzar; Yan, Xiaojing; Dai, Xinren; Li, Yun; Li, Quanzi

doi:10.1038/s41598-018-36529-3

Cited by 18 publications

(20 citation statements)

References 53 publications

(93 reference statements)

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“…The plant cell wall consists of polysaccharides (cellulose, hemicellulose and pectin), proteins and other compounds. It plays critical roles in the maintenance of cell integrity, and the regulation of many developmental processes in plants [ 1 , 2 , 3 , 4 , 5 , 6 ]. The cell wall represents not only a mechanical barrier, but also a signaling component during plant responses to various biotic [ 7 , 8 ] and abiotic stresses [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

“…Plant Csl genes play substantial roles in developmental processes, such as root hair formation [ 6 ], the control of organ size [ 4 ], tiller number [ 3 ] and the maintenance of adherent mucilage structure [ 1 , 2 , 5 ]. Csl genes were also reported to be involved in plant resistance/tolerance to biotic or abiotic stresses, such as salt [ 35 , 36 ], boron (B) [ 37 , 38 ] or heavy metal [ 36 , 39 ] stress, as well as pathogen infection [ 32 , 40 , 41 ].…”

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Identification of Banana Csl Gene Family and Their Different Responses to Low Temperature between Chilling-Sensitive and Tolerant Cultivars

Yuan

Liu

Takáč

et al. 2021

Plants

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The cell wall plays an important role in responses to various stresses. The cellulose synthase-like gene (Csl) family has been reported to be involved in the biosynthesis of the hemicellulose backbone. However, little information is available on their involvement in plant tolerance to low-temperature (LT) stress. In this study, a total of 42 Csls were identified in Musa acuminata and clustered into six subfamilies (CslA, CslC, CslD, CslE, CslG, and CslH) according to phylogenetic relationships. The genomic features of MaCsl genes were characterized to identify gene structures, conserved motifs and the distribution among chromosomes. A phylogenetic tree was constructed to show the diversity in these genes. Different changes in hemicellulose content between chilling-tolerant and chilling-sensitive banana cultivars under LT were observed, suggesting that certain types of hemicellulose are involved in LT stress tolerance in banana. Thus, the expression patterns of MaCsl genes in both cultivars after LT treatment were investigated by RNA sequencing (RNA-Seq) technique followed by quantitative real-time PCR (qPCR) validation. The results indicated that MaCslA4/12, MaCslD4 and MaCslE2 are promising candidates determining the chilling tolerance of banana. Our results provide the first genome-wide characterization of the MaCsls in banana, and open the door for further functional studies.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification of Banana Csl Gene Family and Their Different Responses to Low Temperature between Chilling-Sensitive and Tolerant Cultivars

Yuan

Liu

Takáč

et al. 2021

Plants

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“…To find out if our discoveries could be applied to other species, we performed a cross-species complementation experiment which showed that AtCSLD2 and AtCSLD3 (KOJAK), required for root hair development in Arabidopsis (Bernal et al, 2008), are functionally equivalent to LjCSLD1. In Populus trichocarpa PtrCslD5 is considered a functional orthologue of AtCLSD3 and along with the highly homologous PtrCslD6, it is also involved in root hair development (Peng et al, 2019). Similarly, rice OsCSLD1 is also required for root hair morphogenesis (Kim et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Intragenic Complementation ofCellulose Synthase-Like D1alleles in Root Hair Development

Karas

Ross

Novero

et al. 2020

Preprint

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Root hair cells form the primary interface of plants with the soil environment, playing key roles in nutrient uptake and plant defense. In addition, they are typically the first cells infected by nitrogen-fixing soil bacteria during the root nodule symbiosis. Here we report a role for the Cellulose Synthase-Like D1 (CSLD1) gene in root hair development in Lotus japonicus. CSLD1 belongs to the cellulose synthase protein family that includes cellulose synthases, and cellulose synthase-like proteins, the latter thought to be involved in the biosynthesis of hemicellulose. We describe 11 csld1 mutant alleles that have either short (Ljcsld1-1) or variable length roots hairs (Ljcsld1-2 to 11). Examination of Ljcsld1-1 and one variable-length root hair mutant, Ljcsld1-6 showed increased root hair cell wall thickness, which in Ljcsld1-1 was more pronounced, suggesting a possible link with the defect in root nodule symbiosis. In addition, Ljcsld1-1 heterozygotes had intermediate root hair lengths, between those of wild type and the homozygotes. Intragenic complementation was observed between alleles with mutations in the N-terminal domain and other alleles, suggesting modularity of CSLD1 function and that it may operate as a homodimer or multimer.One sentence summaryThis research describes novel gain- and loss-of-function mutations at the Lotus japonicus CELLULOSE SYNTHASE-LIKE D1 locus and analyzes their impact on root hair development.

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“…In addition, DcCslD5 was in clade III as well as functions of OsCslD4, PtrCslD2 and AtCslD5 were studied, which mutants displayed educe stem growth and synthesis of polysaccharides [49,50]. In clade IV, AtCslD6, PtrCslD3, PtrCslD4 and PAXXG254610 shared the same lineage; none of those were identified for their functions [33]. Based on the phylogenetic tree analysis, DcCslD2a, DcCslD2b, DcCslD3a and…”

Section: Phylogenetic Analysis and Classification Of Csld Proteins Inmentioning

confidence: 99%

“…It is conserved in all land plants, and among the ten Csl subfamilies, it shows the highest sequence similarity to the cellulose synthase genes, suggesting that it plays fundamental roles in plant development [25,26]. Many studies have described that CslDs are involved in various aspects of the plant life cycle, including synthesis of polysaccharides [27][28][29], environmental stimuli response [30], the expansion and division of cells [31][32][33], plant development [34][35][36][37] and so on. Over the past few years, the CslD family genes have been identified from the genome of various species, such as Arabidopsis thaliana [17,32], Populus trichocarpa [38], Zea mays L [31], Dendrobium catenatum [28,29],…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification of Cellulose-Like Synthase D Gene Family in Dendrobium Catenatum

Chen

et al. 2021

Preprint

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Background: Dendrobium catenatum, which grows on the semi humid rocks in the mountains, has been at the top of the "Nine Immortals of China" since ancient times. It is a kind of yin tonic medicine and its main active component is polysaccharide. Cellulose synthase-like D(CslD) genes were predicted to catalyse the biosynthesis of 1,4-β-d-glycan backbone of hemicelluloses, which plays fundamental roles in plant development. Results: To investigate the role of CslD in the development of Dendrobium catenatum, eight CslD genes (DcCslD1,2a,2b,3a,3b,4a,4b,5) were identified. The results of protein prediction and analysis showed that CslD2a/2b/4a/4b proteins were acidic proteins, the rest were basic proteins; Leu, Ser, Ala, Gly, Arg, Pro and Asp were the main amino acids. All the proteins had obvious hydrophobic or hydrophilic regions, and had transmembrane structure. The main elements of protein secondary structure were α-helix, random coil and extended chain. Phylogenetic analysis revealed that the DcCslD family could be divided into Ⅰ, Ⅱ, Ⅲ and Ⅳ groups. DcCslD proteins had typical Cellulose synthase domain and similar protein structures to the CslDs of other plants. Their promoter regions contain cis regulatory elements related to stress and hormone. The results of qRT-PCR showed that the identified DcCslDs were differentially expressed in roots, stems and leaves. Most of them hightly expressed in stems and leaves. What’s more, the environmental stress1es examination showed that the expressions of DcCslD5 were closely associated with drought-recovery treatment, the expression of DcCslD1, DcCslD2a, DcCslD2b, DcCslD3a, and DcCslD5 were significantly influenced by low temperature. Conclusions: This study systematically analyzed the sequence characteristics of CslD protein of D. catenatum, which can provide reference for further study on the function of CslD protein in polysaccharide metabolism of D. catenatum.

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Characterization of Cellulose synthase-like D (CSLD) family revealed the involvement of PtrCslD5 in root hair formation in Populus trichocarpa

Cited by 18 publications

References 53 publications

Genome-Wide Identification of Banana Csl Gene Family and Their Different Responses to Low Temperature between Chilling-Sensitive and Tolerant Cultivars

Genome-Wide Identification of Banana Csl Gene Family and Their Different Responses to Low Temperature between Chilling-Sensitive and Tolerant Cultivars

Intragenic Complementation ofCellulose Synthase-Like D1alleles in Root Hair Development

Genome-Wide Identification of Cellulose-Like Synthase D Gene Family in Dendrobium Catenatum

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