Characterization of Cellulose-Degrading Bacteria Isolated from Soil and the Optimization of Their Culture Conditions for Cellulase Production

Kognou, Aristide Laurel Mokale; Chio, Chonlong; Khatiwada, Janak Raj; Shrestha, Sarita; Chen, Xuantong; Han, Song; Li, Hongwei; Jiang, Zi‐Hua; Xu, Chunbao; Qin, Wensheng

doi:10.1007/s12010-022-04002-7

Cited by 18 publications

(7 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MKAL5, and Bacillus sp. MKAL6 with the NCBI accession numbers ON442553, ON442554, ON442555, ON442556, ON442557, and ON442558, respectively [Mokale et al, 2022a[Mokale et al, , 2022b. Bacteria were maintained in Tryptic soy broth (TSB) at 30°C for subsequent tests.…”

Section: Microorganismsmentioning

confidence: 99%

“…Six cellulolytic bacteria, Paenarthrobacter sp., Hymenobacter sp., Mycobacterium sp., Stenotrophomonas sp., Chryseobacterium sp., and Bacillus sp., isolated from isolated soil samples collected in Kingfisher Lake and the University of Manitoba campus showed promising activities in the fermentation process [Mokale et al, 2022a[Mokale et al, , 2022b. However, their innocuity has not been investigated yet, a primary characteristic in lignocellulosic biomass bioprocess industries.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of Potential Virulence, Resistance to Antibiotics and Heavy Metals, and Biofilm-Forming Capabilities of Soil Lignocellulolytic Bacteria

Kognou¹,

Chio²,

Khatiwada³

et al. 2023

Microb Physiol

Self Cite

View full text Add to dashboard Cite

Soil bacteria participate in self-immobilization processes for survival, persistence and producing virulence factors in some niches or hosts through their capacities of autoaggregation, cell surface hydrophobicity, biofilm formation, and antibiotic and heavy metal resistance. This study investigated potential virulence, antibiotics and heavy metals resistance, solvent adhesion, and biofilm-forming capabilities of six cellulolytic bacteria isolated from soil samples: Paenarthrobacter sp. MKAL1, Hymenobacter sp. MKAL2, Mycobacterium sp. MKAL3, Stenotrophomonas sp. MKAL4, Chryseobacterium sp. MKAL5 and Bacillus sp. MKAL6. Strains were subjected to phenotypic methods, including heavy metal and antibiotic susceptibility and virulence factors (protease, lipase, capsule production, autoaggregation, hydrophobicity and biofilm formation). The effect of ciprofloxacin was also investigated on bacterial susceptibility over time, cell membrane and biofilm formation. Strains MKAL2, MKAL5 and MKAL6 exhibited protease and lipase activities, while only MKAL6 produced capsules. All strains were capable of aggregating, forming biofilm and adhering to solvents. Strains tolerated high amounts of chromium, lead, zinc, nickel and manganese and were resistant to lincomycin. Ciprofloxacin exhibited bactericidal activity against these strains. Although the phenotypic evaluation of virulence factors of bacteria can indicate their pathogenic nature, an in-depth genetic study of virulence, antibiotic and heavy metal resistance genes is required.

show abstract

Section: Microorganismsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of Potential Virulence, Resistance to Antibiotics and Heavy Metals, and Biofilm-Forming Capabilities of Soil Lignocellulolytic Bacteria

Kognou¹,

Chio²,

Khatiwada³

et al. 2023

Microb Physiol

Self Cite

View full text Add to dashboard Cite

show abstract

“…According to the relevant literatures [16,17], we designed the isolation, screening, and optimization process (Figure 1). Soil samples (5 g) were diluted with 100 mL of sterile water and then transferred into a shaking incubator for 60 min at 30 • C; After incubation, microbial suspension (50 µL) was plated onto R2A agar.…”

Section: Screening and Isolationmentioning

confidence: 99%

“…The effect of different pH on the cellulase production of strain B13-2 is shown in Figure 7. The control of pH during fermentation is necessary to improve the productivity of cellulase [17]. When the pH was 4.0, the strain B13-2 produced the highest cellulase production (cellulase activity = 11.36 ± 0.15 U/mL) after 3 days of incubation.…”

Section: Effect Of Ph On Cellulase Productionmentioning

confidence: 99%

Isolation of an Acidophilic Cellulolytic Bacterial Strain and Its Cellulase Production Characteristics

et al. 2023

Self Cite

View full text Add to dashboard Cite

The aim of the study was to isolate and identify a highly efficient cellulolytic bacteria strain that can be used in acidic environments, and then investigate its cellulase production characteristics for the effective utilization of agricultural waste. For this purpose, we set a series of isolation and screening steps, 21 strains were isolated from soil, and an acidophilic strain labeled as B13-2 with high cellulase production was screened using the Gram’ iodine method and cellulase activity assay; it was identified as Raoultella terrigena. Lastly, the culture conditions such as incubation time, incubation temperature, pH, carbon sources, nitrogen sources, and inoculum size were optimized via single-factor experiments, and on this basis, the cellulase production of strain B13-2 was optimized using response surface methodology with cellulase activity as the optimization goal. The results of the response surface optimization showed that the optimum incubation time is 3.1 days, the optimum temperature is 29.9 °C, the optimum pH is 4.1, and the optimum inoculum size is 1.50%, the cellulase activity reached a maximum of 13.503 U/mL, which was about 140% higher than that before optimization. In particular, strain B13-2 had higher cellulase production when rice straws were used as the natural carbon source. Meanwhile, the SEM pictures demonstrated that the surface of the substrate rice straws in an acidic buffer with strain B13-2 was uneven, with larger holes than in the neutral buffer after incubation. It further proved that this strain has a stronger ability to degrade cellulose under acidic conditions. The B13-2 is a kind of acidophilic cellulolytic bacteria. Therefore, it has the potential to be developed into a silage additive agent and provides a high-quality strain resource for the high-value biotransformation of agricultural waste and lays a certain foundation for the sustainable development of agricultural cultivation.

show abstract

“…Bacillus sp. has been reported to be effective at degrading cellulose [ 1 , 2 , 3 ]. However, although selenium can significantly improve the nutritional quality of feed, there are few reports on its decomposition of carboxymethyl cellulose (CMC) [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Enhancing the Activity of Carboxymethyl Cellulase Enzyme Using Highly Stable Selenium Nanoparticles Biosynthesized by Bacillus paralicheniformis Y4

Wang

Duan

et al. 2022

Molecules

View full text Add to dashboard Cite

The inorganic selenium is absorbed and utilized inefficiently, and the range between toxicity and demand is narrow, so the application is strictly limited. Selenium nanoparticles have higher bioactivity and biosafety properties, including increased antioxidant and anticancer properties. Thus, producing and applying eco-friendly, non-toxic selenium nanoparticles in feed additives is crucial. Bacillus paralicheniformis Y4 was investigated for its potential ability to produce selenium nanoparticles and the activity of carboxymethyl cellulases. The selenium nanoparticles were characterized using zeta potential analyses, Fourier transform infrared (FTIR) spectroscopy, and scanning electron microscopy (SEM). Additionally, evaluations of the anti-α-glucosidase activity and the antioxidant activity of the selenium nanoparticles and the ethyl acetate extracts of Y4 were conducted. B. paralicheniformis Y4 exhibited high selenite tolerance of 400 mM and the selenium nanoparticles had an average particle size of 80 nm with a zeta potential value of −35.8 mV at a pH of 7.0, suggesting that the particles are relatively stable against aggregation. After 72 h of incubation with 5 mM selenite, B. paralicheniformis Y4 was able to reduce it by 76.4%, yielding red spherical bio-derived selenium nanoparticles and increasing the carboxymethyl cellulase activity by 1.49 times to 8.96 U/mL. For the first time, this study reports that the carboxymethyl cellulase activity of Bacillus paralicheniforis was greatly enhanced by selenite. The results also indicated that B. paralicheniformis Y4 could be capable of ecologically removing selenite from contaminated sites and has great potential for producing selenium nanoparticles as feed additives to enhance the added value of agricultural products.

show abstract

Characterization of Cellulose-Degrading Bacteria Isolated from Soil and the Optimization of Their Culture Conditions for Cellulase Production

Cited by 18 publications

References 63 publications

Characterization of Potential Virulence, Resistance to Antibiotics and Heavy Metals, and Biofilm-Forming Capabilities of Soil Lignocellulolytic Bacteria

Characterization of Potential Virulence, Resistance to Antibiotics and Heavy Metals, and Biofilm-Forming Capabilities of Soil Lignocellulolytic Bacteria

Isolation of an Acidophilic Cellulolytic Bacterial Strain and Its Cellulase Production Characteristics

Enhancing the Activity of Carboxymethyl Cellulase Enzyme Using Highly Stable Selenium Nanoparticles Biosynthesized by Bacillus paralicheniformis Y4

Contact Info

Product

Resources

About