Characterization of cellulases and hemicellulases produced by Trichoderma reesei on various carbon sources

Juhász, Tamás; Szengyel, Zsolt; Réczey, Kati; Siika‐aho, Matti; Viikari, Liisa

doi:10.1016/j.procbio.2005.03.057

Cited by 234 publications

(147 citation statements)

References 22 publications

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“…A submerged fungal culture is recognized as a complex multiphase, multicomponent process where cell growth and product formation are influenced by a large number of operating parameters, such as culture broth composition, temperature, pH, shear stress, initial inoculum, dissolved oxygen, rheology and fungal morphology (Patel et al, 2009). It is a well-established fact that optimization of culture medium and culture conditions influence enzyme production (Juhász et al, 2005;Shanmugam et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

STATISTICAL OPTIMIZATION OF MINERAL SALT AND UREA CONCENTRATION FOR CELLULASE AND XYLANASE PRODUCTION BY Penicillium echinulatum IN SUBMERGED FERMENTATION

Reis

Ritter

Fontana

et al. 2015

Braz. J. Chem. Eng.

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-Penicillium echinulatum S1M29 is a mutant with cellulase and xylanase production comparable to the most studied microorganisms in the literature. However, its potential to produce these enzymes has not been fully investigated. This study aimed at optimizing salt and urea concentrations in the mineral solution, employing the response surface methodology. A 2 5-1 Fractional Factorial Design and a 2 3 Central Composite Design were applied to elucidate the effect of salts and urea in enzyme production. Lower concentrations of KH 2 PO 4 (2.0 g.L -1 ), (NH 4 ) 2 SO 4 (1.4 g.L -1 ), MgSO 4 .7H 2 O (0.375 g.L -1 ) and CaCl 2 (0.375 g.L -1 ) were most suitable for the production of all enzymes evaluated. Nevertheless, higher concentrations of urea (0.525 g.L -1 ) gave the best results for cellulase and xylanase production. The maximum FPase (1,5 U.m.L -1 ), endoglucanase (7,2 U.m.L -1 ), xylanase (30,5 U.m.L -1 ) and β-glucosidase (4,0 U.m.L -1 ) activities obtained with the planned medium were, respectively, 87, 16, 17 and 21% higher when compared to standard medium. The experimental design contributed to adjust the concentrations of minerals and urea of the culture media for cellulase and xylanase production by P. echinulatum, avoiding waste of components in the medium.

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Section: Introductionmentioning

confidence: 99%

STATISTICAL OPTIMIZATION OF MINERAL SALT AND UREA CONCENTRATION FOR CELLULASE AND XYLANASE PRODUCTION BY Penicillium echinulatum IN SUBMERGED FERMENTATION

Reis

Ritter

Fontana

et al. 2015

Braz. J. Chem. Eng.

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“…The structure of lignocellulose is comprised of tightly associated cellulose, hemicellulose and lignin polymers [2]. Generally, such biomass is composed of approximately 40% cellulose, 20-30% lignin and 20-30% hemicellulose, the most abundant of which is xylan [3].…”

Section: Introductionmentioning

confidence: 99%

Purification and characterisation of a xylanase from Thermomyces lanuginosus and its functional expression by Pichia pastoris

Gaffney

Carberry

Doyle

et al. 2009

Enzyme and Microbial Technology

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a b s t r a c tA xylanase produced by Thermomyces lanuginosus 195 by solid state fermentation (SSF) was purified 9.3-fold from a crude koji extract, with a 7.6% final yield. The purified xylanase (with an estimated mass of 22 kDa by SDS-PAGE) retained 18% relative activity when treated for 10 min at 100• C and approximately 90% relative activity when incubated at pH values ranging from 6 to 10. Xylanase activity in the purified preparation was significantly enhanced following treatment with manganese and potassium chlorides (p < 0.05) but significantly reduced by calcium, cobalt and iron (p < 0.05). The purified enzyme was also shown to be exclusively xylanolytic. The gene encoding xylanase activity from T. lanuginosus 195 was functionally expressed by Pichia pastoris. MALDI-ToF mass spectrometry and zymography were employed to confirm functional recombinant expression. Maximum xylanase titres were achieved following 120 h induction of the recombinant culture, yielding 26.8 U/mL. Achieving functional protein expression facilitates future efforts to optimise the cultivation conditions for heterologous xylanase production.

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“…Different T. aurantiacus strains show endoglucanases with acid pI (around 3.5-3.7) (Hong et al, 2007;Parry et al, 2001) that display high stability (at 70°C half live of 98 h) (Gomes et al, 2000), optimum temperature and pH between 65 and 80°C and 4.0 and 5.5, respectively (Parry et al, 2001;Kalogeris et al, 2003). Besides producing cellulases, T. aurantiacus can secret most of the hemicellulolytic enzymes, endo-xylanase being the main enzyme detected in its culture, similar to several other well known hemicellulase-producing microorganisms such as Aspergillus niger (Bailey and Poutanen, 1989;Kang et al, 2004;Coral et al, 2002) and Trichoderma reesei (Juhasz et al, 2005).…”

Section: Introductionmentioning

confidence: 94%

Use of a Mixture of Thermophilic Enzymes Produced by the Fungus <i>Thermoascus aurantiacus</i> to Enhance the Enzymatic Hydrolysis of the Sugarcane Bagasse Cellulose

Monte¹

2010

American Journal of Agricultural and Biological Sciences

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Problem statement:The production of hydrolytic enzymes by T. aurantiacus has been performed under solid-state fermentations using lignocellulosic materials. The influences of the inoculum size and of the fermentation medium on the production of hemicellulases and cellulases were studied. Filtrates from the cultures were used to hydrolyze a pulp of sugarcane bagasse and the produced enzymes were shown to be candidates for use as co-adjuvants in plant saccharification. Aproach: The present study focuses on the effect of different culture conditions on production of cellulases and hemicellulases by T. aurantiacus. It is also provides a possible application of T. aurantiacus enzymes in the degradation of sugarcane bagasse pulp, considering that this thermophilic fungus is a potential source of thermostable enzymes. Results: T. aurantiacus was cultivated on four different agricultural residues: sugarcane bagasse, sugarcane straw, wheat straw and corn cob. Xylanase was produced with much more expressive activity than cellulases. The highest titre of xylanase was obtained on sugarcane straw at 9 days (1679.8 IU g , the amount of exoglucanase produced by the fungus considerably exceeds that produced with 10 4 spores g −1 . Xylanases and cellulases purified from filtrates of the cultures were investigated to hydrolyze a bagasse pulp prepared with alkaline peroxide. Xylanase or sulphuric acid were used as pretreatments for xylan removal, increasing the cellulase performance on pulp bagasse. However, results revealed that the removal of hemicellulose is not the only main factor limiting the cellulose hydrolysis. Conclusion: Results indicate that the xylanase action on alkaline-pretreated sugar cane bagasse enhances the cellulolytic effect promoted by a commercial cellulase. This study thus presents an evaluation of the applicability of enzymes from Thermoascus aurantiacus to potentially improve the enzymatic cellulose hydrolysis.

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Characterization of cellulases and hemicellulases produced by Trichoderma reesei on various carbon sources

Cited by 234 publications

References 22 publications

STATISTICAL OPTIMIZATION OF MINERAL SALT AND UREA CONCENTRATION FOR CELLULASE AND XYLANASE PRODUCTION BY Penicillium echinulatum IN SUBMERGED FERMENTATION

STATISTICAL OPTIMIZATION OF MINERAL SALT AND UREA CONCENTRATION FOR CELLULASE AND XYLANASE PRODUCTION BY Penicillium echinulatum IN SUBMERGED FERMENTATION

Purification and characterisation of a xylanase from Thermomyces lanuginosus and its functional expression by Pichia pastoris

Use of a Mixture of Thermophilic Enzymes Produced by the Fungus <i>Thermoascus aurantiacus</i> to Enhance the Enzymatic Hydrolysis of the Sugarcane Bagasse Cellulose

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