Characterization of cellular pathways involved in glioblastoma response to the chemotherapeutic agent 1, 3-bis(2-chloroethyl)-1-nitrosourea (BCNU) by gene expression profiling.

Rhee, Chang Hun; Ruan, Sanbao; Chen, S.; Chenchik, Alex; Levin, Victor A.; Yung, Alfred; Fuller, Gregory N.; Zhang, Wei

doi:10.3892/or.6.2.393

Cited by 5 publications

(1 citation statement)

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“…-El efecto citostático del BCNU puede estar en relación con el factor de necrosis tumoral. Este hecho se ha observado en líneas celulares de glioblastoma en el que las líneas respondedoras mostraban una marcada inducción de dicho factor, a diferencia de las que eran refractarias al tratamiento [47].…”

Section: Aspectos Moleculares De Interés En Los Ependimomas Los Aspecunclassified

Actualización sobre la biología molecular de los gliomas: hacia una clasificación patomolecular de los gliomas

Idoate

Echeveste²

2007

RevNeurol

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Section: Aspectos Moleculares De Interés En Los Ependimomas Los Aspecunclassified

Actualización sobre la biología molecular de los gliomas: hacia una clasificación patomolecular de los gliomas

Idoate

Echeveste²

2007

RevNeurol

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Genomics-based hypothesis generation: a novel approach to unravelling drug resistance in brain tumours?

Bredel

Šikić

2004

The Lancet Oncology

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BCNU Down-Regulates Anti-Apoptotic Proteins Bcl-xL and Bcl-2 in Association with Cell Death in Oligodendroglioma-Derived Cells

et al. 2004

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Oligodendroglial differentiation in gliomas is associated with enhanced sensitivity to chemotherapy. Antiapoptotic proteins, Bcl-xL and Bcl-2, are over-expressed in early passage cell lines derived from glioblastomas. Down-regulation of Bcl-xL and Bcl-2 with DNA antisense oligonucleotides promotes cell death in glioblastoma cells. Changes of expression of Bcl-xL and Bcl-2 after chemotherapy treatment have not been studied in glioma subtypes. The current experiments correlate decreased expression of both Bcl-xL and Bcl-2 after BCNU chemotherapy and cell death in two oligodendroglioma-derived cell lines. Expression of Bcl-2 family member proteins Bcl-xL, Bcl-2, and Bax were assessed in glioma cells both before and after chemotherapy treatment. Cell survival was assessed with a crystal violet bioassay. Levels of expression of Bcl-2 and Bcl-xL were elevated in two early passage oligodendroglioma-derived cell lines compared with a non-neoplastic glial cell line. BCNU chemotherapy markedly down-regulated expression of Bcl-xL and Bcl-2 proteins in both oligodendroglioma-derived cell lines. Changes in expression of Bcl-xL and Bcl-2 were associated with the increased sensitivity to chemotherapy. There were no changes noted in expression of Bax after BCNU treatment. Modulation of expression of the anti-apoptotic proteins, Bcl-xL and Bcl-2, in the two oligodendroglioma-derived cell lines was associated with increased sensitivity to BCNU chemotherapy. Down-regulation of Bcl-xL and Bcl-2 resulted in reversal of the ratio of Bax/Bcl-xL and Bax/Bcl-2 and enhanced cell death after treatment with BCNU. Mechanisms that control expression of the anti-apoptotic proteins Bcl-xL and/or Bcl-2 may be effective targets in treatment strategies in patients with malignant gliomas.

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Characterization of cellular pathways involved in glioblastoma response to the chemotherapeutic agent 1, 3-bis(2-chloroethyl)-1-nitrosourea (BCNU) by gene expression profiling.

Cited by 5 publications

References 0 publications

Actualización sobre la biología molecular de los gliomas: hacia una clasificación patomolecular de los gliomas

Actualización sobre la biología molecular de los gliomas: hacia una clasificación patomolecular de los gliomas

Genomics-based hypothesis generation: a novel approach to unravelling drug resistance in brain tumours?

BCNU Down-Regulates Anti-Apoptotic Proteins Bcl-xL and Bcl-2 in Association with Cell Death in Oligodendroglioma-Derived Cells

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